Fluorescence Spectroscopic Studies

Fluorescence spectroscopy has several useful applications in studying the binding site, and it has the significant advantage that it is 

The 2-(m-aminobenzoyl)taxol derivative 11.3.8 gave interesting results, since it was found that its absorption and emission spectra, and specifically the difference between them, were solvent dependent. Using this information it was possible to ascertain that the fluorophore binding site on the microtubule is in an environment of intermediate polarity, and it was also shown that tubulin has two binding sites for taxol, one high affinity site and one low affinity site (397). 

A later paper from the same group describes results with the 3 -A-(m-aminobenzoyl)taxol 11.3.2 (395). It was found that 11.3.2 bound to two types of site on microtubules with binding affinities of 61 nM and 3.3 iM. It bound to a single site on microtubules assembled from GDP-tubulin with a dissociation constant of 2.5 pM, and it bound to a single site on microtubules assembled from the GTP analog GMPCPP with a dissociation constant of 15 nM. It was thus proposed that although all the subunits of the microtubule at the steady state are the same GTP- 

A recent paper describes the use of Flutax-1 and -2 (11.3.12 and 11.3.13) as probes for the study of the binding of taxol to microtubules. A binding mechanism consisting of a fast bimolecular reaction followed by at least two slow monomolecular rearrangements was developed. The results also suggested that the taxol binding site is directly accessible, in opposition to some other models of microtubules (470). 

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From the results of the fluorescence spectroscopic study it is concluded that excitation energy at the lowest excited state of a PDA derivative having a thioester moiety is localized at the thioester group intra- or inter-molecular energy transfer from the conjugated system of the PDA to the thioester moiety must have occurred in the crystalline state to afford a photostable crystal (Hasegawa et al., unpublished data). 

Vaz, W. L. C. and Melo, E. (2001) Fluorescence spectroscopic studies on phase heterogeneity in lipid bilayer membranes./. Fluoresc., 11, 255-271. 

In addition, data obtained from infrared, thermal, and fluorescence spectroscopic studies of the outermost layer of skin, stratum corneum (SC), and its components imply enhancer-improved permeation of solutes through the SC is associated with alterations involving the hydrocarbon chains of the SC lipid components. Data obtained from electron microscopy and x-ray diffraction reveals that the disordering of the lamellar packing is also an important mechanism for increased permeation of drugs induced by penetration enhancers (for a recent review, see Ref. 206). 

Kim, H.-B., Habuchi, S., and Kitamura, N., Dynamic fluorescence spectroscopic study on the microstructures in ion-exchange resin particles, Anal. Chem., 71, 842, 1999. 

Wu, C.-H., I. Salmeen, and H. Niki, Fluorescence Spectroscopic Study of Reactions between Gaseous Ozone and Surface-Adsorbed Polycyclic Aromatic Hydrocarbons, Environ. Sci. Tech-noL, 18, 603-607 (1984). 

Chen, I, LeBoeuf, E. I, Dai, S., and Gu, B. (2003). Fluorescence spectroscopic studies of natural organic matter fractions. Chemosphere 50, 639-647. 

Kim, H., Ueno, K., Chiba, M., Kogi, O., Nobom, K., Spatially-resolved fluorescence spectroscopic study on tiquid/tiquid extraction processes in polymer microchannels. Anal. Sci. 2000, 16, 871. 

Deepa, S., Mishra, A.K. (2005). Fluorescence spectroscopic study of serum albumin-bromadiolone interaction fluorimeteric determination of bromadiolone. J. Pharm. Biomed. Anal. 38(3) 556-63. 

Doppler-free and Doppler-limited fluorescence spectroscopic studies of the He Is2p-ls3d transitions Calculation of electron excitation rate constants for formation of He(ls2s, - S) and He(ls2p, - p ) using evaluated experimental and theoretical data... 

Photocycloreversions have been reported in 1,2,3,4-tetraphenylcyclo-butane and in the endoperoxide of 1,4,9,10-tetraphenylanthracene, and fluorescence spectroscopic studies have shown that cycloreversion of cis- and trtms-acenaphthylene dimers (178) to acenaphthylene proceeds by way of the excited singlet state. 

Fluorescence Spectroscopic Studies of Al—Fulvic Acid Complexation in Acidic... 

KK Pandey, NK Upreti, and VV Srinivasan. A Fluorescence Spectroscopic Study on Wood. WoodSci. Technol. 32 309-315, 1998. 

Phillips D, Roberts AJ, and Soutar I. A time-resolved fluorescence spectroscopic study of excimer formation in polyacenaphthylene and an alternating acenaphthylene/maleic anhydride copolymer. J. Polym. Sci. Polym. Lett. 1980 18 123-129. 

A.M. Ellis, E.S.J. Robles, and T.A. Miller, Dispersed Fluorescence Spectroscopic Study of the Ground Electronic State of Silver Trimer , Chem. Phys. Lett. 201, 132 (1993). 

Chakraborty, A., Seth, D., Chakrabarty, D., Setua, R, and Sarkar, N. 2005. Dynamics of solvent and rotational relaxation of Coumarin 153 in room-temperature ionic liquid l-butyl-3-methylimidazolium hexafluorophosphate confined in brij-35 micelles A picosecond time-resolved fluorescence spectroscopic study. J. Phys. Chem. A 109,11110-11116. 

Through coor nation motif, a tetra-pyrazinyl porphyrin-based assembly with four dimeric [meso-tetrakis(2-carboxy-4-nonylphenyl)porphyrinato]-zinc(II) as the antenna moiety was constructed by Kuroda et al. [113]. The efficiency of energy transfer from the zinc porphyrin-pyrazine complex to the free base porphyrin has been determined to be 82%. Also for this system, the observed efficiency of energy transfer is not so high compared with those reported for covalently finked multi-porphyrin systems [114]. Himter s group has designed a pentameric porphyrin array, wtiich was built up by the complementary coordination of a tetra-pyridyl porphyrin and two zinc-porphyrin dimers [115]. Steady-state and time-resolved fluorescence spectroscopic studies revealed that the energy transfer efficiency of this array is around 70%. 

M. Yanagimachi, N. Tamai, and H. Masuhara, Chem. Phys. Lett., 200, 469 (1992). Solvation Dynamics of a Coumarin Dye at Liquid Solid Interface Layer - Picosecond Total Internal-Reflection Fluorescence Spectroscopic Study. 

H. M. Kwaambwa and R. Maikokera, A fluorescence spectroscopic study of a coagulating protein extracted from Moringa oleifera seeds, Colloids and Surfaces B, vol. 60, no. 2, pp. 213-220, 2007. 

Murari, B. M. Anand, S. Gohil, N. K. Chaudhury, N. K. Fluorescence spectroscopic study of dip coated sol-gel thin film internal environment using fluorescent probes Hoechst33258 and Pyranine. J. Sol-Gel Sci. Technol. 2007,41,147-155. 

Lin, T. L Dowben, R. M. Fluorescence spectroscopic studies of pyrene-actin adducts. Biophys. Chem. 1982,15, 289-298. 

Rao, B., Kemple, M., and Prendergast, E, Proton nuclear magnetic resonance and fluorescence spectroscopic studies of segmental mobility in aequorin and green fluorescent protein, Photochem. Photobiol, 51, 92,1980. 

Mishra, B., Barik, A., Priyadarsini, I., and Mohan, H. (2005). Fluorescent spectroscopic studies on binding of a flavonoid antioxidant quercitin to serum albumins. J. Chem. Soc., 117, 641-647. 

Ryan, D.K., Shia, C.R, and Oconnor, D.V. (1996). Fluorescence spectroscopic studies of Al-fulvic acid complexation in acidic solutions. Humic Fulv. Acids Isolat., Struct., Environ. Role, 651,125-139. 

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