Sectioning serial

Schnitt, m. cut cutting, incision section intersection slice die edge (of a book) (surgical) operation crop, -band, n. (Micros.) serial section, ribbon, schnlttbearbeltbar, a. machinable. Schnitt-brenner, m. slit burner, batswing burner, -dicke, /, thickness of a slice or section. 

Serien-nummer, /. serial number, -schaltung, /. (Elec.) series connection, -schnitt, m. serial section, -spektrum, n. series spectrum, versuch, m. serial experiment or test. Serizin, n. sericin. 

Figure 18. (a) Immunocytochemical labeling of B-lymphocytes in muscle from a patient with juvenile dermatomyositis. (b) Immunocytochemical labeling of T8 lymphocytes showing that the infiltrate contains very few cells of this type (serial section). 

Fig. 20.2 Chronic lithium and valproate robustly increase bcl-2 immunoreactive neurons in the frontal cortex. Male Wistar Kyoto rats were treated with either Li2C03, valproate or saline by twice daily i.p. injections for four weeks. Rats brains were cut at 30 pm serial sections were cut coronally through the anterior portion of the brain, mounted on gelatin-coated glass slides and were stained with thio-nin. The sections of the second and third sets were incubated free-floating for 3 d at 4°C in 0.01 M PBS containing a polyclonal antibody against bcl-2 (N-19, Santa Cruz Biotechnology,...

Somlyo Moving away from the intestine, I seem to remember that many years ago Geoff Burnstock convinced a poor electron microscopist to serially section a smooth muscle cell in the vas deferens, which he thought would be about 25 jim long. It turned out to be several times longer than this, and they showed that in the vas deferens smooth muscle there were neural synapses. 

Vuillet J. Correlative light and electron microscopic method for the visualization of the same in vitro cell using radioautography and serial sectioning, in Correlative Microscopy in Biology Instrumentation and Methods (Hayat MA, ed.), Academic Press, Orlando, FL, 1987, pp. 166-172. 

Large amounts of IAA-peptide in the seeds present in the tissue section caused bleeding of the chemiluminescence signal leading to a loss of anatomical detail. This may be alleviated by decreasing the exposure time of the film to a blot. To decrease the amount of antigen transferred to the blot, rinse the freshly cut tissue section in distilled water for 3-5 s. Alternatively, blot serial sections on the nitrocellulose. 

Fig. 35.3 FRAs expression within medial amygdala and ICN averaged over sets of three serial sections from MeA to MeP - for hamster (top) and mouse (below). The same two stimuli were used, each conspecific for one species, where it activates MeA and MeP, and heterospecific for the other, where it activates MeA and ICN. Control values for hamster were taken from animals perfused 15 min after exposure to HVF, before significant FRAs expression begins.

If there is no crystalline repeat in the stmcture, the methods of reconstmcting their 3D stmctures are serial sections if the object is large (thicker than 100 nm in the thinnest dimension) or tomography if the object is smaller. These techniques are widely used in biology, and may have important applications also in materials science. 

The fetuses are then examined. Full details of fetal examination are provided in other chapters of this book some techniques are performed on fresh fetuses, while other techniques require prior fixation of the fetuses. For rodents, one half of the fetuses in each litter should be examined for soft tissue (visceral) changes the remainder should be examined for skeletal changes. Rabbit fetuses should be examined for both soft tissue and skeletal abnormalities the heads from half of the fetuses should be examined by serial sectioning, with skeletal examination conducted on the remainder of the fetus. 

The Redbook recommends the Wilson serial section technique (see Chapter 19) for soft tissue examination, but microdissection techniques are equally applicable (see Chapter 20). 

The remaining fetuses are preserved for fixed visceral examination. This may be accomplished by serial sectioning (see Chapter 19) or by microdissection (see Chapter 20). 

The heads of approximately half of the fetuses in each litter are removed and fixed for subsequent examination by serial sectioning. 

Afrer the caesarean examination, all live fetuses are given a soft tissue examination and sexed. Following this, the heads of approximately half of the fetuses in each litter are removed and frxed for subsequent examination by serial sectioning (see Chapter 19). The eviscerated fetal carcasses are individually identified by the dam number and fetus number written on a card tag tied around the waist. 

Fetal Soft Tissue Examination by Serial Sectioning... 

This chapter descrihes the method used for serial sectioning and soft tissue examination of the Bouin s fixed fetus (mainly whole rat and or mouse fetuses or rabbit fetal heads) for the assessment of developmental and structural abnormalities. Fetuses are examined externally, together with the internal structures of the head as well as the thoracic and abdominal organs. 

Key words Serial sectioning, Fetal soft tissue, Bouin s, Wilson technique. Examination, Abnormalities... 

This technique facilitates soft tissue examination by serial sectioning (Modified Wilson technique (3)), as it is performed on preserved whole rat and mouse fetuses/rabbit heads (see Note 1). During the assessment all fetuses are examined both externally and internally. The principal. Definition main/primary of the procedure is to be able to distinguish between what is normal and abnormal, both structurally and developmentally, according to the stage of gestation. 

Transverse serial sections are now cut (consistently up to 1 mm thickness—see Note 4) through the neck and thorax down to the apex of the heart, just above the diaphragm (approximately 15 sections) (see Note 5). 

Fetuses are examined externally at fresh necropsy and during serial sectioning. Any abnormalities reported may be confirmed/ supported at serial sectioning evaluation (see Note 7). 

Following a gross external examination, all fetuses of non-rodent species, including the rabbit and minipig, are submitted to a fresh soft tissue examination by microdissection, after removal of the heads from half of the litter for serial sectioning. 

The microdissection technique is often considered simpler, quicker and easier to learn than complete serial sectioning. This is particularly true for non-rodent species when fresh examinations are performed at the time of caesarean section, so the results are available immediately. 

The use of magnetic resonance imaging of the fetal rat and rabbit head, as an alternative to the traditional methods of fixation and preparation of serial sections, is described. Labeled magnetic resonance images of normal head anatomy have been provided as a reference for use when evaluating the internal structures of... 

Smith BJ, Plowchalk DR, Sipes IG, Mattison DR (1991) Comparison of random and serial sections in assessment of ovarian toxicity. Reprod Toxicol, 5 379-383. 

Chytrid hydrogenosomes took rather different from the pictures of mitochondria in textbooks (Fig. 4). However, serial sectioning followed by electron microscopical analysis revealed a structure resembling the ultrastructure... 

Fig. 4 Serial sectioning of the hydrogenosomes of Neocallimastix sp. L2. A-D Bar = crometer h, hydrogenosomes r, ribosome globules (Munn et al. 1988). Asterisk, vesicular structures. From Voncken et al. 2002a, modified...

---