Stem Cell Isolation

Intracellular Enzymes Stem Cell Isolation Cellular Membrane Potential Mitochondrial Membrane Potential Gene Reporter Assay Gene Silencing (siRNA)... 

The ability of adult stem cells to become something other than what they were destined to be is controversial. One team of scientists has reported that a particularly promising adult stem cell, isolated from bone marrow and called a multipotent adult progenitor cell (MAPC), appears able to develop into many different kinds of specialized cells in the laboratory. Other scientists have not been able to reproduce these results. Additionally, scientists have reported that stem cells found in fat can become muscle cells, nerve cells, or even pancreas cells able to make insulin, under the right laboratory conditions. 

Dr. Alain Fisher and his research team have successfully treated two infants with XSCIDS, a severe form of SCIDS that occurs only in boys. These patients lack functional T cells and natural killer cells (NK) due to mutations in the % chain of the cytokine receptor family that recognizes interleukins (i.e., IL-2, -4, -7, -9, and -15). Ex vivo gene transfer was employed. The researchers delivered the wild-type sequence for the yc chain cytokine receptor subunit to hematopoietic stem cells isolated from these patients using a nonreplicating murine retrovirus [14,15]. 

Imura, T., Komblum, H.I., Sofroniew, M.V. (2003). The predominant neural stem cell isolated from postnatal and adult forebrain but not early embryonic forebrain expresses GFAP. JNeurosci, 23, 2824-32. 

EGF-responsive neural stem cells isolated from rat and mouse brain are capable of differentiating into oligodendrocytes and of forming myelin following transplantation into the myelin deficient rat. Duncan, I.D., Archer, D.R., Hammang, J.P. (1993). Abstr Soc Neurosci, 19 (1-3) Abs 689. 

Stem cell isolation and expansion BM aspirates were combined with DPBS and centrifuged several times at different density gradients to collect hMSCs. Cells were then resuspended in hMSC complete culture medium and plated at 3 x 107 cells/185 cm2. The cultures were maintained at 37°C in a humidified atmosphere before being passaged prior to confluency. 643694"... 

Phenotype of human mesenchymal stem cells isolated directly from bone marrow Davis-Sproul, J., McNeil, R., Simonetti, D., Craig, S., Moseley, A., Deans, R., Moorman, M. (1999). Blood, 94 10 Suppl 1 Abs 3905. 

Mesenchymal stem cells Isolation, in vitro expansion and characterization Beyer, N.N., da Silva, M.L. (2006). Handb Exp Pharmacol, 174 249-282. 

Rietze RL, Reynolds BA (2006) Neural stem cell isolation and characterization. Methods Enzymol 419 3-23. 

Cell therapy is a potential therapeutic approach for AD. Tricyclodecan-9-yTxanthogenate (D609) was used to induce human mesenchymal stem cells isolated from Wharton jelly of the umbilical cord (HUMSCs) to differentiate into neuron-like cells... 

Stem cells are defined by two major criteria - pluripotentahty and self-renewal capacity. Recent developments in the field of stem cell research indicate their enormous potential as a source of tissue for regenerative therapies. The success of such apphcations will depend on the precise properties and potentials of stem cells isolated either from embryonic, fetal or adult tissues. 

Mesenchymal stem cells isolated from murine bone marrow were applied in a study designed to evaluate the molecular toxicity of hydroxyapatite nanoparticles (Remya et al., 2014). Hydroxyapatite nanoparticles (50 nm) were used to study the cytotoxicity, nanoparticle uptake, effect on cytoskeletal arrangement, oxidative stress response and apoptotic behaviour with the confluent cells as per standard protocols. The results of the MTT assay indicated that hydroxyapatite nanoparticles do not induce cytotoxicity up to 800 pg ml-1. It was also observed that apoptosis related to oxidative stress and reactive oxygen species (ROS) production following nanoparticle treatment was comparable to that of the control (cells without treatment). Hence, it can be concluded that mesenchymal stem cell in vitro cultures can be used as a model to evaluate the potential toxicity of nanomaterials. 

Zulewski H, Abraham E J, Gerlach M J, et al. (2001). Multipotential nestin-positive stem cells isolated from adult pancreatic islets differentiate ex vivo into pancreatic endocrine, exocrine, and hepatic phenotypes. Diabetes. 50 521-533. 

Gang E J, Jeong J A, Hong S H, et al. (2004). Skeletal myogenic differentiation of mesenchymal stem cells isolated from human umbilical cord blood. Stem Cells. 22 617-624. 

UG-MSG Mesenchymal stem cells isolated from umbilical cord umcl Unmodified/raw medium-chain-length... 

Microfluidic stem cell culture Stem cell isolation Stem cell reprogramming Stem cell tissue engineering... 

Stem cells isolated from human exfoliated deciduous teeth (SHED) have been identified as a novel population of MSC family. SHED have the same capacity as bone marrow-derived MSCs to differentiate into multiple cell types, including osteoblasts and odontoblasts, and to generate bone or dentin-pulp complex tissue (Miiua et al., 2003 Cordeiro et al., 2008 Yamada et al., 2010). However, osteogenic/odontogenic differentiation of SHED and bone marrow-derived MSCs is regulated by different mechanisms, and SHED can have a stronger tendency to be induced into odontoblasts than bone marrow-derived MSCs (Kara et al., 2011). Most importantly, SHED show very strong proliferative abilities and can be obtained from deciduous teeth without any ethical concern, which may provide a useful cell source as an alternative to bone marrow-derived MSCs for mineralized tissue formation. 

Stem cells, which makes it a potential source of allogeneic multipotent cells for bone healing.AM stem cells isolated have the capacity for in vitro osteogenesis, which makes them a potential source of allogeneic stem cells for application in bone reconstruction. ... 

NP-Based Gene Delivery for Stem Cell Isolation and Culture... 

Jackson KA et al (1999) Hematopoietic potential of stem cells isolated from murine skeletal muscle. Proc Natl Acad Sci USA 96 14482-14486... 

Lee M-J, et al. Anti-fibrotic effect of chorionic plate-derived mesenchymal stem cells isolated from human placenta in a rat model of CC14-injured Ever potential appE-cation to the treatment of hepatic diseases. J Cell Biochem 2010 111 1453-63. 

Eberhardt, M., Salmon, R, Von Mach, M. A., Hengstler, J. G., Brulport, M., Linscheid, R, Seboek, D., Oberholzer, J., Barbero, A., Martin, 1., MuUer, B., Trono, D., and Zulewski, H. 2006. Multipotential nestin and Isl-1 positive mesenchymal stem cells isolated from human pancreatic islets. Biochem... 

Puetzer, J.L., J.N. Petitte, and E.G. Loboa, Comparative review of growth factors for induction of three-dimensional in vitro chondrogenesis in human mesenchymal stem cells isolated from bone marrow and adipose tissue. Tissue Eng Part B Rev, 2010.16(4) 435-44. 

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