Human osteosarcoma

Cellular uptake of MTX in an MTX-LDH hybrid was quantified by flow cytometry with FITC-conjugated MTX (MTX-FTIC). Human osteosarcoma (HOS) cells (5 x 105/1 ml) were treated with 50 pg/mL of MTX-FITC and 112 pg/mL of MTX-FITC-LDH, where the concentration of MTX-FITC in MTX-FITC-LDH was adjusted to maintain an equivalent amount of free MTX-FITC treated. After 1, 2, and 5h, cellular uptake was about 55-fold higher for MTX-FITC-LDH than for free MTX-FITC, as determined by the remarkably high fluorescence intensity of MTX-FITC-LDH compared to free MTX-FITC (Figure 13.6). This is evidence that MTX delivery in cells is greatly enhanced by using the MTX-LDH hybrid. 

Miller AC, Kariko K, Myers CE, et al. Increased radioresistance of EJras-transformed human osteosarcoma cells and its modulation by lovastatin, an inhibitor of p21ras isoprenylation. Int J Cancer 1993 53 302-307. 

Bavelloni, A. Santi, S. Sirri, A. Riccio, M. Faenza, L Zini, N. Cecchi, S. Ferri, A. Auron, P. Maraldi, N.M. Marmiroli, S. Phosphatidylinositol 3-kinase translocation to the nucleus is induced by interleukin 1 and prevented by mutation of interleukin 1 receptor in human osteosarcoma Saos-2 cells. J. Cell Sci., 112, 631-640 (1999)... 

Zini, N. Ognibene, A. Bavelloni, A. Santi, S. Sabatelli, P. Baldini, N. Scotland , K. Serra, M. Maraldi, N.M. Cytoplasmic and nuclear localization sites of phosphatidylinositol 3-kinase in human osteosarcoma sensitive and multidrug-resistant Saos-2 cells. Histochem. Cell Biol., 106, 457-464 (1996)... 

Gillette, J.M., Chan, D.C., and S.M. Nielsen-Preiss, 2004, Annexin 2 expression is reduced in human osteosarcoma metastases. J Cell Biochem. 92(4) 820—32. 

Luu HH, Zhou L, Haydon RC, Deyrup AT, Montag AG, Huo D, Heck R, Heizmann CW, Peabody TD, Simon MA, He TC. 2005. Increased expression of S100A6 is associated with decreased metastasis and inhibition of cell migration and anchorage independent growth in human osteosarcoma. Cancer Lett 229(1) 135—148. 

Krueger S, Haeckel C, Buehling F, Roessner A (1999) Inhibitory effects of antisense cathepsin B cDNA transfection on invasion and motility in a human osteosarcoma cell line. Cancer Res 59 6010-6014... 

Levemir) [6,20] Insulin aspart (NovoLog, NovoRapid) analogue 12 month (dog) in vitro mitogenic potential (CHO-K1, MCF-7, human osteosarcoma, L6-hIR) No Although mammary tumors were observed battery EMEA-2004... 

The human osteosarcoma cell line has been shown selectively to express COX-2 by reverse transcription-polymerase chain reaction and immunoblot analysis, whereas undifferentiated human lymphoma U937 cells... 

Wong E, DeLucca C, Boily C et al. (1997) Characterization of autocrine inducible prostaglandin H synthase-2 (PGHS-2) in human osteosarcoma cells. Inflamm Res 46 51-59... 

TPA responsive element (TRE)] and increases their expression. FOS is overexpressed in the majority of human osteosarcomas, while JUN is overexpressed in some lung cancers. In Burkitt s lymphoma, the transcription factor MYC is translocated and inserted near an immunoglobulin locus, where it falls under the regulation of an immunoglobulin promoter (Figure 24.18). This results in constitutive expression of high levels of MYC. In addition, various members of the MYC family are amplified in a variety of human and rodent tumors. 

Franceschi RT, Romano PR, and Park KY (1988) Regulation of type I collagen synthesis by 1,25-dihydroxyvitamin D3 in human osteosarcoma cells. Journal of Biological Chemistry 263,18938-45. 

Berlin, O., Samid, D., Donthineni-Rao, R., Akeson, W., Amiel, D. and Woods, V. L. (1993). Development of a novel spontaneous metastasis model of human osteosarcoma transplanted orthotopically into bone of athymic nude mice. Cancer Res. S3,4890-4895. 

CmaUc, S., Hakansson, L, Boquist, L., Lofvenberg, R. and Brostrbm, L. A. (1997). A novel spontaneous metastasis model of human osteosarcoma developed using orthotopic transplantation of intact tumor tissue into tibia of nude mice. Clin. Exptl. Metastasis 15, 164-172. 

Wiedlocha A, Falnes P0, Rapak A, Munoz R, Klingenberg O, Olsnes S (1996) Stimulation of proliferation of a human osteosarcoma cell line by exogenous acidic fibroblast growth factor requires both activation of receptor tyrosine kinase and growth factor internalization. Mol Cell Biol 16 270-280. 

Farley JR, Kyeyune-Nyombi E, Tarbaux NM, HaU SL, Strong DD. Alkaline phosphatase activity from human osteosarcoma cell line SaOS-2 an isoenzyme standard for quantifying skeletal alkaline phosphatase activity in serum. Clin Chem 1989 35 223-9. 

Culture Human osteosarcoma Saos-2 cells (ATCC HTB-85) in seven flask T75 (around 25,000 cells/cm ) with 15 mL mineralization medium for Saos-2 cells see Subheading 2.2) for each flask. 

Oda Y, Yamamoto H, Tamiya S, et al. CXCR4 and VEGF expression in the primary site and the metastatic site of human osteosarcoma analysis within a group of patients, all of whom developed lung metastasis. Mod Pathol. 2006 19 738-745. 

Ferguson RJ, Tunis EJ. The ultrastructure of human osteosarcoma a study of nine cases. Clin Orthop. 1978 234-246. 

Varelias A, Koblar SA, Cowled PA, et al. Human osteosarcoma expresses specific ephrin profiles implications for tumorigenic-ity and prognosis. Cancer. 2002 95 862-869. 

Trieb K, Lehner R, Stulnig T, et al. Survivin expression in human osteosarcoma is a marker for survival. Eur J Surg Oncol. 2003 29 379-382. 

Haydon RC, Deyrup A, Ishikawa A, et al. Cytoplasmic and/or nuclear accumulation of the beta-catenin protein is a frequent event in human osteosarcoma. Int J Cancer. 2002 102 338-342. 

Such experiments were performed in the p53-proficient human osteosarcoma cell line U20S using the gap-lesion plasmid with a site-specific cisplatin-GG [38]. 

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