SaOS-2 cell line

Recently, Choy et al. also reported that LDHs are an efficient drug reservoir for folate derivatives [187]. Folic acid derivatives, folinic acid and methotrexate (MTX), have been successfully hybridized with Mg/Al LDHs by ion-exchange reactions. Cellular uptake tests with the MTX-LDH hybrids were carried out in the fibroblast (human tendon) and osteosarcoma (SaOS-2) cell lines by in vitro assay. They found that the LDH not only plays a role as a biocompatible delivery matrix for drugs but also facilitates a significant increase in the delivery efficiency. 

Primary human osteoblast (SAOS-2 cell line) incubated for 1 and 3 weeks Human osteoblastlike MG63 cells on the composite scaffolds... 

In a study of six mercury compounds, mercury chloride, mercury nitrate, sodium ethylmercurithi-osalicylate, methyl mercury chloride, mercury acetate and phenylmercury acetate in MDCK cells, LLC-PKl cells and human primary proximal tubular cells (hPTC) and non-renal cell lines (SAOS and Hep G2) it was found that all mercury compounds were toxic to all cell types as evidenced by neutral red uptake, thymidine incorporation and the MTT assay [189]. However, sodium ethylmercurithiosalicylate, methyl mercury chloride and phenylmercury acetate were one order of magnitude more toxic than the other compounds. In addition the GSH synthesis inhibitor L-buthionine sulfoximine (BSO) potentiated the toxicity of all mercury compounds [189]. In a study using primary rabbit proximal tubular cells it was also shown that methyl mercury chloride is more toxic than mercury chloride [190]. Differences in the extent and rate of metal uptake were also evident. Maximum cellular uptake of Hg " occurred within 6-24 hr after exposure and was not concentration-dependent, whereas maximum uptake of CHgHg" occurred within 3 hr of exposure and was concentration- dependent [190]. 

Some methods have used a combination of techniques to improve specificity. These classic measurements for BAP are usually technically complicated and labor-intensive, imprecise, insensitive, and inaccurate. Of these techniques, heat denaturation and WGA precipitation have been used most frequently. Heat denaturation has been criticized because of its irreprodudbility or variability. WGA precipitation has been criticized because WGA does not precipitate all BAP from pagetic sera or from tire SaOS-2 osteosarcoma cell line and fails to completely separate bone and liver activity. ... 

Farley JR, Kyeyune-Nyombi E, Tarbaux NM, HaU SL, Strong DD. Alkaline phosphatase activity from human osteosarcoma cell line SaOS-2 an isoenzyme standard for quantifying skeletal alkaline phosphatase activity in serum. Clin Chem 1989 35 223-9. 

Urban levels of air pollution (downtown Sao Paulo) modified the progression of urethane (3 g/ kg)-induced lung tumours in mice (Cury etal. 2000). Urban particles consist of three modes ul-trafine particles, accumulation mode particles (which together form the fine particle mode) and coarse mode particles. Ultrafine particles (those of <100nm diameter) contribute very little to the overall mass, but are very high in number, which in episodic events can reach several hundred thou-sand/cm in the urban air. The hypothesis that ultrafine particles are causally involved in adverse responses seen in sensitive humans is based on several studies summarised by OberdOrster (2001). Timblin etal. (2002) demonstrated the development of dose-related proliferation and apoptosis after exposure of an alveolar epithelial cell line (CIO) to particulate matter or to ultrafine carbon black, a component of particulate matter. Ribonuclease protection assays demonstrated that increases in mRNA levels of the early response protooncogenes c-jun, junB, fra-l, and/ra-2 accompanied cell pro-hferation at low concentrations of particulate matter whereas apoptotic concentrations of particulate... 

Allen et al. (1994) also reported that DLCs interact well with human osteoblast-like cell line SaOS-2. When they compared the growth of human osteoblasts in both the DLC-coated and uncoated polystyrene plates, they found a similar level of growth observed in both samples, and the osteoblasts adhered well to the DLC samples and produced extensive filopodia when viewed under the scanning electron microscope (AUen et al., 1998). The LDH assay of the osteoblast-like cells also indicated that DLC caused no significant level of cell lysis or toxicity compared with the uncoated samples (Allen et al., 1994). 

It is desirable to show how new platinum structures may differ in signalling pathways from those of cisplatin and oxaliplatin. An important example is the role of pS3, the tumor suppresor g te, in modulation of cytotoxicity of platinum drugs. BBR 3464 di lays high activity in human tumor cell lines characterized by both wild type and mutant pS3 gene (21). In contrast, on average, cells with mutant pS3 are more resistant to the effea of cisplatin (25). It has been hypothesized that sensitivity or resistance of tumor cells to cisplatin might be also associated widi the processes involving pS3 (26,27). Transfer of functional pS3 into p53-null SAOS osteosarcoma cells actually reduced cellular sensitivity... 

BC is a good material not only for wound treatment and other fields of veterinary medicine, but also as a scaffold material for cell cultivation in tissue engineering [156,157]. On such scaffolds the fzmb has cultivated the following cell types successfully human osteoblasts, human osteogenic sarcoma cells (SAOS-2), equine osteoblast lines and chondrocytes, and mesenchymal stem cells. 

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