Efficiency transduction

Although this study provided insight into the clonal composition of human HSC hierarchy, it was unknown if the ex vivo manipulations required for retroviral marking had quantitative or qualitative effects on SRC fates. The developments in lentivector transduction protocols enabled efficient transduction of SRC in the absence of cytokine and serum stimulation . Using minimal ex vivo manipulation to mark hematopoietic cells with lentiviral integration, the fates of SRC were evaluated . As reported previously using retroviral-marking, lentivector transduced short-term SRC contributed rapidly... 

The comparison of the 7max values 24 and 13.1 pA/cm2 for HRP and GOX respectively seems to indicate a more efficient transduction using HRP. However, it should be noted that the immobilized amount of GOX-B is three times lower than that of HRP. Indeed, assuming that at... 

A. L. Peel, S. Zolotukhin, G. W. Schrimsher, N. Muzyczka, andP. J. Reier, Efficient transduction of green fluorescent protein in spinal cord neurons using adeno-associated virus vectors containing cell type-specific promoters, Gene Ther. 4 16... 

Ferrari, F. K., Samulski, T., Shenk, T. and Samulski, R. J. (1996). Second-strand synthesis is a rate-limiting step for efficient transduction by recombinant adeno-associated virus vectors. J. Virol. 70, 3227-3234. 

Halbert, C. L., Allen, J. M. and Miller, A. D. (2001). Adeno-associated virus type 6 (AAV6) vectors mediate efficient transduction of airway epithelial cells in mouse lungs compared to that of AAV2 vectors. J. Virol. 75, 6615-6624. 

Kaludov, N., Brown, K. E., Walters, R. W., Zabner, J. and Chiorini, J. A. (2001). Adeno-associated virus serotype 4 (AAV4) and AAV5 both require sialic acid binding for hemagglutination and efficient transduction but differ in sialic acid linkage specificity. J. Virol. 75, 6884-6893. 

McCarty, D. M., Monahan, P. E. and Samulski, R. J. (2001). Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis. Gene Ther. 8, 1248-1254. 

Su L, Lee R, Bonyhadi M, etal. Hematopoietic stem cell-based gene therapy for acquired immunodeficiency syndrome efficient transduction and expression of RevM 10 in myeloid cells in vivo and in vitro. Blood 1997 89(7) 2283-2290. 

FV, however, is unable to replicate in nondividing cells despite efficient nuclear localization of the preintegration complex due to a NLS in gag (79,80). Nevertheless, efficient transduction of postmitotic cells has been demonstrated using FV vectors in which transgene expression is driven by an internal CMV promotor (81), indicating that vims replication in nondividing cells is probably blocked at the transcriptional level. 

Poeschla EM, Wong-Staal F, Looney DJ. 1998. Efficient transduction of nondividing human cells by feline immunodeficiency virus lentiviral vectors. Nat. Med. 4 354-57... 

Davidoff AM, Gray JT, Ng CY, Zhang Y, Zhou J, Spence Y, Bakar Y, Nathwani AC. Comparison of the ability of adeno-associated viral vectors pseudotyped with serotype 2, 5, and 8 capsid proteins to mediate efficient transduction of the liver in murine and nonhuman primate models. Mol Ther 2005 11 875-888. 

While siRNA duplexes are transfected into cells and allow downregulation of a specific PTP for a short period of time (days), shRNA are introduced into cells through expression vectors, allowing long-term and regulated delivery in single cells and whole organisms [32]. However, efficient transduction of specific shRNA... 

This approach has received more attention because the protected cells have a selective advantage over the non-transduced ones. Therefore, the requirement for a very high efficient transduction rate is less critical. Protected T cells should be used to repopulate the immune system with virus-resistant cells, and gene transfer to hematopoietic stem cells (HSC) is a promising approach to achieve this goal [34] (see also Part II, Chapter 8). However, it is still unclear whether the repopulated immune system would be able to generate a diversity of anti-... 

Efficient transduction of a large variety of quiescent and actively dividing cells... 

As a comparison to the responses recorded in B. latro, we performed exactly the same type of EAG recordings in the fruitfly, Drosophila melanogaster. Here, we could observe identical kinetics in the two distant species, indicating the presence of an at least as efficient transduction mechanism in the crab as in the fly. Closer examinations of the EAG response dynamics also hint at a fast and efficient transduction pathway. 

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