Suicide gene

Vascular gene therapy Transfer of dominant-negative receptors or suicide genes under the control of angiogenic endothelial cell specific promoters... 

Degrfeve B, De Clercq E, Balzarini J (1999) Bystander effect of purine nucleoside analogues in HSV-1 tk suicide gene therapy is superior to that of pyrimidine nucleoside analogues. Gene Ther 6 162-170... 

DeFatta, R., Li, Y., and De Benedetti, A. (2002). Selective killing of cancer cells based on translational control of a suicide gene. Cancer Gene Ther. 9, 573-578. 

Inserting toxin genes in tumour cells in order to promote tumour cell destruction Inserting suicide genes into tumour cells... 

Tanaka, M., N. Inase, S. Miyake, and Y. Yoshizawa. 2001. Neuron specific enolase promoter for suicide gene therapy in small cell lung carcinoma. Anticancer Res 21(lA) 291-4. 

C. J. Springer, I. Niculescu-Duvaz, Prodrug-Activating Systems in Suicide Gene Therapy , J. Clin. Invest. 2000,105, 1161-1167. 

Anderson DG, Peng W, AMnc A, Hossain N, Kohn A, Padera R, Langer R, SawicM JA (2004) A polymer library approach to suicide gene therapy for cancer. Proc Natl Acad Sd USA 101 16028-16033... 

D. Nafziger, J. Pegg, D. Paielli, S. Brown, K. Barton, M. Lu, E. Aguilar-Cordova, J.H. Kim, Phase I study of replication-competent adenovirus-mediated double suicide gene therapy for the treatment of locally recurrent prostate cancer. Cancer Res. 62 (2002) 4968-4976. 

T. Dresselaers, J. Theys, L. Dubois, W. Landuyt, P. Van Hecke, P. Lambin, Evaluation of salmonella-based suicide gene transfer in a rodent tumor model using in vivo 19F MR spectroscopy. Proc. Inti. Soc. Mag. Reson. Med. (2006) p. 3175. 

In 1991, several factors were demonstrated to affect the efficiency of relE controlled killing of E. colt when under lac promoter control (Knudsen Karlstrom, 1991)- Cells escaped suicide primarily because of the mutation rate and the leakiness of repression during normal growth. When relF was under lac JV5 promoter/operator control, the inactivation of suicide function through spontaneous mutation occurred at a frequency of <5 x 10 9. Knudsen has further theorized that if the number of suicide minus (mutant) bacteria in a culture can be kept at zero before induction, all cells will die. This can be achieved in two ways provide the suicide function in duplicate, and control the suicide gene expression so stringently, with a chromosomally located laclq gene, that a basal level of the suicide function to which cells can adapt will not be present (Knudsen et al., 1995). 

Hajri, A., Wack, S., Lehn, P., Vigneron, J.P. and Lehn, J.M. (2000) Efficient transfer of double suicide genes (herpes simplex virus-thymidine kinase and Escherichia coh-CD) into peritoneal disseminated pancreatic tumor cells by the cationic lipid BGTC. Cancer Gene Ther., 7,1393-1393. 

Springer, C. J., and Niculescu-Duvaz, I. Prodrug-activating systems in suicide gene therapy. J. Clin. Invest. 105 1161-1167, 2000. 

Fillat, C., Carrio, M., Cascante, A., and Sangro, B. Suicide gene therapy mediated by the herpes simplex virus thymidine kinase gene/ganciclovir system Fifteen years of application. Curr. Gene. Ther. 3 13-26, 2003. 

Y. Kawashita, A. Ohtsuru, Y. Kaneda, Y. Nagayama, Y. Kawazoe, S. Eguchi, H. Kuroda, H. Fujioka, M. Ito, T. Kanematsu, and S. Yamashita, Regression of hepatocellular carcinoma in vitro and in vivo by radiosensitizing suicide gene therapy under the inducible and spatial control of radiation, Hum. Gene Ther. 10 1509 (1999). 

S. O. Freytag, K. R. Rogulski, D. L. Paielli, J. D. Gilbert, and J. H. Kim, A novel three-pronged approach to kill cancer cells selectively concomitant viral, double suicide gene, and radiotherapy, Hum. Gene Ther. 9 1323 (1998). 

O. Wildner, J. C. Morris, N. N. Vahanian, H. J. Ford, W. J. Ramsey, and R. M. Blaese, Adenoviral vectors capable of replication improve the efficacy of HSVtk/ GCV suicide gene therapy of cancer, Gene Ther. 6 51 (1999). 

G. Cao, S. Kuriyama, J. Gao, A. Mitoro, L. Cui, S. Nagao, X. Zhang, H. Tsujinoue, X. Pan, H. Fukui, and Z. Qi, In vivo gene transfer of a suicide gene under the transcriptional control of the carcinoembryonic antigen promoter results in bone marrow transduction but can avoid bone marrow suppression, Int. J. Oncol. 15 107 (1999). 

G. Gazit, G. Hung, X. Chen, W. F. Anderson, and A. S. Fee, Use of the glucose starvation-inducible glucose-regulated protein 78 promoter in suicide gene therapy of murine fibrosarcoma, Cancer Res. 59 3100 (1999). 

R. V. Blackburn, S. S. Galoforo, P. M. Corry, and Y. J. Lee, Adenoviral-mediated transfer of a heat-inducible double suicide gene into prostate carcinoma cells, Cancer Res. 58 1358 (1998). 

Steg GR Tahlil O. Aubailly N, et al. Reduction of restenosis after angioplasty in an atheromatous rabbit model by suicide gene therapy, Circulation 1997 96 408-41 I. 

Molin, S. (1993), Environmental potential of suicide genes, Curr. Opin. Biotechnol., 4, 299-305. 

Figure 4. A suicide gene expression containment model system. PI - Promoter of Inducible operon. VP2 - Viral promoter 1. VP2 - Viral promoter 2.

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