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Concentration substrate

The rate of each of the steps in the overall electrode process has a simple dependence on the concentration of the electroactive species in the bulk of the solution, as in the following examples. [Pg.198]

It has been seen from the above simple examples that the concentration of the substrate has a profound effect on the rate of the electrode process. It must be remembered, however, that the process may show different reaction orders in the different potential regions of the curve. Thus, electron transfer is commonly the slow step in the Tafel region and diffusion control in the plateau region and these processes may have different reaction orders. Even at one potential the reaction order may vary with the substrate concentration as, for example, in the case discussed above where the electrode reaction requires adsorption of the starting material. [Pg.199]

Moreover, the product of an electrode process may also vary with the substrate concentration. In particular, this occurs when the reactive intermediate can further react by either a first-order or a higher-order chemical process or when the intermediate is able to react with molecules of the starting material. Examples of the latter type of reaction are [Pg.199]

At concentrations above 10 a complex series of reactions, ending with the formation of 4,4-methylenebis (N,N-dimethylaniline), occur. The extra methylene group comes from a further molecule of starting material. Finally, at even higher concentrations, the product becomes the dye, crystal violet or its leuco-form. [Pg.200]

The substrate concentrations are interesting variables when a mixture of two electroactive species is oxidized or reduced. If we take the example of two species Hi and Rj which are oxidized at sufficiently different potentials that two clear waves are obtained on an i-E curve, an electrolysis carried out at a potential on the plateau of the first wave must occur via the route [Pg.200]


Michaelis constant An experimentally determined parameter inversely indicative of the affinity of an enzyme for its substrate. For a constant enzyme concentration, the Michaelis constant is that substrate concentration at which the rate of reaction is half its maximum rate. In general, the Michaelis constant is equivalent to the dissociation constant of the enzyme-substrate complex. [Pg.262]

A plot of equation 13.18, shown in figure 13.10, is instructive for defining conditions under which the rate of an enzymatic reaction can be used for the quantitative analysis of enzymes and substrates. Eor high substrate concentrations, where [S] Kjq, equation 13.18 simplifies to... [Pg.637]

Plasteins ate formed from soy protein hydrolysates with a variety of microbial proteases (149). Preferred conditions for hydrolysis and synthesis ate obtained with an enzyme-to-substrate ratio of 1 100, and a temperature of 37°C for 24—72 h. A substrate concentration of 30 wt %, 80% hydrolyzed, gives an 80% net yield of plastein from the synthesis reaction. However, these results ate based on a 1% protein solution used in the hydrolysis step this would be too low for an economical process (see Microbial transformations). [Pg.471]

Eor measurement of a substrate by a kinetic method, the substrate concentration should be rate-limiting and should not be much higher than the enzyme s K. On the other hand, when measuring enzyme activity, the enzyme concentration should be rate-limiting, and consequentiy high substrate concentrations are used (see Catalysis). [Pg.38]

The rate of aqueous ozonation reactions is affected by various factors such as the pH, temperature, and concentration of ozone, substrate, and radical scavengers. Kinetic measurements have been carried out in dilute aqueous solution on a large number of organic compounds from different classes (56,57). Some of the chemistry discussed in the foUowing sections occurs more readily at high ozone and high substrate concentrations. [Pg.493]

The relative contributions from these processes strongly depend on the reaction conditions, such as type of solvent, substrate and water concentration, and acidity of catalyst (78,79). It was also discovered that in acid—base inert solvents, such as methylene chloride, the basic assistance requited for the condensation process is provided by another silanol group. This phenomena, called intra—inter catalysis, controls the linear-to-cyclic products ratio, which is constant at a wide range of substrate concentrations. [Pg.46]

Fig. 1. Reaction velocity as a function of substrate concentration for a reaction obeying MichaeHs-Menten kinetics. Fig. 1. Reaction velocity as a function of substrate concentration for a reaction obeying MichaeHs-Menten kinetics.
Enzyme Assays. An enzyme assay determines the amount of enzyme present in sample. However, enzymes are usually not measured on a stoichiometric basis. Enzyme activity is usually determined from a rate assay and expressed in activity units. As mentioned above, a change in temperature, pH, and/or substrate concentration affects the reaction velocity. These parameters must therefore be carefully controlled in order to achieve reproducible results. [Pg.288]

P = the concentration of predators S = substrate concentration (food for prey)... [Pg.2148]

The Michaelis-Menten scheme nicely explains why a maximum rate, V"max, is always observed when the substrate concentration is much higher than the enzyme concentration (Figure 11.1). Vmax is obtained when the enzyme is saturated with substrate. There are then no free enzyme molecules available to turn over additional substrate. Hence, the rate is constant, Vmax, and is independent of further increase in the substrate concentration. [Pg.206]

The substrate concentration when the half maximal rate, (Vmax/2), is achieved is called the Km. For many simple reactions it can easily be shown that the Km is equal to the dissociation constant, Kd, of the ES complex. The Km, therefore, describes the affinity of the enzyme for the substrate. For more complex reactions, Km may be regarded as the overall dissociation constant of all enzyme-bound species. [Pg.206]

Figure 11.1 A plot of the reaction rate as a function of the substrate concentration for an enzyme catalyzed reaction. Vmax is the maximal velocity. The Michaelis constant. Km, is the substrate concentration at half Vmax- The rate v is related to the substrate concentration, [S], by the Michaelis-Menten equation ... Figure 11.1 A plot of the reaction rate as a function of the substrate concentration for an enzyme catalyzed reaction. Vmax is the maximal velocity. The Michaelis constant. Km, is the substrate concentration at half Vmax- The rate v is related to the substrate concentration, [S], by the Michaelis-Menten equation ...
Rates that are independent of aromatic substrate concentration have been found for reaction of benzyl chloride catalyzed by TiCl4 or SbFj in nitromethane. This can be interpreted as resulting from rate-determining formation of the electrophile, presumably a benzyl cation. The reaction of benzyl chloride and toluene shows a second-order dependence on titanium tetrachloride concentration under conditions where there is a large excess of hydrocarbon. ... [Pg.581]

Equation 1-106 predicts that the initial rate will be proportional to the initial enzyme concentration, if the initial substrate concentration is held constant. If the initial enzyme concentration is held constant, then the initial rate will be proportional to the substrate concentration at low substrate concentrations and independent of the substrate concentration at high substrate levels. The maximum reaction rate for a given total enzyme concentration is... [Pg.24]

Kinetic studies involving enzymes can principally be classified into steady and transient state kinetics. In tlie former, tlie enzyme concentration is much lower tlian that of tlie substrate in tlie latter much higher enzyme concentration is used to allow detection of reaction intennediates. In steady state kinetics, the high efficiency of enzymes as a catalyst implies that very low concentrations are adequate to enable reactions to proceed at measurable rates (i.e., reaction times of a few seconds or more). Typical enzyme concentrations are in the range of 10 M to 10 ], while substrate concentrations usually exceed lO M. Consequently, tlie concentrations of enzyme-substrate intermediates are low witli respect to tlie total substrate (reactant) concentrations, even when tlie enzyme is fully saturated. The reaction is considered to be in a steady state after a very short induction period, which greatly simplifies the rate laws. [Pg.833]

Figure 11-1a. Simple Michaelis-Menten kinetics. At low substrate concentration... Figure 11-1a. Simple Michaelis-Menten kinetics. At low substrate concentration...
When the substrate concentration is such that the reaction =... [Pg.837]

The Michaelis constant is equal to substrate concentration at which the rate of reaction is equal to one-half the maximum rate. The parameters and characterize the enzymatic reactions that are described by Michaelis-Menten kinetics. is dependent on total... [Pg.838]

Figures 11-7 and 11-8 show plots of velocity versus substrate concentration of the interconversion of D-glyceraldehyde 3-Phosphate, and the conversion of urea, respectively. Figures 11-7 and 11-8 show plots of velocity versus substrate concentration of the interconversion of D-glyceraldehyde 3-Phosphate, and the conversion of urea, respectively.
The cell concentration in terms of the substrate concentration and the yield coefficient is ... [Pg.880]

The substrate concentration C is obtained from the Monod expression given by... [Pg.880]

Lineweaver-Burk plot Method of analyzing kinetic data (growth rates of enzyme catalyzed reactions) in linear form using a double reciprocal plot of rate versus substrate concentration. [Pg.904]

Monod kinetics Kinetics of microbial cell growth as a function of substrate concentration proposed by Jacques Monod and widely used to understand growth-substrate relationships. [Pg.905]


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12 - substrates plasma concentration

Biocatalysts substrate concentrations

Bulk substrate concentration

Cellular substrate concentration, enzymes sensitivity

Concentration/time profile substrates

Dependence of Enzyme Reaction Rate on Substrate Concentration

Effect of substrate concentration

Enzyme activity substrate concentration affecting

Enzyme biosensors substrate concentration

Enzyme kinetics substrate concentration variation

Enzyme substrate concentration

Enzymes velocity-substrate concentration curves

Glucose-6-phosphatase substrate concentration

High Substrate Concentration Limit Saturated Kinetics

Hydrogen production high substrate concentration

Hydrogenation kinetic equations, substrate concentration

Inhibition by high substrate concentration

Initial substrate concentration

Initial velocities plotting substrate concentration versus

Inlet substrate concentration

Kinetic Equations and Effect of Substrate Concentration

Kinetic studies substrate concentration

Low substrate concentration

Marker substrate concentration

Nitrification substrate concentration

Outlet substrate concentration

Reaction parameters substrate concentration

Reaction phenotyping substrate concentration, selecting

Reaction rates and substrate concentration

Reaction rates substrate concentration

Reaction substrate concentration

Reaction velocity plotting substrate concentration versus

Substrate (Starting Material) Concentrations

Substrate Concentration Limit Unsaturated Kinetics

Substrate Concentration of Folding Catalysis

Substrate Concentration, Transport into Cells, and Toxicity

Substrate and Product Concentration in Enzymes Following Classical Michaelis-Menten Kinetics

Substrate concentration and rate

Substrate concentration competitive inhibition

Substrate concentration enzymology)

Substrate concentration inside polymer

Substrate concentration relationship

Substrate concentration vapor phase reactions

Substrate concentration, bulk phase

Substrate concentration, dependence

Substrate concentration, effect formation

Substrate concentration, effect response

Substrate concentration, effects

Substrate concentration, effects enzymes

Substrate concentrations normal physiological values

Substrate concentrations relation

Substrate concentrations, influence

Substrates reactions with constant concentration

Velocity vs. substrate concentration

Velocity/substrate concentration curves

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