Fission yeasts

Booher, R., and Beach, D. (1986). Site-specific mutagenesis of cdc2+, a cell cycle control gene of the fission yeast Schizosaccaromyces pombe. Mol. Cell. Biol. 6 3523-3530. Booher, R. N., Alfa, C. E., Hyams, J. S., and Beach, D. H. (1989). The fission yeast cdc2/cdcl3/sucl protein kinase regulation of catalytic activity and nuclear localization. CeU 58 485-497. 

Bueno, A., and Russell, P. (1993). Two fission yeast B-type cyclins, cig2 and cdc 13, have different functions in mitosis. Mol. Cell. Biol. 13 2286-2297. 

Bueno, A Richardson, H., Reed, S. I., and Russell, P. (1991). A fission yeast B-type cyclin functioning early in the cell cycle. Cell 66 149-159. 

Ducommun, B., Draetta, P., Young, P and Beach, D. (1990). Fission yeast cdc25 is a cell-cycle regulated protein. Bichem. Biophys. Res. Commun. 167 301-307. 

Enoch, T and Nurse, P. (1990). Mutation of the fission yeast cell cycle control genes abolishes dependence of mitosis on DNA replication. Cell 60 665-673. 

Gautier, J., Norbury, C., Lohka, M., Nurse, P., and Mailer, J. (1988). Purified maturation-promoting factor contains the product of a Xenopus homologue of the fission yeast cell cycle control gene cdc2+. Cell 54 433-439. 

Gould, K. L., and Nurse, P. (1989). Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis. Nature 342 39-45. 

Jimenez, J., Alphey, L., Nurse, P, and Glover, D. M. (1990). Complementation of fission yeast cdc2,s and cdc25a mutants identifies two cell cycle genes from Drosophila a cdc2 homologue and string. EMBO J. 9 3565-3571. 

Kinoshita, N., Yamano, H., Niwa, H Yoshida, T., and Yanagida, M. (1993). Negative regulation of mitosis by the fission yeast protein phosphatase ppa2. Genes Dev. 7 1059-1071. 

Krek, W., Marks, J., Schmitz, N Nigg, E. A., and Simanis, V. (1992). Vertebrate p34c 2 phosphorylation site mutants effects upon cell cycle progression in the fission yeast Schizosaccammyces pombe. J. Cell Sci. 102 43-53. 

Moreno, S., Nurse, P, and Russell, P. (1990). Regulation of mitosis by cyclic accumulation of p80c 25 mitotic inducer in fission yeast. Nature 344 549-552. 

Nagata A., Igarashi, M Jinno, S., Suto, K and Okayama, H. (1991). An additional homolog of the fission yeast cdc25+ gene occurs in humans and is highly expressed in some cancer cells. New Biol. 3 959-968. 

Russell, R, and Nurse, P. (1986). cdc25+ functions as an inducer in the mitotic control of fission yeast. Cell 45 145-153. 

Simanis, V., and Nurse, R (1986). The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation. Cell 45 261-268. 

The accumulation of cyclin B and the absence of interphase after anaphase I fits well with data in fission yeast that indicate cyclin B marks the G2/M state of the... 

Nasmyth In the case of the weel system, not very much is known about how fission yeast is doing it. In budding yeast, it is the level of Cln3. 

The budding yeast securin has what appears to be a single stable partner, a 180 kDa protein called Espl (Ciosk et al 1998). In fission yeast, Cut2 had previously been found to be associated with Cut 1, an Espl homologue (Funabiki et al 1996b). 

Funabiki H, Hagan I, Uzawa S, Yanagida M 1993 Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast. J Cell Biol 121 961-976... 

Funabiki H, Yamano H, Kumada K, Nagao K, Hunt T, Yanagida M 1996a Cut2 proteolysis required for sister-chromatid seperation in fission yeast. Nature 381 438-441... 

Funabiki H, Kumada K, Yanagida M 1996b Fission yeast Cutl and Cut2 are essential for sister chromatid separation, concentrate along the metaphase spindle and form large complexes. EMBO J 15 6617-6628... 

Saka Y, Sutani T, Yamashita Y et al 1994 Fission yeast cut3 and cutl4, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis. EMBO J 13 4938-4952... 

Hunt There are differences between budding and fission yeast in the importance ofPdsl. 

Nurse Another difference is that the mitotic cohesin persists in fission yeast meiosis, which is not the case in budding yeast. I think what is exciting about this, however, are the similarities, so we shouldn t get too worried by the differences. We have talked a lot about cohesin, but I wondered whether it might be worth having some discussion of the S phase suppression. 

Nurse Actually, Cdcl8 is present in fission yeast meiosis, which is a Cdc6 equivalent. I think there is more of a story here. 

Nurse Work in starfish suggested that Cdk activity remained quite high, so that is the simplest model, and that is likely to be the case in fission yeast as well. We are cataloguing all the different components to see if we can work out the regulation of Cdk. It is consistent with but not yet proven that cdk regulation could regulate S phase between meiosis I and meiosis II. But I am worried about this mouse observation. Did you look at total HI histone kinase ... 

Nurse In fission yeast we can get asymmetrical divisions and make cells different sizes in various ways. Nuclear volume is influenced by subsequent cytoplasmic volume, and of course it is also directly influenced by ploidy. In an asymmetric division there will be a small nucleus and a big nucleus. 

Nurse Mal3, an Ebl homologue in fission yeast, interacts with a collection of other proteins that we have identified which are all localized at the ends of microtubules. They target the growth of microtubules to the cell ends in fission yeast in their absence, microtubules just keep growing. 

Fission yeast S. pombe is also referred to as safe - it was used for beer production in South Africa. Along with S. cerevisiae it is a very well established tool for studying basic biological processes like control of cell cycle and DNA repair processes [88], and its genome sequence is known [89]. 

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