Electron microscope analysis

The biflagellate unicellular green alga Chlamydomonas reinhardtii is prone to spontaneous mutations that produce deficiencies in flagellar proteins and MT assembly, substructure, and function. Viable mutants that are either nonmotile or slow moving can be isolated and analyzed biochemically and morphologically, thereby establishing structure-function correlations. Electron microscopic analysis... 

Epoxidized oils were also used to modify PLA Ali et ah (2009) reported that its use as a plasticizer to improve flexibility. Thermal and scanning electron microscope analysis revealed that epoxidized soybean oil is partially miscible with PLA. Rheological and mechanical properties of PLA/epoxidized soybean oil blends were studied by Xu and Qu (2009) Epoxidized soybean oil exhibited a positive effect on both the elongation at break and melt rheology. Al-Mulla et al. (2010b) also reported that plasticization of PLA (epoxidized palm oil) was carried out via solution casting process using chloroform as a solvent. The results indicated that improved flexibility could be achieved by incorporation of epoxidized palm oil. 

The large size of CPOs allows their direct observation. For this purpose, scanning tunneling microscopy (STM) is the best method [32,34]. Electron microscopic analysis is used for phthalocyanine 3 and its derivatives however, most of the porphyrin derivatives are decomposed by electron beam irradiation. Presently, although only a limited number of researchers are able to perform atomic-scale resolution measurement, this powerful analytical method is expected to be used widely in the future. The author reported a summary of STM studies on porphyrins elsewhere [34]. 

Henderson, W.J. and Barr, W.T. (1988). Specific area Icxation, isolation and recovery from paraflSn sections for electron microscope analysis. J. Histotechnol. 11, 43-46. 

For this purpose, all three catalyst supports were initially synthesized by a chemical vapor deposition (CVD) process and thereafter, using a wet impregnation method, loaded with cobalt as the active component for FTS. The as-synthesized Co/nanocatalysts were then characterized by applying electron microscopic analysis as well as temperature-programmed desorption, chemi- and physisorption measurements, thermogravimetric analysis, and inductively coupled plasma... 

Shirahama, T., and Cohen, A. S. (1967). High-resolution electron microscopic analysis of the amyloid fibril./ Cell Biol. 33, 679-708. 

The CSN is composed of eight subunits called CSNl to CSN8, which are highly conserved in eukaryotes, although only six of them occur in fission yeast. Two hybrid screens and biochemical methods such as far westerns, pull downs and coprecipitation defined a number of CSN subunit-subunit interactions. Figure 13.1 illustrates known subunit-subunit interactions. Initial insight into the architecture of CSN came from the first 2D electron microscopic analysis of purified CSN from human red blood cells [19] (see also Figure 13.2 below). 

UPD process has also been studied on screen-printed silver electrodes using voltammetric techniques and scanning electron microscope analysis [293]. The relative occurrence of UPD and bulk Pb process has been dependent on the scan rate, with increasing role of UPD process in higher rates. Studies on Pb deposition on silver colloids have pointed to its similarity to bulk electrode [283]. 

Indeed, at very high polymer concentrations enhancement of these effects occurs. Recent studies by Franks et al. 23) on the rheological behaviour and freeze fracture electron microscopical analysis of several synthetic linear flexible polymers, including poly(vinylpyrrolidone) and polyethylene glycol) in concentrated solutions, suggest that these molecules do not form a network mesh but rather exhibit aggregation. Anionic polysaccharides, on the other hand, are known to form an anisotropic packing array in condensed films. These films may be stretched to enhance orientation and be used for X-ray diffraction studies... 

Chytrid hydrogenosomes took rather different from the pictures of mitochondria in textbooks (Fig. 4). However, serial sectioning followed by electron microscopical analysis revealed a structure resembling the ultrastructure... 

Trichohyalin contains extensive repeat sequence blocks, which are predicted to form an elongated, flexible single-stranded o-helical rod (Lee et al, 1993). These predictions are consistent with biochemical and electron microscopical analysis showing an extended shape with overall dimensions of 85 nm. Similar to profilaggrin, the N-terminus comprises an S-100 protein-like calcium-binding domain. 

Frey, P. A. and Frey, T. G. (1999) Synthesis of undecagold labeling compounds and their applications in electron microscopic analysis of multiprotein complexes. J. Struct. Biol. 127, 94-100. 

Grewe PH, Deneke T, Holt SK, et al. Scanning electron microscopic analysis of vessel wall reactions after coronary stenting. Z Kardiol 2000 89(l) 2l-27. 

Jankowski MP, Sesack SR. Electron microscopic analysis of the GABA projection from the dorsal raphe nucleus to the prefrontal cortex in the rat. Soc Neurosci Abstr 587.8. 

Hersch SM, Ciliax BJ, Gutekunst CA, Rees HD, Heilman CJ, Yung KKL, Bolam JP, Ince E, Yi H, Levey AI (1995) Electron microscopic analysis of D1 and D2 dopamine receptor proteins in the dorsal striatum and their synaptic relationships with motor corticostriatal afferents. J Neurosci 75 5222-5237. 

Lipozits Zs, Phelix C, Pauli WK (1986) Electron microscopic analysis of tyrosine hydroxylase, dopamine-p-hydroxylase and phenylethanolamine-N-methyltransferase immunoreactive innervation of the hypothalamic paraventricular nucleus. Histochemistry 84 105 120. 

Ultrastructural studies of the D5 dopamine receptor using immunocytochemistry have revealed that this receptor subtype is highly expressed in the human cortex in pyramidal neurons and their dendrites are present within layers IV-VI (Khan et al., 2000). The D5 dopamine receptor is also localized to the striatum, substantia nigra (both pars compacta and reticulata), the superior colliculus, the thalamus and the pyramidal cells of hippocampus (Khan et al., 2000). In the striatum, electron microscopic analysis indicates that D5 dopamine receptors are present in the spines where asymmetric synapses are formed... 

Gilkes, R.J., Transmission electron microscope analysis of soil minerals, in Quantitative Methods in Soil Mineralogy, Amonette, J.E. and Zelazny, L.W., Eds., Soil Science Society of America, Madison, WI, 1994, p. 177. 

Roggli VL, Coin PG, MacIntyre NR, et al. 1994a. Asbestos content of bronchoalveolar lavage fluid A comparison of light and scanning electron microscopic analysis. Acta Cytol 38 502-510. 

Srebro SH, Roggli VL, Samsa GP. 1995. Malignant mesothelioma associated with low pulmonary tissue asbestos burdens A light and scanning electron microscopic analysis of 18 cases. Mod Pathol 8 614-621. 

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