Fluorescent in situ hybridization studies

Bishit and Mukai (2000,2001 a,b), in a series of FISH (fluorescent in situ hybridization) studies, reported that there were three diploid species. The two tetraploid species . africana and . coracana are related to one another. They also suggested that . multiflora was a distinct species and that the two diploid species . indica and . floccifolia are probably the donors to the tetraploid species. This conclusion was based on the pattern of hybridization of a 5sRNA probe of the chromosomes of the species that they studied. However, phylogenetic analysis of sequence data does not appear to support this hypothesis none of the ITS found in E. coracana closely related to the ITS of E. floccifolia (Neves et al. 2005). 

Immunohistochemical studies demonstrate overexpression of HER-2 protein in non-small-cell lung carcinoma (NSCLC). The protein is located predominantly on the plasma membrane, although its location has been reported in the cytoplasm, especially in adenocarcinoma. In a recent immunohistochemical and fluorescence in situ hybridization study, with the exception of occasional single tumor cells, cytoplasmic HER-2 expression was not seen (Cox et al., 2001), in contrast to some other studies employing different antibodies and other processing parameters (Tateishi et al., 1991). 

This seems to be the first case of drng-related acnte myelogenous leukemia. Fluorescent in-situ hybridization studies on the bone marrow showed a signal consistent with rearrangement of the mixed myeloid leukemia (MLL) gene on chromosome 11. This abnormality was not present before treatment. 

Mielke, V., Bauman, J.G., Sticherling, M., lbs, T., Zomershoe, A.G., Seligmann, K., Henneicke, H.H., Schroder, J.M., Sterry, W. and Christophers, E. (1990). Detection of neutrophil-activating peptide NAP/IL-8 and NAP/IL-8 mRNA in human recombinant IH alpha- and human recombinant tumor necrosis factor-alpha-stimulated human dermal fibroblasts. An immunocytochemical and fluorescent in situ hybridization study. J. Immunol. 144, 153-161. 

Paner GP, Lindgren V, Jacobson K, et al. High incidence of chromosome 1 abnormalities in a series of 27 renal oncocytomas cytogenetic and fluorescence in situ hybridization studies. Arch Pathol Lab Med. 2007 31 81. 

Sugimura H. Detection of chromosome changes in pathology archives an application of microwave-assisted fluorescence in situ hybridization to human carcinogenesis studies. Carcinogenesis 2008 29 681-687. 

Trask, B.J., Allen, S., Massa, H., Fertitta, A., Sachs, R., van den Engh, G., and Wu, M. (1993) Studies of metaphase and interphase chromosomes using fluorescence in situ hybridization. Cold Spring Harb. Symp. Quant. Biol. 58, 767-775. 

Lacroix-Triki M, Mounie E, Charafe-Jauffret E, et al. Double staining chromogenic in situ hybridization is a useful alternative to fluorescent in situ hybridization first comparative study of HER2 and TOP2A gene amplification in breast cancer. Proc. San. Antonio Breast Cancer Symp. 2007. 

Controversy exists as to the most useful assay for HER2-overexpres-sion, particularly for identification of candidates for trastuzumab therapy. Following the approval of trastuzumab, an IHC-based assay (Herceptest), similar but not identical to the trastuzumab clinical trials assay, was developed for this use. Fluorescence in situ hybridization (FISH)-based assays have also been developed to determine HER2 gene amplification. The concordance of these assays with each other and with the trastuzumab clinical trials assay has been questioned (252). Since the probability of response to trastuzumab therapy appears to correlate with the level of HER2 overexpression, further studies will be required to establish standardized HER2 testing procedures. 

Tuohy, K.M., Finlay, R.K., Wynne, A.G., and Gibson, G.R., A human volunteer study on the prebiotic effects of HP-inulin-faecal bacteria enumerated using fluorescent in situ hybridization (FISH), Ecol. Environ. Microbiol., 7, 113-118, 2001. 

The above studies established a precedent for the combined use of microbiological, genomic, and isotopic methods to study important biogeochemical processes. Eurthering the revolution of culture-independent methods for studying these processes on natural samples has been the visualization of the active microbes through fluorescence in situ hybridization (Boetius et al., 2000),... 

Sun B, Sun Y, Wang J, et al. The diagnostic value of SYT-SSX detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and fluorescence in-situ hybridization (FISH) fot synovial sarcoma A review and prospective study of 255 cases. Cancer Sci. 2008 99 1355-1361. 

Studies for DNA ploidy, including computer-assisted image analysis of Feulgen stains, and cytogenetics, including fluorescent in situ hybridization (FISH), have... 

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