Ames mutagen assay, mutagenic

Hughes TJ, Simmons DM, Monteith LG, et al. 1987. Vaporization technique to measure mutagenic activity of volatile organic chemicals in the Ames/ Salmonella assay. Environ Mutagen 9 421-441. 

Cyclopentane was not mutagenic in the Ames bacterial assay it was also negative in vitro in the mouse lymphoma assay and in the micronucleus test. ... 

No evidence of mutagenicity was seen in Ames bacterial assays. Diethylamine has an ammonia-like odor that is detectable at... 

Picric acid was mutagenic in the Ames Salmonella assay in the presence of metabolic activation. ... 

Negative results were reported in various mutagenic assays including the Ames Salmonella assay (with or without microsomal activation), sister chromatid exchange assay in mouse lymphoma cells, mouse bone marrow cyto-genic analysis, and mouse dominant lethal assay ... 

MBT was not mutagenic in Ames bacterial assays, but it induced chromosomal damage in mammalian cells in culture. ... 

Until the early to mid-1980s, research on the mutagenicity of respirable POM focused almost exclusively on the particulate phase. Another aspect of tropospheric chemistry with significant health implications is the application of the Ames bacterial assay (with or without the microsuspension modification) to the detection and identification of mutagenic vapor-phase PAHs and PACs. For example, Harger and co-workers (1992) reported that the mutagenicities of concurrently collected samples of vapor-phase and particle-phase organics in southern California (Claremont, California) ambient air were comparable. 

As with the Salmonella reversion assay, this shortterm test is conducted both without (— PMS) and with metabolic activation produced by addition of post-mitochondrial supernatant containing rat liver enzymes ( + PMS). These terms are equivalent to — S9 and + S9 in the Ames reversion assay we use the latter designation for both types of bacterial assays. A more sensitive micro-forward mutation bioassay using this TM677 strain to determine the mutagenicity of indoor air particles, including ETS and wood smoke, is described by Lewtas et al. (1987). 

Stark and co-workers (1985) reported that irradiation of a 0.1 mM solution of 1-nitropyrene in 2-propanol with light from 320 to 418 nm changes its absorption spectrum and concurrently results in almost total loss of its direct ( —S9) or activatable ( + S9) mutagen activity in the Ames Salmonella assay. 

Hughes, T. J., D. M. Simmons, L. G. Monteith, and L. D. Claxton, Vaporization Technique to Measure Mutagenic Activity of Volatile Organic Chemicals in the Ames/Salmonella Assay, Environ. Mutagen., 9, 421-441 (1987). 

Lofroth, G, Nilsson, L. Andersen, J.R. (1986) Stracture-activity relationship of nitroalkane-induced mutagenicity in the Ames Salmonella assay. Genetic toxicology of environmental chemicals, Part B Genetic effects and applied mutagenesis. Prog. din. biol. Res., 209B, 149-155... 

Weinstein, D.. Katz, M. Kazmer, S. (1981) Use of a rat/hamster S-9 mixture in the Ames mutagenicity assay. Environ. Mutag., 3. 1-9... 

The modified Ames mutagen assay was used to investigate the mutagenic potential of nitrosated fru-trp, a fru-trp control, and a nitrite control incubated in solutions under mild conditions (37°C, pH 4, 24 h). Figure 1 shows dose response curves obtained with S. typhimurium TA98 and TA100. Nitrosated fru-trp demonstrated a positive dose-response relationship with both tester strains in the absence of S-9 mix and a somewhat reduced dose-response relationship in its presence. The fru-trp and nitrite controls did not manifest mutagenic properties in either tester strain. 

Hye-Won A, Jeffrey EH. 1994. Effect of aluminum on fluoride uptake by salmonella typhimurium TA98 implications for the Ames mutagenicity assay. J Toxicol Environ Health 41 357-368. 

Noot D. K., Anderson W. B., Daignault S. A., Williams D. T., and Huck P. M. (1989) Evaluating treatment processes with the Ames mutagenicity assay. J. Am. Water Works Assoc. 81, 87-102. 

Three guaiane sesquiterpenes, the ester (4.13), lactaroviolin (4.3), and deterrol (4.7) showed a weak mutagenic activity in the Ames Salmonella assay (137). In addition, deterrol (4.7) exhibited moderate cytotoxicity towards Ehrlich ascitic tumor cells (EGA cells) and weak toxicity towards L1210 cells (lymphocytic leukaemia mouse) (137). Lactaroviolin (4.3) showed a weak inhibitory effect towards EGA cells, while the ester 4.13 did not impair either cell line at 50 Xg/ml (137). The Swedish authors pointed out that the greater toxicity of deterrol than of lactaroviolin was rather... 

Cote C, Blaise C, Delisle CE, et al. A miniaturized Ames mutagenicity assay employing bioluminescent strains of Salmonella typhimurium. Mutat Res. 1995,345(3-4) 137-146. 

Caffeine has been found to be weakly mutagenic in some nonmammalian animal models. It is not been found to be mutagenic in Ames Salmonella assays. 

N-Acetylcysteine is negative in the Ames mutagenicity test and also reduces the mutagenic affect of chemical carcinogens in the same assay. 

Chlorpropamide was mutagenic in Chinese hamster cells but negative in Ames Salmonella assays. 

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