Gene transfer

1990) have noted sensitization of transduced cells that is not easily reconciled with a scavenger role for metallothionein. It appears the drug-resistant phenotype depends on the cellular context in which metallothionein is expressed. Thus, the mechanism by which metallothionein modifies cellular sensitivity to anticancer drugs remains obscure. 

Human Tumor Expression of Metallothionein and Drug Sensitivity 

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Wolff JA, Malone RW, Williams P et al (1990). Direct gene transfer into mouse muscle in vivo. Science 247 1465-1468... 

Viral vectors Gene transfer Gene therapy... 

A major drawback of vectors derived from prototypic retroviruses is that they can only transduce dividing cells. Therefore, these vectors cannot be used for gene transfer in many nondividing cells (e.g., muscle and brain cells). 

Levine BL, Humeau LM, Boyer J et al (2006) Gene transfer in humans using a conditionally replicating lentiviral vector. Proc Natl Acad Sci USA 103(46) 17372-17377... 

Gene Expression Analysis Gene Gun Gene Products Gene Promoter Gene Therapy Gene-therapy Vectors Gene Transfer... 

Dickson, G., Dunckley, M. (1993). Human dystrophin gene transfer Genetic correction of dystrophin deficiency. In Molecular and Cell Biology of Muscular Dystrophy (Partridge, T., ed.), pp. 283-302, Chapman Hall, London. 

Clinical Trials Involving T Cell Gene Transfer of Antiviral Genes.279... 

Clinical Trials Involving Stem Cell Gene Transfer... 

HBV-based vectors efficiently target quiescent hepatocytes and HBV-specific promoter and enhancer elements allow hepatocyte specific gene expression (Protzer et al. 1999). In addition, a very favorable ratio of infectious to defective particles renders HBV-based vectors good candidates for liver-directed gene transfer. Improved HBV vectors, in which HBV gene expression was abolished (Untergasser and Protzer 2004), were used in chimpanzees to treat chronic HCV infection and did not show any toxicity (Shin et al. 2005). 

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