Sister chromatid exchange assay

Purpose. The purpose of the sister chromatid exchange (SCE) assay is to evaluate the potential of the test substance to induce repair of DNA lesions by homologous recombination in cells of treated animals (potentially all species, usually rodents) (Latt et al. 1981 Helleday 2003). It can easily be applied to any dividing tissue, such as bone marrow and peripheral blood, from which cell suspensions can be isolated and analyzed. [Pg.326]

Study Design. Chromatids can be visualized in the late prophase and early metaphase of mitosis, before chromatid segregation in daughter cells. In order to increase [Pg.326]

Assay Limitations and Interpretation. Incorporation of BrdU itself can contribute to SCE, because it results in single strand-breaks and alkali-labile sites. Immunofluorescence methods that only necessitate small doses of BrdU for SCE visualization yield a lower SCE background than other techniques (Wilson and Thompson 2007). A method using Biotin-dUTP instead of BrdU was recently reported to overcome this technical issue (Wojcik et al. 2004). [Pg.327]

OECD 481] In vitro sister chromatid exchange assay [OECD 479] / ... [Pg.192]

Genetic Toxicology In Vitro Sister Chromatid Exchange Assay in Mammahan Cells (Original Guideline, adopted 23 October 1986)... [Pg.21]

The compound was not mutagenic in bacterial assays or genotoxic in sister chromatid exchange assays but was immunotoxic, decreasing the rate of lymphocyte proliferation in... [Pg.242]

Isophorone does not induce gene mutations in bacteria, chromosomal aberrations in vitro, DNA repair in primary rat hepatocytes, or bone marrow micronuclei in mice. Positive effects were observed only in the absence of an exogenous metabolic system in L5178YTK+/— mouse mutagenesis assays as well as in a sister chromatid exchange assay. The weight of evidence of all mutagenicity data supports the contention that isophorone is not a potent DNA-reactive compound. ... [Pg.410]

Negative results were reported in various mutagenic assays including the Ames Salmonella assay (with or without microsomal activation), sister chromatid exchange assay in mouse lymphoma cells, mouse bone marrow cyto-genic analysis, and mouse dominant lethal assay ... [Pg.607]

Titton Bionetics. 1980a. Sister chromatid exchange assay, Ames assay, mouse lymphoma forward mutation assay, and transformation assay for a sample containing 33-1/3% each ortho-, meta-, and para-cresol. Unpublished data submitted to EPA/OTS. Fiche no. OTS0517528. [Pg.154]

Natarajan, A.T. van Kesteren-van Leeuwen, A.C. (1981) Mutagenic activity of 20 coded compounds in chromosone aberrations/sister chromatid exchanges assay using Chinese hamster ovary (CHO) cells. In de Serres, F.J. Ashby, J., eds. Evaluation of Short-Term Tests for Carcinogens. Report of the International Collaborative Program (Progress in Mutation Research, Vol. 1), Amsterdam, Elsevier, pp. 551-559... [Pg.541]

Antimony trioxide was genotoxic in a number of older bacterial mutation assays but not in more recent studies. Positive results were observed with antimony trioxide in the in vitro cytogenetic assay with human lymphocytes and the sister chromatid exchange assay with V79-cells, but not in the L5178Y mutation assay. [Pg.151]

Ethyl acetate induced aneuploidy in Sac-charomyces cerevisiae. It yielded positive results in an in vitro sister chromatid exchange assay in Chinese hamster ovary cells. In vitro chromosomal aberration assays were positive in Chinese hamster fibroblast cells and negative in Chinese hamster ovary cells. (An in vivo bone marrow micronucleus study in Chinese hamsters yielded negative results by both intraperitoneal and oral administrations.)... [Pg.1089]

Mitomycin C is a carcinogen (sarcomas and other cancers) in mice and rats after intraperitoneal, intravenous, or subcutaneous injections. It is teratogenic in mice (including skeletal defects). It is nephrotoxic. It is mutagenic in sister chromatid exchange assays. [Pg.1703]

OECD (1986) Genetic Toxicology in vitro Sister Chromatid Exchange Assay in Mammalian Cells. OECD Guideline for Testing of Chemicals 479. Organization for Economic Cooperation and Development, Paris. [Pg.254]

DNA Adducts Unscheduled DNA Synthesis Assay in Liver Cells Sister-Chromatid Exchange Assay Gene Mutation Assays... [Pg.301]

A 13-week inhalation study in rats sponsored by the National Toxicology Program (NTP) has been completed, but the results were not published as of March 1996. The NTP has also reported that an inhalation carcinogenicity study in rats has been planned, but no details on this study are available. Preliminary reports on NTP-funded genotoxicity studies indicate positive results in a sister chromatid exchange assay (in vitro) and negative results in both a chromosome aberration assay (in vitro) and in two Salmonella tests. No further data on these studies were available. [Pg.124]

In vitro Sister Chromatid Exchange Assay in Mammalian Cells... [Pg.586]

Genotoxicity Bacterial reverse mutation (Ames) Sister chromatid exchange assay Bacteria Mouse lymphoma L5178Y cells 95% confidence, control = sample 95% confidence, control = sample... [Pg.344]

Chromosomal aberrations Test 479, in vitro sister chromatid exchange assay in mammalian cells. [Pg.14]

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