Stereotaxic surgery

Key words Lentivirus, RNA interference, stereotaxic surgery, mouse models, drug addiction. 

Marsden, C., Obeso, J. (1994). The functions of the basal ganglia and the paradox of stereotaxic surgery in Parkinson s disease. Brain, 117, 877-897. 

With the early application of stereotaxic surgery to neurological studies in experimental animals, Anand and Brobeck (1951) first reported that bilateral lesions of small nuclei in the hypothalamus could disrupt feeding behavior with medial hypothalamic lesions causing obesity and lateral lesions aphagia. In the latter syndrome, the lesioned animals would completely stop eating to the extent that if not maintained... 

Paxinos, G., Watson, C., Pennisi, M., and Topple, A. (1985). Bregma, lambda and the interaural midpoint in stereotaxic surgery with rats of different sex, strain and weight. J. Neurosci. Meth. 13, 139-143. 

Stereotaxic surgery set up with frame, cannulae, injection units, drill, and surgical materials. 

Watanabe, E., T. Watanabe, and S. Manka, et al., Three-dimensional digitizer (neuronavigator) New equipment for computed tomography-guided stereotaxic surgery, Surg. Neurol., 1987, 27 543-547. 

Values are expressed as mean glucose utilization (p.mol/100 g/min) SEM. Sham animals (n = 7) had been subjected to intrastriatal injections of 2 (il of CSF 10 days prior to measurement of local cerebral glucose utilization. Lesioned animals (n = 6) had been subjected to intrastriatal injections of 2 p.g of kainic acid 10 days prior to the measurement of local cerebral glucose utilization. Sham-lesioned animals are significantly lower in every region, except in internal capsule, from control animals (n = 9) in which no stereotaxic surgery was performed. 

Dial test indicator (David Kopf, Model 1910). This tool is used to level the micro-manipulator arm with respect to the base unit during setup of the stereotaxic alignment system, according to the manufacturer s instructions. It is not required during actual surgery. 

Stereotaxic coordinates for injection into brain regions other than those listed in Table 17.1 should be calculated from a mouse brain atlas (9). Prior to surgery, use the atlas to determine optimal coordinates for injection in... 

Alksne and Smith described the novel use of an iron acrylic compound for the stereotaxic thrombosis of intracranial aneurysms in patients who were poor risk candidates for surgery. The compound polymerized rapidly, did not fragment and was non-toxic. Good results were reported in experimental animals and humans. 

Guetrouad, A., and P. Vidal, S.M.O.S. Stereotaxical Microtelemanipulator for Ocular Surgery, Proc. cf the Annual Ini I Conf. of the IEEE Engineering in Medicine and Biology Society, 1989,11 879-880. 

For surgery 20 control females were anaesthetized at the age of 165 days with an intraperitoneal injection of 45 mg/kg of ketamine. Ibotenic acid (IBO) was stereotaxically injected into the dorsal hippocampi (10 jUg IBO in 0.5 jul of phosphate buffer into each hemisphere). Ten additional females were treated alike without IBO injection (sham operation). For amygdala lesions 0.4 jUg IBO in 0.4 jul phosphate buffer were stereotaxically injected into each side. 

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