Monocytic cell line

Umehara H, Goda S, finai T, et al. Fractalkine, a CX3C-chemokine, functions predominantly as an adhesion molecule in monocytic cell line THP-1. Immunol Cell Biol 2001 79(3) 298-302. 

Graziani-Bowering G, Filion LG, Thibault P, Kozlowski M. CD4 is active as a signaling molecule on the human monocytic cell line Thp-1. Exp Cell Res 2002 279(1) 141-152. 

The study of osteoclast differentiation is important for understanding potential new treatments for osteoporosis. Such therapies are typically explored in tissue culture models such as the Raw264 mouse monocytic cell line, which is capable of differentiation into functional multinuclear osteoclasts after treatment with the cytokine Rankl. Use of transformed cell lines raises the concern that results may not be extrapolated to normal tissue. To address this question, the transcriptional responses for Rankl treatment of the Raw264 cell line, and of two ex vivo primary cell systems (bone marrow macrophages, and hematopoetic stem cells) were compared using Affymetrix GeneChips [23]. The models proved to... 

Aupeix, K, Weltin, D, Mejia, JE, Christ, M, Marchal, J, Freyssinet, JM, Bischoff, P., 1995, Oxysterol-induced apoptosis in human monocytic cell lines. Immunobiology 194 15-28. 

Berg, J., Christoph, T., Widerna, M., Bodenteich, A. Isoenzyme-specific cyclooxygenase inhibitors a whole cell assay system using the human erythroleukemic cell line HEL and the human monocytic cell line Mono Mac 6, J. Pharmacol. Toxicol. Methods 1997, 37, 179-186. 

We have observed similar findings using U937, a monocytic cell line, and have detected EMP-monocyte complexes in patients with MS [67]. 

Recruit volunteers who met entrance requirements 2. Screen botanical ingredients for inhibition of IL-1 p production 3. Pilot clinical evaluation of IL-1 inhibitory lead candidate botanicals from activity 2 Establishing a clinical genotyped database Human monocyte cell lines stimulated in vitro with LPS Clinical + laboratory assay of peripheral blood mononuclear cells (PBMCs), and plasma Adequate number of healthy subjects with CRP = 2-10 mg/L and stratified by IL1 composite genotype Select lead candidate botanicals based on IL-1 protein inhibitory dose compared to untreated cultures IL1 gene expression in PBMCs and ex vivo IL-1 production in plasma from test subjects after 2-week dosing of candidate botanicals... 

Recently, Sedqi et al. [24] were able to clone a delta opioid receptor complementary DNA by expression of cDNA library from activated thymocytes in Cos 7 cells, whose amino acid sequence was similar to the neural counterpart. Interestingly, they also observed that transcripts for kappa and mu opioid receptors were not detected in thymocytes. Furthermore, Gave-riaux et al. [25] demonstrated transcripts for the delta opioid receptor in T-lymphocyte, B-lymphocyte, and monocyte cell lines, as well as in murine splenocytes. However, they observed that the kappa opioid receptor transcript was only found in B-cell lines. These studies may suggest a selective expression of the delta opioid receptor in specific cells and tissues of the immune system and suggests specialized functions in different anatomical regions. 

Oxysterol-induced apoptosis in human monocytic cell lines. Immunobiol. 194, 415-428. Bascoul, J., Domergue, M., Mourot, J., Dery, G., Crastes de Paulet, A. 1986. Intestinal absorption and fecal excretion of 5,6-a-epoxy- 5a-cholesta-3 (3-ol by the male wistar rat. Lipids 21, 744-747. 

Hisaka H, Kataoka M, Higuchi Y, Matsuura K, Yamamoto S. Close localization of mouse CD14 and CD32/16 in the cell surface of monocytic cell lines. Pathobiology 1999 67(2) 92—8. 

RPR103611 (246) was tested in a panel of isolates and cell lines, including the monocytic cell line U-937 and peripheral blood lymphocytes. The IC50 values... 

Israel, N. et al. The redox status of cells influences constitutive or induced NFkB translocation and HIV-LTR activity in human T and monocytic cell lines, J. Immunol., 149, 3386, 1992. 

C. Rafferty, B. Poole, S. Assay of pyrogens by interleukin- 52. 6 release from monocytic cell lines. Pharm. Pharmacol. 

Clark, J. D., Milona, N., and Knopf, J. L. (1990). Purification of a 110-kilodalton cytosolic phospholipase A2 from the human monocytic cell line U937. Proc. Natl. Acad. Sci. U.S.A. 87, 7708-7712. 

Friedland, J.S., Remick, D.G., Shattock, R. and Griffin, G.E. (1992). Secretion of interleukin-8 following phagocytosis of Mycobacterium tuberculosis by human monocyte cell lines. Eur. J. Immunol. 22, 1373-1378. 

Bone tissue also contains osteoclasts, which are multinucleated cells that are derived from the hematopoietic (granulocyte-monocyte) cell line located in bone marrow. Osteoclasts are primarily responsible for bone resorption and they secrete enzymes and hydrochloric acid that break down collagen matrix and help dissolve the bone. The area where osteoclast cell membrane lies adjacent to bony tissue is known as a Howship s lacunae. The osteoclast cell membrane that lies in close proximity to bone can contain numerous villous extensions and form a ruffled border. These areas are also known as resorption pits. The plasmalemma border of the osteoclast cell in this region forms a specialized seal with the underlying bone to prevent the release of enzymes and hydrochloric acid. This process also results in the release of growth factors previously deposited in bone by osteoblasts, which are responsible for maintaining the process of regenerating new bone. 

Numerous agents control mesenchymal stem cell derivation and differentiation of osteoblasts (Fig. 88-2). Adipocytes can be converted to osteoblasts under the control of Runx2 and peroxisome proliferator-activated receptor-y 2 (PPAR y 2). Osteoclasts are derived from the myeloid/monocyte cell line with derivation and differentiation also under the control of numerous agents, some of which are expressed from osteoblasts. ... 

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