Spectrophotometer Varian Cary

Co concentration was determined by spectrophotometer (Varian Cary 500) at 692 nm wave length, with the sample diluted with a 9 mol/L concentrated HCl solution. NO content in gas phase was obtained by an on-line Fourier transform infrared spectrometer (Nicolet E.S.P. 460 FT-IR) equipped with a gas cell and a quantitative package, Quant Pad. 

All spectroscopic studies were carried out with UV-vis spectrophotometer Varian Cary 100 at constant agitation and thermostatic control at 20°C. 

The absorbents prepared were tested for their behavior in the desulfurization of gas streams containing H2S. The gas flow was analyzed using a UV-VIS spectrophotometer, Varian Cary 1. The H9S concentration was measured at 232 nm and the SO9 concentration at 262 nm. 

Ultraviolet-visible (UV-vis) diffuse reflectance spectra of supported WOx samples and standard W compounds were obtained with a Varian (Cary 5E) spectrophotometer using polytetrafluoroethylene as a reference. The Kubelka-Munk function was used to convert reflectance measurements into equivalent absorption spectra [12]. Spectral features of surface WOx species were isolated by subtracting from the W0x-Zr02 spectra that of pure Z1O2 with equivalent tetragonal content. All samples were equilibrated with atmospheric humidity before UV-vis measurements. 

Catalysts were prepared from an alkali form of ferrierite, NaK-FER, with a Si/Al = 9 (TOSOH Co., Japan). The monometallic Co-HFER (3 wt. % Co) was obtained by ionexchanging the NH4-FER form with a Co(CH3COO)2 solution. The bimetallic Co/Pd-HFER sample (0.3 wt. % of Pd, 3wt. % Co) was then prepared by ion-exchanging it with a solution of Pd(NH3)4(N03)2. Further details are given elsewhere [10], UV-Vis/RDS spectra were carried out on a Varian Cary 5000 UV-VIS-NIR spectrophotometer. H2-TPR experiments were performed using samples of 130 mg of catalyst, under a mixture of 5% H2/Ar from RT to 1000°C (7.5°C min"1). 

A Varian Cary 100 model UWis spectrophotometer was used for optical absorbance measurements. The absorbance measrrrements were conducted in situ for the study of the kinetics of adsorption process. In all experiments, the size and mass of ACC was kept as constant as possible (about 18.0 0.1 mg). Weighed ACC pieces were pre-wetted by leaving in water for 24 h before use. The idea of using pre-wetted ACC originates from previons findings that pre-wetting enhances the adsorption process [9, 11],... 

UV-Vis absorbance of the solutions was measured on a Varian Cary 50 Spectrophotometer from 400-1000 nm at the initial introduction of solutions and at the end of the experiment. 

Figure 20-2 (cQ Varian Cary 3E Ultraviolet-Visible Spectrophotometer, (b) Optical train. [Courtesy Varian Australia Ply, lid., Victoria, Australia.]... 

Raman spectra were taken on a home built system, composed of Spectra-Physics 2020 series lasers, coupled with a Dilor XY-800 triple spectrometer and a Whight Instruments nitrogen cooled CCD. All samples were measured at room temperature in a backscattering configuration, with 514.53 nm Ar+ laser excitation. The laser power was tuned between 1 mW and 30 mW. UV-VIS diffuse reflectance spectra were taken on a Varian Cary 5 spectrophotometer, equipped with a specially designed Praying Mantis diffuse reflectance attachment of Harrick. 

Experimental. All photodimerizations were carried out in a stainless steel fixed volume cell (1.75 cm ID with a 1.0 cm path length) with sapphire windows under the irradiation of a Hanovia medium pressure mercury lamp filtered through water and Pyrex for a 13.5 hour exposure. The cell and lamp assembly have been described previously (31). For selected runs a custom built 0.9 mL variable-volume pump was connected to the cell and the pressure was varied to determine the exact location of the phase boundary, based on light scattering measured in a Cary 2290 UV-Vis spectrophotometer (Varian Inst.). The spectrophotometer was also used to measure the concentrations of the monomeric cyclohexenone before and after reaction. 

Figure 1 is the ultraviolet spectrum of a 10 mcg/ml solution of vitamin D3 in methanol. The spectrum was obtained using a Cary Model 219 recording spectrophotometer (Varian Instrument Co., Palo Alto, CA). Vitamin D3 and related compounds have a characteristic UV absorption maximum at 265 nm and a minimum at 228 nm. The extinction coefficient at 265 nm is about 17,500 and 15,000 at 254 nm. An index of purity of vitamin D3 is a value of 1.8 for the ratio of the absorbance at 265 to that at 228 nm. The high absorbance at 254 nm enables one to use the most common and sensitive spectrophotometric detector used in high performance liquid chromatography (HPLC) for the analysis of vitamin D3 in multivitamin preparations, fortified milk, other food products, animal feed additives etc. 

The temperature was indicated and controlled to 0.1°C with a platinum resistance probe which extended 1 mm inside the inner surface of the cell. The pressure was adjusted using a 100 cc Ruska syringe pump and was measured to within 0.1% with a 710A Heise digital pressure gauge which is traceable to an NBS standard. The pressure varied less than 0.15 bar (2 psi) during a spectral scan. The wavelength accuracy was 0.2 nm for the Varian (Cary) 2290 spectrophotometer. 

All UV/vis spectra were recorded on a Varian Cary 50 Scan spectrophotometer and a PERKIN ELMER UV/vis Spectrometer Lambda 2 (double beam) in solution. Absorption maxima Xmax are given in nm. 0.4 cm quartz cuvettes were used for all measurements. 

Figure 6 The structure of the potent quasi-irreversihle CYP3A4 inhibitor troleando-mycin (top panel), the metabolic steps required to convert troleandomycin into a nitroso metabolite that coordinates with the ferrous-heme of CYP3A4 (side panel), and the spectral changes associated with MI complex formation (bottom panel). Troleandomycin (50 pM) was incubated at 37°C with a human liver microsomal sample with high CYP3A4/5 activity (1 mg/mL) andNADPH (1 mM) for the times indicated. The reference cuvette contained the same components minus troleandomycin. Scans from 380 to 520 nm were recorded on a Varian Cary 100 BIO IJV/Vis dual beam spectrophotometer. Abbreviation MI, metabolic intermediate.

All reactions were carried out under an argon atmosphere. The solvents used were of commercial p.a. quality. H and 13C NMR data were obtained on a Bruker ARX 400 spectrometer. Phase transitions were studied by differential scanning calorimetry (DSC) with a Bruker Reflex II thermosystem at a scanning rate of 10 °C min-1 for both heating and cooling cycles. UV-Vis and fluorescence spectra were recorded on a Jasco V-550 spectrophotometer and a Varian-Cary Eclipse spectrometer, respectively. Low-resolution mass spectra were obtained on a Varian... 

The UV-vis-NIR spectra of isolated EMF.v were recorded between 300 and 1100 nm in a toluene solution using a Varian Cary 500 Scan UV-Vis-NIR -spectrophotometer. 

In vivo light absorption spectra (400-700 nm) were recorded with a Varian-Cary spectrophotometer equipped with an integrating sphere. Samples were taken to an approximate optical density of 0.1. Data were corrected for scattering by subtracting a baseline value as measured at 725 nm. The spectrally averaged, chlorophyll a specific absorption cross section (aph, m2 mg Chi a-1) was calculated as follows ... 

Many commercial visible-UV spectrophotometers are suitable for this experiment. These instruments range from simple single-beam devices such as the Spectronic model 20 to high-performance double-beam scanning spectrophotometers such as various Varian-Cary models. The components and operational principles of these instruments are... 

Optical diagram of Varian-Cary models 219 and 2000 series double-beam spectrophotometers (available as models 400 and 500 from Varian). 

CW-EPR X-band measurements were performed on a Bruker ESP 300E Spectrometer at a temperature of 120 K. The CuHis complexes are paramagnetic due to the S=l/2 spin of the Cu " ion. Nitrogen physisorption was performed with a Micromeritics ASAP 2400 apparatus. Measurements were done at 77 K. Prior to the measurements the zeolite samples were degassed for 24 hours at 373 K in vacuum. Micropore volumes and pore size distributions were determined with standard BET and BJH theory. Diffuse Reflectance Spectroscopy of the CuHis complex encapsulated zeolite samples were taken on a Varian Cary 5 UV-Vis-NIR spectrophotometer at room temperature. The DRS spectra were recorded against a halon white reflectance standard in... 

The analysis was performed in a Varian Cary 5 spectrophotometer equipped with a Harrick diffuse reflectance chamber. The first spectra were taken after drying at 473K for 1 h, then after a reduction at 773K for 1 h passing a 20% H2/N2 flow at lOK/min. The spectra were taken at room temperature in the range 200-2000 nm, using zeolite HUSY as reference [9]. 

Diffuse reflection spectroscopy (UV-DRS). UV-Vis diffuse reflectance spectra were recorded in a Varian Cary 5 spectrophotometer (Harrick Scientific) with a diffuse reflectance accessory of Praying Mantis geometry. The samples were set in holds with 2 mm thickness. The spectra were recorded in the 200 nm and 800 nm range with a scanning speed of 1800 mm.min at room temperature against supports as reference. 

Rates of hydrolysis were measured on a Varian Cary 219 UV spectrophotometer equipped with a Haake constant temperature bath. A stirred batch tank reactor was placed in a water bath maintained at 26.0 0.1 C and the solution was transported to a micro flow UV cell located in the spectrophotometer via silicone rubber tubing using a precision flow peristaltic pump (MHRE 22) manufactured by the New Brunswick Scientific Company. 

The prepared catalysts were analyzed by X-ray powder diffraction (XRD), Diffuse Reflectance Spectra (DREAS), Inductive coupled plasma-atomic emission spectrometric analysis (ICP-AES) and scanning electron microscopy (SEM). The catalysts were characterized by XRD before and after calcination, using a Philips PW 1877 automated powder diffractometer with CuKa radiation. DREAS measurements were recorded on a Varian Cary-1 spectrophotometer using BaS04 as a reference. ICP-AES measurements were recorded on a Perkin-Elmer Plasma 40 (ICP) or Perkin-Elmer 1100 (AES). SEM measurements were recorded on a Philips XL-20 microscop. 

All new compounds were fully characterized by spectroscopic data. UV-visible absorption spectra were measured with a Varian Cary 50 spectrophotometer. Spectroscopic measurements were done by using spectral grade solvents at 25 °C. Semi-empirical MO calculations were carried out with the AMI-COSMO method in the MOP AC package (MOPAC2000 ver. 1.0, Fujitsu Ltd, Tokyo, Japan, 1999). 

Tenn. Electronic spectroscopy was performed on a Varian Cary 17 recording spectrophotometer and a Nicolet Fourier Transform Infrared instrument was used to record spectra between 4000 and 200 cm l of samples in Csl. Electrical measurements were performed at room temperature on compacted samples by a four probe technique. TGA was performed on a DuPont 990 Analyzer using a DuPont 951 TGA module. TGA for the GC/MS analysis was performed on a DuPont 950 instrument. Volatile products were collected in a Tenax containing tube which was attached to the GC inlet port, GC/MS was performed on a Hewlett Packard 5982A coupled to a 5934A data system. DSC was performed on a DuPont 1090 analyzer using a DuPont 910 DSC module. 

Bioluminescence spectra. BL spectra were recorded using a Cary-Eclipse luminescence spectrophotometer (Varian) from 400-700 nm wavelengths. 

Spectral measurements. Fluorescence (FL) spectra and UV-Visible absorption spectra were measured on a model Hitachi-F4500 spectrofluorometer (Hitachi, Japan) and a model Cary 50 UV-Visible Spectrophotometer (Varian, USA), respectively. 

Apparatus. The excitation and emission spectra were measured on a Varian Cary-Elips fluorescence spectrophotometer. UV absorption measurements were taken in a Hitachi U-3400 UV-VIS spectrophotometer. The H1 NMR spectra were measured on Bruker AV400 NMR spectrophotometer. 

Cu(N03)2 aqueous solution for one day. The samples were filtered, washed and dried imder ambient conditions. We denote the samples obtained at this stage as CuCli. For the reduction of Cu, heating in a dry H2 flow at temperatures from 150 to 450 °C for 4 hrs was carried out. We denote the samples obtained after such a procedure as Cu" Cli. The copper content in the samples was determined by atomic absorption spectrometry. Diffuse reflectance spectra (DRS) were collected on a Varian Cary 300 spectrophotometer. 

The as-synthesized materials, /. e. micelle-bearing systems, were characterised by XRD and UV-Vis spectroscopy. XRD patterns were recorded using an XTert Philips diffractometer, with CuKa radiation. Reflectance UV-visible spectra were recorded using a Varian Cary 5 spectrophotometer. 

Figure 13-22 shows the optical design of the Varian Cary 100, a more sophisticated, double-beam-in-timo recording spectrophotometer. This instrument employs a 30 X. 3.S mm plane grating having 120()lines/mm. Us range is from 190 to 9(X1 nm. Randwidths of 0.2 to... 

The fluorescence lifetime measurements were performed with a streak camera (Agat SF 3M, VNIIOFI, Russia). A Nd YAG laser with excitation wavelengths of 532 and 266 nm (the second and fourth harmonic of fundamental radiation) was used as a light source. The laser radiation parameters of the fluorimeter were as follows (for 532 nm) pulse energy 160 (ij, duration 20 ps (fwhm), beam diameter 5 mm. The error in determining the fluorescence lifetimes in time intervals of several nanoseconds did not exceed 5 %. In addition to the laser equipment, the Cary 100 spectrophotometer (Varian, Inc., USA) and the Cary Eclipse spectrofluorimeter (Varian, Inc., USA slits width was 5 nm) were used for optical density measurements and fluorescence registration, respectively. 

Intrinsic tryptophan, tyrosine, and phenylalanine fluorescence quenching induced by gold NPs was recorded on a Cary Eclipse fluorescence spectrophotometer (Varian). Excitation was performed at 280 nm. Fluorescence emission was measured at 25°C in DPBS (without Ca and Mg " ) containing various concentrations of gold NPs. The protein concentration was fixed at 0.01 mg/mL. Nanoparticle concentration... 

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