Protein conjugates, fluorescence polarization studies

HPhe polarization of fluorescence technique employing dye-macromolecule x conjugates is a sensitive hydrodynamic method for studying the structure and interactions of proteins 19, 20, 41, 54, 65) and synthetic polypeptides 26, 30, 31, 49)- The relationship describing the dependence of polarization of fluorescence upon the Brownian rotational diffusion of the macromolecule was developed by Perrin 50) and extended by Weber 65) in the form of the equations (for excitation with natural light) ... 

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