Columns Hamilton

HPLC column - Hamilton PRP-1, 15 cm x 4.1 mm, 10 xm packing Flow Rate - 1.3 mL/min, Column Temperature - 35 C Mobile Phase A Water with 1.5 mM each hexane and heptane sulfonate, and 1.5 mM ammonium phosphate (as PO ), pH 6.70 Mobile Phase B 25% Acetonitrile with 1.5 mM each hexane and heptane sulfonate and 6.25 mM ammonium phosphate, pH 7.00 Gradient ... 

Separation on anion-exchange column (Hamilton PRP-XlOO) with 20 mmol L NH4H2PO4 (pH 5.6) mobile phase and a column temperature of 40 °C [separation time of seven As species 16 min As(V) and the ribose with the glycerol aglycone eluted in the dead volume] on-line ICP-MS detection... 

Enzymatic hydrolysis (trypsine) carried out for fauna samples sediments were treated with 1 mol L H3PO4 in an open focused MW system separation on anion-exchange column (Hamilton PRP-XlOO) step gradient elution with a phosphate-based mobile phase, pH 6-7.5 on-line ICP-MS... 

Water-methanol (1 1) extraction As(III) and AB separated on cation-exchange column (Hamilton PRP-X200) in pyridine formate 4mmolL pH 2.8 As(V),... 

MMA and DMA separated on anion-exchange column (Hamilton PRPX-100) with phosphate mobile phase lOmmol L. pH 6 on-line ICP-MS... 

AE column (Hamilton, PRP-XI00) with different phosphate-oxalate- and acetate-based mobile phases containing 3-20% of methanol ICP-MS detection and ESI-MS-MS for species identification and/or confirmation... 

Hamilton Company, Polymeric HPLC columns. Hamilton Co., Reno, NV, U.S.A. 

Method (II) which utilizes a PRP-1 column (Hamilton Co.) has also been used for the evaluation of lisinopril bulk drug. This method is, however, more cumbersome to use than method (I) which was found to offer better resolution for compounds of interest. 

DMA is dimethylarsenic acid. Column Hamilton PRP-1. Mobile phase 0.05 M CTAB-10% 1-propanol at pH 10.2. Reprinted from Ref. 16. 

C02 in a refractive index detector after digestion with persulfate by UV radiation. Acetate was measured by a High Pressure Liquid Chromatography (HPLC) using a reverse phase column (Hamilton PRP-X3(X)). 

The simplest unit employing vacuum fractionation is that designed by Canadian Badger for Dominion Tar and Chemical Company (now Rttgers VFT Inc.) at Hamilton, Ontario (13). In this plant, the tar is dehydrated in the usual manner by heat exchange and injection into a dehydrator. The dry tar is then heated under pressure in an oil-fired hehcal-tube heater and injected directly into the vacuum fractionating column from which a benzole fraction, overhead fraction, various oil fractions as side streams, and a pitch base product are taken. Some alterations were made to the plant in 1991, which allows some pitch properties to be controlled because pitch is the only product the distillate oils are used as fuel. 

Ethyl chloroformate [541-41-3] M 108.5, m -81 , b 94-95 , d 1.135, n 1.3974. Washed several times with water, redistd using an efficient fractionating column at atmospheric pressure and a CaCl2 guard tube to keep free from moisture [Hamilton and Sly J Am Chem Soc 47 435 1 925 Saunders, Slocombe and Hardy, J Am Chem SocT3 3796 1951]. LACHRYMATORY AND TOXIC. 

The luciferin produces a blue oxidation product during its purification process. In the DEAE chromatography of luciferin, this blue compound is eluted before the fractions of luciferin. The fractions of the blue compound were combined and purified by HPLC on a column of Hamilton PRP-1 (7 x 300 mm) using methanol-water (8 2) containing 0.1% ammonium acetate. The purified blue compound showed absorption peaks at 234, 254, 315, 370, 410, 590 (shoulder) and 633 nm. High-resolution FAB mass spectrometry of this compound indicated a molecular formula of C l C Nai m/z 609.2672 (M - Na + 2H)+, and mlz 631.2524 (M + H)+]. These data, together with the HNMR spectral data, indicated the structure of the blue compound to be 8. 

HPLC on a PRP-1 column (0.7 x 30.5 cm, Hamilton), with 65% acetonitrile containing 0.05% acetic acid, at a flow rate of 4 ml/min. To reverse the possible hydration of the molecules, the material obtained in step 3 is first evaporated to dryness, and redissolved in chloroform. Immediately before injection onto the HPLC column, an... 

Purification of the activation products (PMs). The methylamine activation product dissolved in methanol is purified by chromatography, first on a column of silica gel using a mixed solvent of chloroform/ethanol, followed by reversed-phase HPLC on a column of divinylbenzene resin (such as Jordi Reversed-Phase and Hamilton PRP-1) using various solvent systems suitable for the target substance (for example, acetonitrile/water containing 0.15% acetic acid). 

The Linear Algebraic Problem.—Familiarity with the basic theory of finite vectors and matrices—the notions of rank and linear dependence, the Cayley-Hamilton theorem, the Jordan normal form, orthogonality, and related principles—will be presupposed. In this section and the next, matrices will generally be represented by capital letters, column vectors by lower case English letters, scalars, except for indices and dimensions, by lower case Greek letters. The vectors a,b,x,y,..., will have elements au f it gt, r) . .. the matrices A, B,...,... 

A PRP -1 (Hamilton Reno, NV) reversed phase column was coated with cetylpyridinium and eluted with tetramethylammonium salicylate acetoni-trile water.89 The separation was comparable to that observed on conventional ion exchange. Coated phases were also used to separate oxalate complexes of manganese, cobalt, copper, and zinc.90 Reversed phase silica supports were coated with poly(N-ethyl-4-vinylpyridinium bromide), poly(dimethydiallylammonium chloride), poly(hexamethyleneguanidinium... 

Hamilton, P. B., Ion exchange chromatography of amino acids. A single column, high resolving, fully automatic procedure, Anal. Chem., 35, 2055, 1963. 

Lake Erie, Hamilton Harbour 1984 Water column vs. sediments ... 

The prepared mixtures were placed in the extraction vessel, and stirred for 2 h and then left to settle for 4 h. Samples were taken by a syringe (Gaschromatographic s Hamilton 0.4 p,L) from both the upper (methylcyclohexane) phase and lower layers (aromatic phase). Both phases were analyzed using Konik gas chromatography (GC) equipped with a thermal conductivity detector (TCD) and Shimadzu C-R2AX integrator. A 2 m x 2 mm column was used to separate the components... 

A good general-purpose anion-exchange column for non-suppressed applications is the Hamilton PRP-XlOO column. As the selection of eluents used with this column is quite varied, the reader is referred to Hamilton column literature for specific eluent recommendations. The Hamilton PRP-XlOO column is most likely prepared via chemical modification of a porous polymeric substrate. 

Fig. 8.5 Capsaicin accumulates in blisters/vesicles on surface of placenta, left panel) Total ion chromatogram of oil in habanero vesicle. The oil in the vesicle was collected directly with a Hamilton syringe, diluted with hexane, and analyzed on a varian GC-MS, DB-5 column. The capsaicin peak was identified based on match with NIST MS library, right upper panel) A stereoscope view of habanero placenta (5x magnification) and seeds are visible upper left panel). Arrows indicate blisters, right lower panel) A scanning electron micrograph of habanero placenta (40 X magnification). A color version of the image is on line...

An improved high pressure liquid chromatographic (HPLC) procedure Tor the PSP toxins is described. The method involves separation of the toxins on a polystyrene divinylbenzene resin colunn (Hamilton, PRP-1) in the reversed phase mode using heptane and hexane sulfonic acids as ion-pairing reagents. Detection of the toxins is by fluorescence following post-column alkaline periodate oxidation. The sensitivity of the HPLC method is better than the standard mouse bioassay by at least a factor of four for each of the individual toxins. 

Toxin Separations. A number of columns have been evaluated for suitability using the HPLC method. Of all columns tested to date, the Hamilton PRP-1 column (polystyrene divinylbenzene resin) has proven to be the most useful. Toxin retention on this column is controlled by 1) methanol concentration, 2) mobile phase ionic strength, 3) chain length of the ion-pair reagent, and M) mobile phase pH. 

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