Subcellular Analysis by Capillary Electrophoresis

4 Direct Sampling of Dopamine from Mammalian Cell Cytoplasm. 594 

5 Detection of Individual Mitochondria Sampled from Muscle Tissue Cross 

1 Isolation of Mitochondria from Mammalian Cell Culture. 597 

Many cellular processes are localized within specific subcellular compartments therefore, recent bioanalytical research has been aimed at utilizing the natural organization found within cells to reduce the complexity of biological samples. Subcellular compartments include (i) distinct, vesicular organelles, such as nuclei, mitochondria, and acidic organelles (ii) indistinct, continuous organelles 

Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques 

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In spite of the reduction in complexity that can be achieved by cellular fractionation, an analytical separation is frequently required to separate one or more components from the cellular milieu. As evidenced throughout this book, capillary electrophoresis (CE) provides high resolution and separation efficiency, both of which are necessary for subcellular analysis. In addition, CE is advantageous because it requires very little sample volume, typically less than a nanoliter, and generally very little sample preparation. Hence, capillaries have been used to directly sample subcellular compartments within neurons, oocytes, and muscle tissue sections. They have also been used to analyze individual organelles from a single cell following on-column lysis." ... 

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