Human gingival fibroblasts

In vitro, chlorhexidine can adversely affect gingival fibroblast attachment to root surfaces. Furthermore, protein production in human gingival fibroblasts is reduced at chlorhexidine concentrations that would not affect cell proliferation. Such findings corroborate earlier studies showing delayed wound healing in standardized mucosal wounds after rinsing with 0.5% chlorhexidine solution. 

Atsumi T, Tonosaki K, Fujisawa S. 2006. Induction of early apoptosis and ROS-generation activity in human gingival fibroblasts (HGF) and human submandibular gland carcinoma (HSG) cells treated with curcumin. Arch Oral Biol 51 913-921. 

Atsumi T, Fujisawa S, Tonosaki K. 2005a. Relationship between intracellular ROS production and membrane mobility in curcumin- and tetrahydrocurcumin-treated human gingival fibroblasts and human submandibular gland carcinoma cells. Oral Dis 11 236-242. 

Takahashi A, Ikehara T, Hosokawa K, et al. 1995. Properties of Ca2+-dependent K+ channels of human gingival fibroblasts. J Dent Res 74(8) 1507-1512. 

Normal cells used in this study are human gingival fibroblast (HGF), pulp cell (HPC), and periodontal ligament fibroblast (HPLF). Tumor cell lines we... 

Stromelysin was isolated as the zymogen from the culture media of interleukin-1-stimulated human gingival fibroblasts. The zymogen is activated by incubating with trypsin, which in turn is inactivated by adding soybean trypsin inhibitor. 

Lefeuvre M, Amjaad W, Goldberg M, Stans lawk i L. TEGDMA induces mitochondrial damage and oxidative stress in human gingival fibroblasts. Biomaterials 2005 26 5130-7. 

Cestrum noctumum spirostane (36) HSC-2 (oral squamous carcinoma), HGF (human gingival fibroblast) LD50 2.0-2.8 lig/ml [29]... 

Yang Q, Nakkula RJ, Walters JD, Accumulation of ciprofloxacin and minocycline by cultured human gingival fibroblasts, J. Dent. Res. 2002 81 836-840. 

Human gingival fibroblasts (HGF) were maintained according to the method of Elvin and Evans [19]. Stock cultures were recovered from liquid nitrogen and plated at 200,000 cells per 25 cm tissue-culture flask in a-DMEM with 10% foetal bovine serum and 1% antibiotics. 

Fig. 3. (A) SEM images of different positions (sand blasted (sb), 1, 4 and 9mm) and the according roughness value Ra on the roughness gradient. Morphology of rat calvarial osteoblasts (B) and human gingival fibroblasts (C) at different positions on the gradient. Cells were cultured for 7 days. The nucleus was stained with DAPI (blue) and the actin network with Alexa Fluor 488 phalloidin (green). Scale bar is 200 pm.

Kononen M, Hormia M, Kivilahti J, Hautaniemi J, Thesleff I. Effect of surface processing on attachment, orientation, and proliferation of human gingival fibroblasts on titanium. J Biomed Mater Res 1992 26 1325-41. 

Tsai CH, Yang SF, Huang FM, Chang YC. The upregulation of cystatin C in human gingival fibroblasts stimulated with cyclosporine A. J Periodontal Res 2009 44(4) 459-64. 

Normal human oral keratinocytes, human gingival fibroblasts, and human epidermal keratinocytes. 

Human oral keratinocytes derived from gingival tissue and primary normal human gingival fibroblasts. 

It has been shown that tannic acid (TA) exerts cancer chemopreventive activity in various animal models (61). TA induced either growth arrest or apoptotic death (62). TA induced apoptosis more in human oral squamous cell carcinoma and salivary gland tumor cell lines than in normal human gingival fibroblasts, whereas gallic acid, a component unit of TA, showed much weaker selective cytotoxicity (63). Recently, it was shown that inhibition of the proteosome of living Jurkat cells results in accumulation of two natural proteosome substrates, the cyclin-dependent kinase inhibitor p27 and the proapoptotic protein Bax, followed growth arrest in G1 and induction of apoptotic cell death (d ). 

Chang, Y. C. Chou, M. Y. Cytotoxicity of halothane on human gingival fibroblast cultures in vitro. J. Endod. 2001, 27, 82-84. 

Argentin, G. Cicchetti, R. Genotoxic and antiapoptotic effect of nicotine on human gingival fibroblasts. Toxicol. Sci. 2004, 79, 75-81. 

---