CDNA transfectants

It is also important to predict the in vivo biliary excretion clearance in humans, and for this purpose MDCK II cell lines expressing both uptake and efflux transporters may be used (Fig. 12.3) [92, 93]. It has been shown that MRP2 is expressed on the apical membrane, whereas OATP2 and 8 are expressed on the basolateral membrane after cDNA transfection (Fig. 12.3) [92, 93]. The transcellular transport across such double-transfected cells may correspond to the excretion of ligands from blood into bile across hepatocytes. Indeed, the vectorial transport from the basal to apical side was observed for pravastatin only in OATP2- and MRP2-expressing... 

Ishizuka, T., Murata, N., Kanda, T., Kobayashi, I., Ohta, H., Ui, M. and Okajima, F., 1999, Comparison of intrinsic activities of the putative sphingosine 1-phosphate receptor sub-types to regulate several signaling pathways in their cDNA-transfected Chinese hamster ovary cells. J. Biol. Chem. 274 23940-23947. 

Mochizuki, O. N., Nakajima, Y., Nakanishi, S., Ito, S. Characterization of the substance P receptor-mediated calcium influx in cDNA transfected Chinese hamster ovary cells. A possible role of inositol 1,4,5-triphosphate in calcium influx, J. Biol. Chem. 1994, 269, 9651-9658. 

Shi, F.-S., Weber, S., Gan, J.,Rakhmilevich, A.L. andMahvi,D.M. (1999)GM-CSF secreted by cDNA transfected tumor cells induces a more potent antitumor response than exogenous GM-CSF. Cancer Gene Then, 6, 81-88. 

Tohda M, Tohda C, Oda H, Nomura Y. Possible involvement of botulinum ADP-ribosyltransferase sensitive low molecular G protein on 5-hydroxytryptamine (5-HT)-induced inositol phosphates formation in 5-HT2c cDNA transfected cells. Neurosci Lett 1995 190 33-36. 

Krueger S, Haeckel C, Buehling F, Roessner A (1999) Inhibitory effects of antisense cathepsin B cDNA transfection on invasion and motility in a human osteosarcoma cell line. Cancer Res 59 6010-6014... 

Gorelik, E., Xu, R, Henion, T., Anaraki, R and Galili, U. (1997). Reduction of metastatic properties of BL6 melanoma cells expressing terminal fucose(alpha) 1-2-galactose after alpha 1,2-fucosyltransferase cDNA transfection. Cancer Res. 57, 332-336. 

Fogelman SM, Schmider J, Venkatakrishnan K, von Moltice LL, Harmatz JS, Shader RI, Greenblatt DJ. O- and V-dem ethylation of venlafaxine in vitro by human Uver microsomes and by microsomes from cDNA-transfected cells effect of metabolic inhibitors and SSRI antidepressants. Neuropsychopharmacology (1999) 20,480-90. 

Albers K, Fuchs E (1987) The expression of mutant epidermal keratin cDNAs transfected in simple epithelial and squamous cell carcinoma lines. J Cell Biol 105(2) 791-806... 

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