Lentivirus vectors

GFP gene under control of CMV to retinal cells by injection into the subretinal space of eyes in rats the GFP gene was efficiently expressed in both photoreceptor cells and retinal pigment epithelium predominant expression in photoreceptor cells was achieved using the rhodopsin promoter. The transduction efficiency was high and photoreceptor cells in >80% of the area of whole retina were expressing GFP (76). Intron-containing constructs have been successfully introduced into recent versions of lentivirus vectors (77). 

Recently, a series of lentivirus vectors were developed for transduction of hepa-tocytes in vivo (78). Various promoters, such as the human CMV, the human phos-phoglycerate kinase (PGK) and the mouse albumin promoter, were introduced into the FlIV-1-based vector. These vectors showed enhanced nuclear translocation in hepatocytes and improved transgene expression. Interestingly, targeted expression to the liver could be accomplished by the use of the albumin promoter. Therapeutic levels of human factor IX were achieved after a single injection. 

Kafri, T. (2001). Lentivirus vectors difficulties and hopes before clinical trials. Cum Opin. Mol. Then, 3, 316-326. 

Sinn, P. L., Hickey, M. A., Staber, P. D., Dylla, D. E., Jeffers, S. A., Davidson, B. L., Sanders, D. A. and McCray, P. B., Jr. (2003). Lentivirus vectors pseudotyped with filoviral envelope glycoproteins transduce airway epithe-lia from the apical surface independently of folate receptor alpha. J. Virol. 77, 5902-5910. 

Bjorklund, A. et al. (2000). Towards a neuroprotective gene therapy for Parkinson s disease Use of adenovirus, AAV and lentivirus vectors for gene transfer of GDNF to the nigrostriatal system in the rat Parkinson model. Brain Res. 886(1-2), 82-98. 

Evans JT, Gai cia JV (2000) Lentivirus vector mobilization and spread by human immunodeficiency virus. Hum Gene Ther 11 2331-2339. 

Lentivirus vectors have also been used in the development of potential AD therapeutics. Dodart and colleagues recently delivered varying alleles of the apolipoprotein E (apoE) gene,... 

Desmaris N, Bosch A, Salaun C, Petit C, Prevost MC, Tordo N, Perrin P, Schwartz O, de Rocquigny H, Heard JM (2001) Production and neurotropism of lentivirus vectors pseudotyped with lys-savirus envelope glycoproteins. Mol Ther 4 149-156. 

Zufferey R, Dull T, Mandel RJ, Bukovsky A, Quiroz D, et al. 1998. Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J. Virol. 72 9873-80... 

Miyoshi H, Blomer U, Takahashi M, Gage FH, Verma IM. 1998. Development of a self-inactivating lentivirus vector. J. Virol. 72 8150-57... 

Dull T, ZufTerey R, Kelly M, Mandel RJ, Nguyen M, et al. 1998. A third-generation lentivirus vector with a conditional packaging system. J. Virol. 72 8463-71... 

Kafri T, van Praag H, Ouy ang L, Gage FH, Verma IM. 1999. A packaging cell line for lentivirus vectors. J. Virol. 73 576-84... 

Blomer U, Naldini L, Kafri T, Trono D, Verma IM, Gage FH. 1997. Highly efficient and sustained gene transfer in adult neurons with a lentivirus vector. J. Virol. 71 6641 19... 

HIV-2) encapsidation determinant and transduction of nondividing human cells by FHV-2-based lentivirus vectors. J. Virol. 72 6527-36... 

Schnell T, Foley P, Wirth M, Munch J, Liberia K. 2000. Development of a self-inactivating, minimal lentivirus vector based on simian immunodeficiency virus. Hum. Gene Ther. 11 439 17... 

Browning MT, Schmidt RD, Lew KA, Rizvi TA. 2001. Primate and feline lentivirus vector RNA packaging and propagation by heterologous lentivirus virions. J. Virol. 75 5129 10... 

White, S.M., et al. 1999. Lentivirus vectors using human and simian immunodeficiency virus elements. Virol 73, 2832-2840. 

Lee M T, et al. (2003). Inhibition of hnman immnnodeficiency virns type 1 replication in primary macrophages by using Tat- or CCR5-specific small interfering RNAs expressed from a lentivirus vector. J. Virol. 77(22) 11964-11972. 

Gene delivery vectors could be tailored to different clinical needs. Adenoviral vectors, for example, achieve the peak expression earlier than other vectors albeit in a transient manner. This would be ideal for the temporary pacing needs to treat infected pacemaker devices [10, 35, 38], On the other hand, the high immunogenicity of these vectors could be circumvented by using helper-dependent adenovirus [51]. Accordingly, adeno-associated virus or lentivirus vectors would be more suitable for long-term applications [52]. 

N. Castillon, A. Avril-Delplanque, C. Coraux, C. Delenda, B. Peault, O. Danos, E. Puchelle, Regeneration of a well-differentiated human airway surface epithelium hy spheroid and lentivirus vector-transduced airway cells, J. Gene Med. 6 (8) (August 2004) 846-856. 

Barry SC, Harder B, Brzezinski M, Flint LY, Seppen J, Osborne WR. Lentivirus vectors encoding both central polypurine tract and posttranscriptional regulatory element provide enhanced transduction and transgene expression. Hum Gen Ther 2001 12 1103-1108. 

Iyer M, Salazar FB, Lewis X, Zhang L, Carey M, Wu L, Gambhir SS. Noninvasive imaging of enhanced prostate-specific gene expression using a two-step transcriptional amplification-based lentivirus vector. Mol Ther 2004 10 545-552. 

Kafri, T., Gene delivery by lentivirus vectors an overview, Meth. Mol Biol, 246,367,2004. 

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