Fluorescent studies

Sonnenfroh D M and Leone S R 1989 A laser-induced fluorescence study of product rotational state distributions in the charge transfer reaction Ar <-i. i, ) + Ni Ar + MfXjat 0.28 and 0.40 eV J. them. Phys. 90 1677-85... 

Duncan M A, Bierbaum V M, Ellison G B and Leone S R 1983 Laser-induced fluorescence studies of ion collisional excitation in a drift field rotational excitation of N in He J. Chem. Phys. 79 5448-56... 

Reviews fast transient kinetic studies of bioiogicai moiecuies (exciuding fluorescence studies). 

Ultrafiltration utilizes membrane filters with small pore sizes ranging from O.OlS t to in order to collect small particles, to separate small particle sizes, or to obtain particle-free solutions for a variety of applications. Membrane filters are characterized by a smallness and uniformity of pore size difficult to achieve with cellulosic filters. They are further characterized by thinness, strength, flexibility, low absorption and adsorption, and a flat surface texture. These properties are useful for a variety of analytical procedures. In the analytical laboratory, ultrafiltration is especially useful for gravimetric analysis, optical microscopy, and X-ray fluorescence studies. 

Lee, J., O Kane, D. J., and Gibson, B. G. (1989). Dynamic fluorescence study of the interaction of lumazine protein with bacterial luciferases. Biophys. Chem. 33 99-111. 

Visser, A. J. W. G., and Lee, J. (1980). Lumazine protein from the bioluminescent bacterium Photobacterium phosphoreum. A fluorescence study of the protein-ligand equilibrium. Biochemistry 19 4366-4372. 

OPCp CD measurements in different solvents at different pH, fluorescence studies after labeling, melting properties... 

The most commonly used dye in fluorescence studies on nucleic acids is ethidium bromide. The dye has broad excitation bands centered around 280 and 460 nm and a strong emission around 600 nm. When the dye hinds to DNA by an intercalative mechanism, its emission is greatly enhanced and slightly shifted in wavelength. In the simplest case with ethidium bromide saturating intercalating sites,... 

To obtain an increased intrinsic capacity to transgress biological membranes, a number of different modifications have been introduced to PNA. These modifications include conjugation of PNA to Hpophilic moieties [51, 97, 98], conjugation of PNA to certain so-caUed ceU-penetrating peptides [49, 55, 56, 66, 99-102] and conjugation to different moieties, which are supposed to be internahzed by specific cellular receptors [48, 103-105]. The work on cellular dehvery of PNA is, like the related work on ex vivo and in vivo effects of PNA, very difficult to summarize conclusively. First of all, the pronounced diversity of the reporter systems employed makes it impossible to directly compare the studies. Secondly, the widespread use of fluorescence studies in spite of the many inherent pitfalls of this technique makes it sometimes difficult to judge even qualitatively whether a presented result actually indicates cellular uptake. We have recently published a comprehensive review on cellular dehvery of PNA [82], with a more detailed assessment of the PNA dehvery hterature. 

In 1999, the Klemperer group published experimental results from a dispersed fluorescence study of the linear and T-shaped Ar l2(X, v = 0) conformers to obtain information about the X- and B-state potentials of the complex and to gain insight into the photodissociation dynamics in the B electronic state [65]. In carrying out their analysis, they assumed that the linear and T-shaped conformers existed in a thermodynamic equilibrium, with the abundance of the linear conformer being three times that of the T-shaped conformer. In an effort to test the validity of the thermodynamic hypothesis Bastida, et al. [66], performed a... 

Grubb RL, Jr., Phelps ME, Ter-Pogossian MM. Regional cerebral blood volume in humans. X-ray fluorescence studies. Arch Neurol 1973 28 38-44. 

Itoh, M. Adachi, T. Transient absorption and two-step laser excitation fluorescence studies of the excited-state proton transfer and relaxation in the methanol solution of 7-hydroxyflavone. J. Am. Chem. Soc. 1984, 106, 4320 -324. 

Itoh, M. Hasegawa, K. Fujiwara, Y. Two-step laser excitation fluorescence study of the ground- and excited-state proton transfer in alcohol solutions of 7-hydroxyisoflavone. J. Am. Chem. Soc. 1986, 108, 5853-5857. 

Mullineaux, C.W., Pascal, A.A., Horton, P. and Holzwarth, A.R. 1992. Excitation energy quenching in aggregates of the LHCII chlorophyll-protein complex A time-resolved fluorescence study. Biochim. Biophys. Acta 1141 23-28. 

Chakraborty, S., Ittah, V., Bai, P., Luo, L., Haas, E., and Peng, Z. Y (2001). Structure and dynamics of the alpha-lactalbumin molten globule Fluorescence studies using proteins containing a single tryptophan residue. Biochemistry 40, 7228-7238. 

Fluorescence Studies. Fluorescence spectra of films on glass plates were obtained with a Perkin-Elmer MPF-3 spectro-fluorimeter. A previously-described phase fluorimeter was utilized for fluorescence lifetime determinations. 

Ellison EH, Moodley D, Hime J (2006) Fluorescence study of arene probe microenvironment in the intraparticle void volume of zeolites interfaced with bathing polar solvents. J Phys Chem B 110 4772 1781... 

Fluorescence studies and the binding interaction of Quartz/APES/RB with single- and double-stranded oligonucleotides (ssDNA and dsDNA) in Tris-HCl buffer solution of pH 7.4 were carried out. Quartz/APES/RB exhibited emission at 576 nm, whereas Quartz/APES without BB where nonfluorescent, suggesting that RB successfully assembled on the surface of quartz wafers. By comparison with Aem of 5 x 10 5 M RB solution, which was 588 nm, a hypsochromic shift was found. Considering the fluorescence microscopical image of Quartz/APES/RB, it... 

Ioffe VM, Gorbenko GP, Deligeorgiev T, Gadjev N, Vasilev A (2007) Fluorescence study of protein - lipid complexes with a new symmetric squarylium probe. Biophys Chem... 

Jang DJ, Kelley DF (1985) Time-resolved and steady-state fluorescence studies of the excited-state intramolecular proton transfer and relaxation of 2-hydroxy-4, 5-naphthotro-pone. J Phys Chem 89 209-211... 

Shynkar VV, Mely Y, Duportail G et al (2003) Picosecond time-resolved fluorescence studies are consistent with reversible excited-state intramolecular proton transfer in 4 -(dialkylamino)-3-hydroxyflavones. J Phys Chem A 107 9522-9529... 

Lapouyade R, Czeschka K, Majenz W, Rettig W, Gilabert E, Rulliere C (1992) Photophysics of donor-acceptor substituted stilbenes. A time-resolved fluorescence study using selectively bridged dimethylamino cyano model compounds. J Phys Chem 96(24) 9643-9650... 

Sakura, S. and Fujimoto, D. (1984) Absorbtion and fluorescence studies of tyrosine derived crosslinking amino acids from collagen. Photochemistry and Photobiology 40, 731-734. 

In spite of the heavy atom, compound (32) is sufficiently fluorescent for this to be used as an analytical tool to examine localization and pharmacokinetics. In EMT-6 murine tumors, (32) localizes initially on lysosomes, with selectivity for tumor over surrounding normal tissue, and with evidence for apoptotic cell kill.137 Fluorescence studies using a hamster cheek pouch model show a maximum emission in 2-3 h, with selectivity for the tumor (x 1.5 over normal tissue) after 24 h the photosensitizer is no longer detectable.138 Lutetium texaphyrin (32) has been compared... 

Bastiaens, P. I. H., van Hoek, A., Benen, J. A., Brochon, J. C. and Visser, A. J. W. G. (1992). Conformational dynamics and intersubunit energy transfer in wild-type and mutant lipoamide dehydrogenase from Azotobacter vinelandii. A multidimensional time-resolved polarized fluorescence study. Biophys. J. 63, 839-53. 

In the case of 7-methyl-B[a]A (155) and 7,12-dimethyl-B(a]A (DMBA) (50,55,57,156-158) most of the evidence supports bay region diol epoxide adducts. Thus, fluorescence studies (50,55,56,159), light sensitivity of the adducts (160), and analysis of adducts formed by further oxidations of the 3,4-dihydrodiol all suggest a bay region diol epoxide intermediate (161,162). More recent evidence... 

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