Affinity probe capillary electrophoresis

RC Tim, RA Kautz, BL Karger. Ultratrace analysis of drugs in biological fluids using affinity probe capillary electrophoresis analysis of dorzolamide with fluorescently labeled carbonic anhydrase. Electrophoresis 21 220—226, 2000. 

K Shimura, BL Karger. Affinity probe capillary electrophoresis Analysis of recombinant human growth hormone with a fluorescent-labeled antibody fragment. Anal Chem 66 9-15, 1994. 

K Shimura, K Kasai. Determination of the affinity constants of concanavalin A for monosaccharides by fluorescence affinity probe capillary electrophoresis. Anal Biochem 227 186-194, 1995. 

Haes, A.J., Giordano, B.C. and Collins, G.E. (2006) Aptamer-based detection and quantitative analysis of ricin using affinity probe capillary electrophoresis. Anal Chem, 78, 3758-3764. 

German, I. D., Buchanan, D., Kennedy, R. T. (1998). Aptamers as ligands in affinity probe capillary electrophoresis. Anal Chem 70, 4540-4545. 

Nonequilibrium capillary electrophoresis of the equilibrium mixtures (NECEEM) is a unique KCE method that facilitates finding both and off from a single electropherogram. Mechanistically, it is similar to previously introduced affinity probe capillary electrophoresis (APCE) ° and falls... 

Affinity probe capillary electrophoresis (APCE) uses a fluorescently labeled ligand to bind a receptor. Separation of the bound and free affinity probe has been achieved with CE with LIE detection. One advantage of the rapid CE method is that if the separation is rapid compared to the dissociation time, affinity constants can be determined. Competition experiments can be performed to observe the conditions of complex formation. For example, a fluorescent GTP analog was used as an affinity probe and G protein receptor subunits as the ligand. The complex and free fluorescent probe were separated within 20 s and Ras-like G protein complexes could be separated in less than 15 s. The rapid separations allowed kinetics of binding to be studied. 

Jameson, E. E. et al. Detection of G proteins by affinity probe capillary electrophoresis using a fluorescently labeled GTP analogue. Anal. Chem. 75, 4297, 2003. 

Hafner, F T. et al. Noncompetitive immunoassay of small analytes at the femtomolar level by affinity probe capillary electrophoresis Direct analysis of digoxin using a uniform-labeled scFv immunoreagent. Anal. Chem. 72, 5779, 2000. 

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