Capillary electrophoresis analysis

Hurst, W.J. and Martin, R.A. Jr, The quantitative determination of caffeine in beverages using capillary electrophoresis analysis, 21,389-91,1993. 

FIGURE 15.4 Computer record of a two-dimensional capillary electrophoresis analysis of a protein homogenate prepared from ahiopsy obtained from the fundus of a Barrett s esophagus patient. The data were generated hy performing 1 s transfers between capillaries and a 9 s second-dimension separation. The first-dimension separation employed the same buffer as the CSE separation in Fig. 15.1 and the second-dimension separation employed the same buffer as the MECC separation in Fig. 15.1. 

FIGURE 15.10 Landscape image of a two-dimensional capillary electrophoresis analysis of the protein content of a single fixed MCF-7 breast-cancer cell. The data consist of a few high-amplitude components. 

Michels, D.A., Hu, S., Damhrowitz, K.A., Eggertson, M.J., Lauterhach, K., Dovichi, NJ. (2004). Capillary sieving electrophoresis-micellar electrokinetic chromatography fully automated two-dimensional capillary electrophoresis analysis of Deinococcus radiodurans protein homogenate. Electrophoresis 25, 3098-3105. 

Kaale, E., Leonard, S., Van Schepdael, A., Roets, E., and Hoogmartens, J. (2000). Capillary electrophoresis analysis of gentamicin sulphate with UV detection after pre-capillary derivatization with 1,2-phthalic dicarboxaldehyde and mercaptoacetic acid. /. Chromatogr. A 895, 67—79. 

Lurie, I. S., Hays, P. A., and Parker, K. (2004). Capillary electrophoresis analysis of a wide variety of seized drugs using the same capillary with dynamic coatings. Electrophoresis 25, 1580—1591. 

RC Tim, RA Kautz, BL Karger. Ultratrace analysis of drugs in biological fluids using affinity probe capillary electrophoresis analysis of dorzolamide with fluorescently labeled carbonic anhydrase. Electrophoresis 21 220—226, 2000. 

K Shimura, BL Karger. Affinity probe capillary electrophoresis Analysis of recombinant human growth hormone with a fluorescent-labeled antibody fragment. Anal Chem 66 9-15, 1994. 

R. Gillette, and J. V. Sweedler, Capillary Electrophoresis Analysis of NO Synthase Related Metabolites in Single Neurons, Anal. Chem. 1998, 70, 2243. 

Schmitt-Kopplin, P., Garmash, A. V., Kudryavtsev, A. V., Menzinger, F., Perminova, I. V., Hertkorn, N., Freitag, D., Petrosyan, V. S., and Kettrup, A. (2001a). Quantitative and qualitative precision improvements by effective mobility-.scale data transformation in capillary electrophoresis analysis. Electrophoresis 22,77-87. 

Methods to determine the potential biological activity of products obtained through recombinant DNA techniques are of fundamental importance. Despite the existence of numerous physicochemical techniques to characterize the protein product structure and the presence of contaminants, they provide little, if any, information about its biological potency. A bioassay is defined as a functional test, and no physicochemical test can measure the function. However, for some peptide hormones, which are less complex in structure than most cytokines, well defined physicochemical tests may be used to estimate biological activity for instance, the capillary electrophoresis analysis of a protein s isoform content if the specific activity of each one is known. 

Mercier, J.-P., Morin, Ph., Dreux, M. and Tam-bute, A. Capillary electrophoresis analysis of chemical warfare agent breakdown products, J. Chromatogr., A, 741, 279 (1996). 

Lagally ETS, P. C., Mathies, R. A. Monolithic integrated microfluidic DNA amplification n and capillary electrophoresis analysis system. Sens Actuators B 2000 B63 138-146. 

G. Teshima and S.-L. Wu, Capillary electrophoresis analysis of recombinant proteins. Methods Enzymol. 271 264 (1996). 

Clark R, Katzmann JA, Kyle R, Fleisher M, Landers JR Differential diagnosis of gammopathies by automated capillary electrophoresis Analysis of serum samples problematic by agarose gel electrophoresis. Electrophoresis 1998 19 2479-84. 

APPLICATION OF CHEMOMETRICS IN CAPILLARY ELECTROPHORESIS ANALYSIS OF HERBAL MEDICINES... 

TM Phillips, JJ Chmielinska. Immunoaffinity capillary electrophoresis analysis of cyclosporin in tears. Biomed Chromatogr 8 242-246, 1994. 

Figure 14. Microfluidic realization of capillary electrophoresis analysis on the electrokinetic platform. (Adapted from [123], ( Agilent Technologies, Inc. 2007. Reproduced with permission, courtesy of Agilent Technologies, Inc.) After the sample has been transported to the junction area (a) it is metered by the activated horizontal flow and injected into the separation channel (b). Therein, the sample components are electrophoreticaUy separated (c) and readout by their fluorescence signal (d). The complete microfluidic CE-chip is depicted in the center.

When xylobiose was tested as a substrate, deviation from Michaelis-Menten kinetics consistent with transglycosylation activity was observed at higher substrate concentrations (up to 16 mM xylobiose was tested). Capillary electrophoresis analysis of the reaction products from xylobiose hydrolysis showed production of significant xylose and xylotriose, corroborating the transglycosylation activity inferred by the kinetic data. Thus, under the... 

Landers, J. R, Schuchard, M D., Subramaniam, S., SismeUch, T. and Spelsberg, T. C. High-performance capillary electrophoresis analysis of chloramphenicol acetyl transferase activity, J. Chrorrmtogr., 603, 247-257, 1992a. 

Kraly JR, Jones MR, Gomez DG Dickerson JA, Harwood MM, Eggertson M, Paulson TG et al. Reproducible two-dimensional capillary electrophoresis analysis of Barrett s esophagus tissues. Anal Chem 2006 78 5977-86. 

Practical Applications of Capillary Electrophoresis Analysis of DNA Molecules. 230... 

PRACTICAL APPLICATIONS OF CAPILLARY ELECTROPHORESIS ANALYSIS OF DNA MOLECULES... 

Joucla, G, Brando, T., Remaud-Simeon, M., Monsan, P. and Puzo, G. Capillary electrophoresis analysis of glucooUgosaccharide regioisomers. Electrophoresis, 25, 861, 2004. 

Cruz, L. et al.. Nitrite and nitrate levels in individual molluscan neurons Single-cell capillary electrophoresis analysis, J. Neurochem., 69, 110, 1997. 

Floyd, P.D. et al.. Capillary electrophoresis analysis of nitric oxide synthase related metabolites in single identified neurons. Anal. Chem., 70, 2243, 1998. 

---