Collagen scaffolds

Tedder ME et al (2009) Stabilized collagen scaffolds for heart valve tissue engineering. Tissue Eng Part A 15(6) 1257-1268... 

Fig. 7 MSC differentiated neuronal cells on electrospun PLCL/collagen scaffolds (a) cell morphology by SEM, (b) expression of neurofilament protein, and (c) expression of nestin. Reproduced (Fig7b, 7c) with permission from Prabhakaran et al. [162]...

Zhong S et al (2006) An aligned nanofibrous collagen scaffold by electrospinning and its effects on in vitro fibroblast culture. J Biomed Mater Res A 79(3) 456-463... 

Initially, natural decellularized arterial (ECM) was previously used as a scaffold however, this material was found to form matrices too tight for cellular migration when seeded with cells (36). To create more porous scaffolds, pure elastin and pure collagen scaffolds derived from arterial ECM were generated. The elastin scaffolds exhibited 120 pm infiltration of fibroblast cells in vitro and in vivo models showed improved cell infiltration and repopulation of the scaffold... 

PDLLA)/chitosan scaffolds measured using MTT assay seeded onto the PDLLA/chitosan scaffolds scaffolds increased on increasing the weight ratio of the chitosan component, were able to preserve the phenotype of chondrocytes, and also supported the production of type II collagen scaffolds are able to promote the attachment and proliferation of chondrocytes [104]... 

Glowacki J, Mizuno S (2008) Collagen scaffolds for tissue engineering. Biopolymers 89 338-344... 

Peng L, Cheng XR, Wang JW et al (2006) Preparation and evaluation of porous chitosan/ collagen scaffolds for periodontal tissue engineering. J Bioactive Compatible Polym 21 207-220... 

Fig. 7 Molecular and structural schematics of hybrid polymer network scaffold comprised of chitosan-embedded collagen scaffold crosslinked by PEG-DBA and EDC/NHS [96]...

Kemp, P.D., Cavallaro, J.F., and Hastings, D.N. 1995. Effects of carbodiimide crosslinking and load environment on the remodeling of collagen scaffolds. Tissue Eng. 1 71-79. 

The cell-binding properties of chitosan have led to numerous studies where chitosan is used as a surface coating. Chitosan was employed as a surface modification of poly(e-caprolactone) scaffolds and in vitro studies with fibroblasts showed significantly improved cell attachment and proliferation when chitosan was present. In another study, polyurethane scaffolds were prepared and surface-modified with chitosan for the same reason. This study showed that on the modified scaffold a monolayer of endothelial intima was formed. The incorporation of collagen with chitosan as a chi-tosan-collagen scaffold enhanced the resultant scaffold s ability to support cell attachment and is a strategy similar to the incorporation of peptide sequences. ... 

Q.A. Lv, Q.L. Feng, K. Hu, F.Z. Cui, Three-dimensional fibroin/ collagen scaffolds derived from aqueous solution and the use for HepG2 culture. Polymer 46 (2005) 12662-12669. 

C. Shi, Q. li, Y. Zhao, W. Chen, B. Chen, Z. Xiao, et al.. Stem-cell-capturing collagen scaffold promotes cardiac tissue re- [197] generation, Biomaterials 32 (2011) 2508-2515. 

X. Li, Z. Xiao, J. Han, L. Chen, H. Xiao, F. Ma, et al.. Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair, Biomaterials 34 (2013) 5107-5116. 

C. Qu, Y. Xiong, A. Mahmood, D.L. Kaplan, A. Goussev, R. Ning, et al., Treatment of traumatic brain injury in mice with bone marrow stromal cell-imtregnated collagen scaffolds, J. Neurosurg. Ill (2009) 658. 

P. Soltysiak, M.E. Hollwarth, A.K. Saxena, Comparison of suturing techniques in the formation of collagen scaffold tubes for composite tubular organ tissue engineering, Biomed. Mater. Eng. 20 (2010) 1-11. 

Y. Liu, S. Bharadwaj, S.J. Lee, A. Atala, Y. Zhang, Optimization of a natural collagen scaffold to aid cell—matrix penetration for uro-logic tissue engineering, Biomaterials 30 (2009) 3865-3873. 

FIGURE 4.11 Cellular viability of osteoblasts on (a) cellulose acetate scaffolds and (b) cellulose acetate collagen scaffolds. The green cells are viable and the red ones are nonviable (live/Dead staining) [170]. 

S. Neuss, et al.. Long-term survival and bipotent terminal differentiation of human mesenchymal stem cells (hMSC) in combination with a commercially available three-dimensional collagen scaffold. Cell Transplant. 17 (2008) 977-986. 

D. von Heimburg, et al.. Preadipocyte-loaded collagen scaffolds with enlarged pore size for improved soft tissue engineering, Int. J. Artif. Organs 26 (2003) 1064-1076. 

Y. Hiraoka, et al.. In situ regeneration of adipose tissue in rat fat pad by combining a collagen scaffold with gelatin microspheres containing basic fibroblast growth factor. Tissue Eng. 12 (2006) 1475-1487. 

K.M. Brouwer, W.F. Daamen, N. van Lochem, D. Reijnen, R.M.H. Wijnen, T.H. van Kuppevelt, Construction and in vivo evaluation of a dual layered collagenous scaffold with a radial pore structure for repair of the diaphragm, Acta Biomater. 9 (6) (2013) 6844—6851. 

E. Song, S. Yeon Kim, T. Chun, H.J. Byun, Y.M. Lee, Collagen scaffolds derived from a marine source and their biocompatibility. Biomaterials 27 (15) (2006) 2951-2961. 

FIGURE 21.1 Photographs of some commercially available collagen scaffolds. 

Kose, G., Korkusuz, F., Korkusuz, P., Hasirci, V. In vivo tissue engineering of bone using poly(3-hydroxybutyric acid-co-3- hydroxyvaleric acid) and collagen scaffolds. Tissue Eng. 10, 1234-1250 (2004)... 

Shi, D., Cai, D. Zhou, C. (2005a) [Fabrication and properties of a composite chitosan/ type II collagen scaffold for tissue engineering cartilage]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi, 19, 278-82. 

---