Capillary electrophoresis, peptide analysis

Capillary Liquid Chromatography Coupled with Capillary Electrophoresis for Analysis of Unlabeled Peptides and Proteins... 

A. J. Tomlinson and S. Naylor, Systematic development of on-line membrane preconcentration-capillary electrophoresis-mass spectrometry foi analysis of peptide mixtures, J. Capillary Electrophoresis 225-233 (1995). 

Figure 11.18 Schematic diagram of an in-line SPE unit for CE using (a) polyester wool frits to hold the sorbent, or (b) a paiticle-loaded membrane. Reprinted from Journal of Capillary Electrophoresis, 2, A. J. Tomlinson and S. Naylor, Enhanced performance membrane preconcenti ation-capillary electrophoresis-mass spectiometi y (mPC-CE-MS) in conjunction with ti ansient isotachophoresis for analysis of peptide mixtures, pp 225-233, 1995, with permission from ISC Teclmical Publications Inc.

A. W. Moore-Jr, J. P. Lamiann-Jr, A. V. Lemmo and J. W. Jorgenson, Two-dimensional liquid chromatography-capillary electrophoresis teclmiques for analysis of proteins and peptides . Methods Enzymol. 270 401-419 (1996). 

Advis, J. P., Hernandez, L., and Guzman, N. A., Analysis of brain neuropeptides by capillary electrophoresis determination of luteinizing hormone-re-leasing hormone from ovine hypothalamus, Peptide Res., 2, 389, 1989. 

Janini, G.M., Chan, K.C., Conrads, T.P., Issaq, H.J., Veenstra, T.D. (2004). Two-dimensional liquid chromatography-capillary zone electrophoresis—sheathless electrospray ionization-mass spectrometry evaluation for peptide analysis and protein identification. Electrophoresis 25, 1973-1980. 

Larsson M, Lutz ES. 2000. Transient isotachophoresis for sensitivity enhancement in capillary electroporesis-mass spectrometry for peptide analysis. Electrophoresis 21 2859. 

Catai, J. R., Somson, G. W., and de Jong, G. J. (2004). Efficient and reproducible analysis of peptides by capillary electrophoresis using noncovalently bilayer-coated capillaries. Electrophoresis 25, 817-824. 

Stutz, H. (2005). Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix-assisted laser desorption/ionization and electrospray—mass spectrometry detection. Electrophoresis 26, 1254—1290. 

Johannesson, N., Wetterhall, M., Markides, K. E., and Bergquist, J. (2004). Monomer surface modifications for rapid peptide analysis by capillary electrophoresis and capillary electrochromatography coupled to electrospray ionization-mass spectrometry. Electrophoresis 25, 809-816. 

Yang, Q., Benson, L. M., Johnson, K. L., and Naylor, S. (1999). Analysis of lipophilic peptides and therapeutic drugs-on-line- nonaqueous capillary-electrophoresis mass-spectrometry. /. Biochem. Biophys. Methods 38, 103 — 121. 

Zhang, B., Foret, F, and Karger, B. L. (2000). A microdevice with integrated liquid junction for facile peptide and protein analysis by capillary electrophoresis/electrospray mass spectrometry. Anal. Chem. 72, 1015-1022. 

L Fang, R Zhang, ER Williams, RN Zare. On-line time-of-flight mass spectro-metric analysis of peptides separated by capillary electrophoresis. Anal Chem 66 3696-3701, 1994. 

Finally, when RPC methods are used in preparative studies with peptides, the opportunity routinely exists for subsequent analysis of the recovered fractions by a variety of analytical methods including high-speed RP-HPLC, HP-IEX, HP-HILIC, or HP-IMAC, zonal or micellar electrokinetic high-performance capillary electrophoresis (HP-CZE and MECK-CZE), capillary electrochromatography (CEC), or capillary isotachophoresis. The combination of the RPC information, drawn from the In k versus i > plots, with the data derived from on-line spectroscopic detection thus readily provides a comprehensive opportunity to assess the purity of an isolated peptide, many of the physicochemical features of the interaction, as well as a means to optimize the resolution in the RPC separation. 

KM De Antonis, PR Brown. Analysis of derivatized peptides using high performance liquid chromatography and capillary electrophoresis. Adv Chromatogr 37 425-452, 1997. 

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