Apoptosis curcumin

Data about curcunfin encapsulated in liposomes have been reported recently. The authors encapsulated curcumin into a liposomal delivery system in order to study the in vitro and in vivo effects of this compound on proliferation, apoptosis, signaling, and angiogenesis using human pancreatic carcinoma cells. Carotenoids of different polarities and in competition with cholesterol were specifically incorporated into liposomes in order to mimic the physiological uptake by cells and monitor their antioxidant capacities. ... 

The importance of NF-kB to inflammation, apoptosis resistance and tumour progression has resulted in the development of unique NF-kB inhibitors as part of cancer therapeutic regimens for GI and other cancers. Efforts are also being made to understand the efficacy of using natural substances obtained from plants, such as feverfew (e.g. parthenolide), bee glue (e.g. caffeic acid phenylethyl ester), tea (e.g. EGCG), spices (e.g. curcumin from turmeric) and mulberry figs (e.g. morin, a flavone) for the prevention both of persistent NF-kB activation and of the development of inflammatory pre-neoplastic lesions. 

Curcumin represents a covalent inhibitor of CBP. Beyond, it showed anti-angiogenic effects and is currently in phase II/III of clinical trials for cancer therapy, as well as in phase II for Alzheimer s disease and psoriasis [21, 22[. Garcinol, a potent inhibitor of PCAF and p300 (IC50 = 5 and 7 J-M, respectively) was found to induce apoptosis and alter global gene expression in HeLa cells. It was identified to repress chromatin transcription. N one of these inhibitors had effects on H D AC activity or on histone-free DNA transcription [23]. 

Mackenzie GG, Queisser N, Wolfson ML, Fraga CG, Adamo AM, Oteiza PI. 2008. Curcumin induces cell-arrest and apoptosis in association with the inhibition of constitutively active NF-kappaB and STAT3 pathways in Hodgkin s lymphoma cells. Int J Cancer 123 56-65. 

Curcumin has also been found to interrupt the cell cycle, to have cytotoxic effects, and to have a role in antiproliferation and the induction of apoptosis in a hepatocarcinoma cell line. Curcumin is a potent inhibitor of phenol sulfotransferase (SULT1A1) in human liver and extrahepatic tissues [Vietri et al., 2003]. Curcumin inhibited the interleukin-6 (IL-6) production, histone acetyltransferase (HAT) activity, and API activation [Chen et al., 2003a] and prevented cell death and apoptotic biochemical changes, such as the... 

In PC3 cells, curcumin down-regulates MDM2 proteins and mRNA. enhances the expression of the tumor suppressor p21 and inhibits IkBoc [Guo et al., 2006 Li et al., 2007b]. Curcumin can also inhibit prostate cancer via the Akt pathway or the induction of apoptosis by Bcl-2 family members and mitochondrial p53 [Chaudhary and Hruska, 2003 Deeb et al., 2007 Shankar et al., 2007]. 

The in vitro antitumor activity of curcumin in HPV-associated cells has been established [Roy et al., 2002]. Curcumin modulates the in vitro expression and function of Pgp in multidrug-resistant human KB-V1 cells [Anand et al., 2008 Chearwae et al., 2004] and sensitizes cisplatin-resistant SiHa cells to cisplatin-induced apoptosis [Venkatraman et al., 2005], indicating its ability to reverse MDR in cervical cancer cells. The effect of curcumin in HPV-associated cells was found to involve the down-regulation of viral oncogenes, NF-kB and AP-1 [Anand et al., 2008 Divya and Pillai, 2006],... 

Curcumin was found to inhibit cellular proliferation and enhance apoptosis in a variety of lymphoma cell lines in vitro [Skommer et al., 2006 Thompson et al., 2004 Wu et al., 2002]. The proposed mechanism of curcumin s action in the majority of these studies involves the suppression of the expression of NF-KB-regulated gene products. One study suggested a novel function for curcumin as a suppressor of JAK-1 and STAT3 activation in primary effusion lymphoma cells, a function that would lead to the inhibition of proliferation and the induction of caspase-dependent apoptosis [Uddin et al., 2005],... 

In vivo studies using mouse models have proved that curcumin modifies apoptosis resistance, leading to the inhibition of tumor formation and the prevention of adenoma development in the intestinal tract. The chemopreven-tive effect of curcumin for intestinal tumors in Min/+ mice was investigated. A dietary level of 0.15% curcumin decreased tumor formation in Min—/— mice by 63%. Examination of intestinal tissue from the treated animals showed the tumor prevention by curcumin was associated with increased enterocyte apoptosis and proliferation. Curcumin also decreased expression of the oncoprotein (S-catenin in the erythrocytes of the Min/+ mouse, an observation previously associated with an antitumor effect. Curcumin enhanced PhlP-induced apoptosis and inhibited PhIP-induced tumorigenesis in the proximal small intestine of Ape (min) mice. Experiments performed on intestinal tumors in C57BL/6J-Min/+ (Min/+) mice demonstrated that curcumin has a regulatory role in lymphocyte-mediated immune function [Churchill et al., 2000]. Furthermore, levels of COX-2 protein expression have been found to reflect the retardation of adenoma development in mouse intestines after treatment with curcumin [Tunstall et al., 2006]. 

Anto RJ, Maliekal TT, Karunagaran D. 2000. L-929 cells harboring ectopically expressed RelA resist curcumin-induced apoptosis. J Biol Chem 275 15601-15604. 

Atsumi T, Tonosaki K, Fujisawa S. 2006. Induction of early apoptosis and ROS-generation activity in human gingival fibroblasts (HGF) and human submandibular gland carcinoma (HSG) cells treated with curcumin. Arch Oral Biol 51 913-921. 

Atsumi T, Murakami Y, Shibuya K, Tonosaki K, Fujisawa S. 2005b. Induction of cytotoxicity and apoptosis and inhibition of cyclooxygenase-2 gene expression, by curcumin and its analog, alpha-diisoeugenol. Anticancer Res 25 4029—4036. 

Cao J, Liu Y, Jia L, Zhou HM, Kong Y, Yang G. Jiang LP, Li QJ, Zhong LF. 2007a. Curcumin induces apoptosis through mitochondrial hypcrpolarization and mtDNA damage in human hepatoma G2 cells. Free Radic Biol Med 43 968-975. 

Chakraborty S, Ghosh U, Bhattacharyya NP, Bhattacharya RK, Roy M. 2006. Inhibition of telomerase activity and induction of apoptosis by curcumin in K-562 cells. Mutat Res 596 81-90. 

Chen Y, Wu Y, He J, Chen W. 2002. The experimental and clinical study on the effect of curcumin on cell cycle proteins and regulating proteins of apoptosis in acute myelogenous leukemia. J Huazhong Univ Sci Technolog Med Sci 22 295-298. 

Collett GP, Campbell FC. 2004. Curcumin induces c-jun N-terminal kinase-dependent apoptosis in HCT116 human colon cancer cells. Carcinogenesis 25 2183-2189. 

Deeb D, Xu YX, Jiang H, Gao X, Janakiraman N, Chapman RA, Gautam SC. 2003. Curcumin (diferuloyl-methane) enhances tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in LNCaP prostate cancer cells. Mol Cancer Ther 2 95-103. 

Deeb DD, Jiang H, Gao X, Divine G, Dulchavsky SA, Gautam SC. 2005. Chemo-sensitization of hormone-refractory prostate cancer cells by curcumin to TRAIL-induced apoptosis. J Exp Ther Oncol 5 81-91. 

Divya CS, Pillai MR. 2006. Antitumor action of curcumin in human papillomavirus associated cells involves downregulation of viral oncogenes, prevention of NFkB and AP-1 translocation, and modulation of apoptosis. Mol Carcinog 45 320-332. 

Duvoix A, Morceau F, Delhalle S, Schmitz M, Schnekenburger M, Galteau MM, Dicato M, Diederich M. 2003. Induction of apoptosis by curcumin Mediation by glutathione S-transferase PI—1 inhibition. Biochem Pharmacol 66 1475-1483. 

Jiang MC, Yang-Yen HF, Yen JJ, Lin JK. 1996. Curcumin induces apoptosis in immortalized NIH 3T3 and malignant cancer cell lines. Nutr Cancer 26 111-120. 

Jung EM, Lim JH, Lee TJ, Park JW, Choi KS, Kwon TK. 2005. Curcumin sensitizes tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis through reactive oxygen species-mediated upregulation of death receptor 5 (DR5). 

---