Capillary electrophoresis qualitative analysis

Capillary electrophoresis is an electrophoresis technique in which the mixture components are separated in a capillary tube and detected with an on-line detector after the separation occurs. The advantages include smaller quantity of sample and qualitative and quantitative analysis in a much shorter time. 

Soga, T., Kakazu, Y., Robert, M., Tomita, M., andNishioka, T. (2004). Qualitative and quantitative analysis of amino acids by capillary electrophoresis-electrospray ionization-tandem mass spectrometry. Electrophoresis 25, 1964—1972. 

Voirin, B. et al.. Separation of flavone C-glycosides and qualitative analysis of Passiflora incarnata L. by capillary zone electrophoresis, Phytochem. Anal, 11, 90, 2000. 

Schmitt-Kopplin, P., Garmash, A. V., Kudryavtsev, A. V., Menzinger, F., Perminova, I. V., Hertkorn, N., Freitag, D., Petrosyan, V. S., and Kettrup, A. (2001a). Quantitative and qualitative precision improvements by effective mobility-.scale data transformation in capillary electrophoresis analysis. Electrophoresis 22,77-87. 

The quality of a calibration also depends on the quality of the reference samples used. Glucuronic acid is a particular example the enzymatic method for this analysis is not reliable in that it lacks robustness. This problem has not been identified in the literature and is probably linked to non-identified matrix effects. The first FTIR calibrations were carried out using the enzymatic method as a reference and, even though gluconic acid has highly qualitative absorption characteristics, the results obtained by FTIR were unreliable. Subsequent calibrations carried out using more reliable methods such as capillary electrophoresis as a reference technique have resulted in FTIR analyses of high quality in terms of precision and accuracy. 

Other techniques, such as capillary electrophoresis and [matric-assisted laser desorption ionization] mass spectrometry, provide qualitative analyses, often with greater speed and sensitivity. Nevertheless, AAA by high-performance liquid chromatography (HPLC) complements other structural analysis techniques, such as peptide sequencing, and remains indispensable for quantifying proteinaceous materials. 

Capillary electrophoresis (CE) is a valuable tool for qualitative and quantitative analysis of a wide variety of biomolecules [10,11]. Separation by CE combines features from classical methods of polyacrylamide gel electrophoresis (PAGE) and HPLC. Although not as frequently employed, CE can... 

Aside from conventional electrophoretic methods, capillary electrophoresis (CE) is considered as a novel electrophoretic technique for protein separation with advantages of being easy, rapid, automatic, using trace amount of test samples, and qualitative as well as quantitative within one analysis... 

Capillary electrophoresis technology has become an indispensable tool for forensic scientists in the biology field since it is able to provide valuable information to aid in the process of law enforcement. The primary application of the technique is in the qualitative analysis of STRs. Isolation of STR mixtures is also possible using relative peak heights. Other applications of CE include quantitative analysis of PCR products, mtDNA sequencing, and mutation detection for the analysis of plant and bacterial DNA. Based on the performance of the methods illustrated above, it is reasonable to expect future researchers and practitioners to continue working to exploit the capabilities of this robust scientific technique and its application to criminal investigations. 

Gerritsma, J., Sinnige, D., Drieze, C., Sittrop, B., Houtsma, P, Hulshorst-Jansen, N., and Huisman, W. Quantitative and qualitative analysis of haemoglobin variants using capillary zone electrophoresis. 

Peng, Y., Liu, F., and Ye, J., Quantitative and qualitative analysis of flavonoids markers in Frucus aurantii of different geographical origin by capillary electrophoresis with electrochemical detection, J. Chromatogr. B, 830, 224, 2006. 

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