Fluorescence studies on nyctinasty

The mechanisms by which leaf-movement factors induce leaf movement have been examined by using molecular probes consisting of chemically modified leaf-movement factors designed to identify the target cells of these factors. Structure-activity relationships for such probes were explored (Fig. 8.6 Ueda et al, 1999b). In this chapter, we focus on the phenolic leaf-opening factor, potassium isolespedezate (13). 

2 Photoaffinity labeling of the target protein for leaf-movement factor 

Photoaffinity labeling (Nakayama et al, 1991 Hatanaka et al, 1994a,b, 1996 Kotzyba-Hilbert et al, 1995 Hashimoto et al, 1997, 2001 Curry et al, 1998 Koumanov et al, 1998 Fishkin et al, 2000 Matsubayashi et al, 2000) probes based on potassium isoles-pedezate (13) have been developed to explore the target protein for 13, the leaf-opening 

The target cell of leaf-movement factor is motor cell  

3 Are leaf-movement target proteins common to the same 

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Nagano, H., Kato, E., Yamamura, S. Ueda, M. (2003). Fluorescence studies on nyctinasty which suggest the existence of genus-specific receptors for leaf-movement factor. Organic Biomolecular Chemistry, 1, 3186-3192. 

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