Capillary electrophoresis genetic analyses

The microfluidic chip uniquely facilitates high-throughput genetic analysis. For instance, 12 different samples were analyzed in parallel by capillary array electrophoresis (CAE) see Figure 9.20. The two-color method was used for... 

Medintz, I.L., Paegel, B.M., Blazej, R.G., Emrich, C.A., Berti, L., Scherer, J.R., Mathies, R.A., High-performance genetic analysis using microfabricated capillary array electrophoresis microplates. Electrophoresis 2001, 22, 3845-3856. 

One cannot overestimate the importance of fluorescence detection in high-performance capillary electrophoresis (HPCE) [1], The success of the human genome project along with the forthcoming revolutions in forensic testing and genetic analysis might not have occurred without the sensitivity and selectivity of laser-induced fluorescence (LIF) detection. 

Emrich CA, Tian H, Medintz IL, Mathies RA. Micro-fabricated 384-lane capillary array electrophoresis bioanalyzer for ultrahigh-throughput genetic analysis. Anal Chem 2002 74 5076-83. 

Simpson, P. C., Roach, D., Woolley, A. T., Thorsen, T., Johnston, R., Sensabaugh, G. E, and Mathies, R. A. High-throughput genetic analysis using microfabricated 96-sample capillary array electrophoresis microplates. Proc. Natl. Acad. Sci. U.S.A. 95 2256-2261, 1998. 

In this chapter we summarize the complex issues that are involved in the analysis of sizing DNA by capillary electrophoresis (CE), and how chemomet-ric methods can help to optimize a high number of interrelated variables. It is impressive to observe how diverse is the obtainable biological information despite the size of the double-stranded DNA molecule. We also briefly introduce some typical genetic assays that rely on sizing DNA molecules, and how some chemometric approaches are used to correlate sizes of DNA with population and or evolution of species. 

Giovannoli, C., Anfossi, L., Tozzi C., Giraudi, G, and Vanni, A. DNA separation by capillary electrophoresis with hydrophilic substituted celluloses as coating and sieving polymers. Apphcation to the analysis of genetically modified meals, J. Sep. ScL, 27, 1551, 2004. 

Simpson, P.C. et al.. High-throughput genetic analysis using microfabricated 96 sample capillary array electrophoresis microplates. Proc. Natl. Acad. Sci. USA, 95, 2256, 1998. 

Medintz IL, Paegel BM, Blazej RG, et al. (2001) High-performance genetic analysis using microfabricated capillary array electrophoresis microplates. Electrophoresis 22 3845-3856. 

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