Gene global

Gene Expression Analysis. Figure 1 Schematic representation of the experimental process of global gene expression analysis using Affymetrix GeneChip arrays. 

Classical global knockouts may have a developmental or lethal phenotype and thus preclude the analysis of the phenotypic consequences of the lack of a gene in specific tissues in adult animals. With the development of the cre/loxP and flp/FRT systems, it has become possible to excise defined DNA fragments from the genome of specified cells. Cre and Flp are bacterial and yeast recombinases, respectively, which recognize loxP and FRT sequences, respectively. The most common... 

Luo W, Fan W, Xie H, ling L, Ricicki E, Vouros P, et al. Phenotypic anchoring of global gene expression profiles induced by A -hydroxy-4-acetylaminobiphenyl and benzo[a]pyrene diol epoxide reveals correlations between expression profiles and mechanism of toxicity. Chem Res Toxicol 2005 18 619-29. 

Proteomics, the measurement of the global changes in proteins produced as a result of gene expression, bridges the gap between genome sequence and cellular behaviour and takes into account the post-translational modifications that often result in the functional effect. It has the potential to determine the role of protein-protein complexes in the complex signalling cascades that... 

Wang, A. and Crowley, D.E., Global gene expression responses to cadmium toxicity in Escherichia coli, J Bacteriol, 187 (9), 3259-3266, 2005. 

---