Spectra-Physics

A stainless steel column (4.6 mm internal diameter by 250 mm length) packed with 7 micron Zorbax ODS (Dupont) was equilibrated with 82 % Acetonitrile in water at a flow rate of 2.0 ml/min. provided by a Spectra Physics Model 87(X) pump and controller. The effluent was monitored at 230 nm using either a Tracor UV-Visible detector Model 970A or a Jasco Uvidec UV detector Model 1(X)-V. Peaks were recorded and calculated on a SpectraPhysics recording integrator. Model 4200 or Model 4270. Samples of 0.5 mg/ml in toluene were applied to the column automatically with a Micromeritics Autosampler Model 725 equipped with a 10 pi loop. 

GC-TEA Analysis. A Bendix model 2200 GC and Thermo Electron model 502 TEA were used. The GC injector temperature was 210 C. The TEA pyrolysis furnace was operated at 450 C and the cold trap was held at -150 C in isopentane slush. Oxygen flow to the ozonator was 20 cc/min and indicated pressure was 1.5 torr at a helium flow rate of 20 cc/min. TEA output was processed by a digital integrator (Spectra Physics System I). 

Spectra Physics SP8OOO, SP83IQ U.V,, (25A nm) Schoeffel SF770 UV... 

The dissociation laser light between 230 and 305 nm was produced by frequency doubling the output of an optical parametric oscillator (Spectra-Physics MOPO-730) pumped by an injection-seeded Nd YAG... 

The introduction and diversification of genetically encoded fluorescent proteins (FPs) [1] and the expansion of available biological fluorophores have propelled biomedical fluorescent imaging forward into new era of development [2], Particular excitement surrounds the advances in microscopy, for example, inexpensive time-correlated single photon counting (TCSPC) cards for desktop computers that do away with the need for expensive and complex racks of equipment and compact infrared femtosecond pulse length semiconductor lasers, like the Mai Tai, mode locked titanium sapphire laser from Spectra physics, or the similar Chameleon manufactured by Coherent, Inc., that enable multiphoton excitation. 

For EPy-doped PMMA film, a 308 nm excimer laser (Lumonics TE 430T-2, 6ns) was used as as exposure source. We used a tine-correlated single photon counting systen (18) for measuring fluorescence spectra and rise as well as decay curves of a snail ablated area. The excitation was a frequency-doubled laser pulse (295 nm, lOps) generated from a synchronously punped cavity-dumped dye laser (Spectra Physics 375B) and a CW mode-locked YAG laser (Spectra Physics 3000). Decay curves under a fluorescence microscope were measured by the same systen as used before (19). 

Figure 2.12 Tuning ranges for different dye lasers, illustrating the spectral regions covered by these lasers (pulsed condition) (courtesy of Spectra Physics).

Figure 2.23 The spectral region covered by an OPO system, using as a pumping source the radiation at 355 nm from a Nd YAG laser (courtesy of Spectra Physics).

Spectra-Physics, Autolab Div., San Jose, CA Liquid Chromatograph, Model SP8100 XR Technical Bulletin D/S-01, 12/84. 

Raman spectra were recorded with a computer-controlled Spex 1401 double monochromator equipped with a cooled photomultiplier and photon counting electronics. Excitation was provided by Spectra Physics Kr+ and Ar+ cw lasers. 

Fig. 9. Apparatus for sluijy packing of columns. After the properly fittul column tube is attached to the bottom of the reservoir, both are filled up with the slurry ofthe micropartic-ulate stationary phase. Thereafter a displacement liquid is pumped into the reservoir by the constant pressure pump, e.g., Haskel Model DST>I00, which is driven by preuurized air. Upon displacement, the slurry from the reservoir is filtered over the porous etal frit at the bottom of the column tubing which becomes densely packed with the partic s. By intermit-tently operating the liquid shut-off valve between the pump and the reservoirpressure waves can te generated in order to flirther compact the column packing. Reprinted from Bakalyar et at. U05) with permission from Spectra-Physics.

S. Bakalyar, J. Yuen, and D. Henry, Chromatography Technical Bulletin. TB 114>76, Spectra-Physics, Santa Clara, California. 

A standard HPLC pump (Spectra Physics 8700) is used in constant-pressure mode and pulse-free flow rates from 1 to 5 yL/min are obtained without any modifications of the pump. 

Structural characterization of the surface metal oxide species was obtained by laser Raman spectroscopy under ambient and dehydrated conditions. The laser Raman spectroscope consists of a Spectra Physics Ar" " laser producing 1-100 mW of power measured at the sample. The scattered radiation was focused into a Spex Triplemate spectrometer coupled to a Princeton Applied Research DMA III optical multichannel analyzer. About 100-200 mg of... 

The assay was performed with a HPLC-system consisting of a Spectra-Physics (Spectra Physics, San Jose, CA 95134, USA) model SP8700 solvent delivery system used at a flow rate of l.Oml.min", a Kratos (Kratos Analytical Instruments, Ramsey, NJ 07446, USA) model 757 UV-detector, wavelength 260 nm, range 0.005 aufs, rise-time 1 second. Injections of extracts into a Zymark (Zymark Corporation Inc., Hopkinton, MA 01748, USA) Z 310 HPLC-injection station, equipped with an electrically controlled Rheodyne valve and a 20 pi sample loop, were performed by a Zymate II robot system. The Zymark Z 310 Analytical Instrument Interface was used to control the HPLC-injection station. 

Figure 21. A1) True racemic composition for enantiomer separation196 of frons-2,3-dimethyloxirane by complexation gas chromatography on nickel(II) bis[3-heptafluorobutanoyl-(D )-camphorale] at 80CC. Integration with a Spectra-Physics SP4100 instrument (peak areas are equal).

Figure 1. Analytical HPLC of the phagostimulants. Conditions Partisil 10 ODS-2 (4.6 X 250 mm) column mobile phase, 100% HJO to 100% CHtCN, 2 mL/min sample, 10 /iL 10 mg/mL ambient temperature detector, SP8310 (Spectra-Physics), 254 nm, 0.16 AUFS, 10 MVFS.

Analyses performed on a 10 meter SP2100 capillary column, 50 1 split ratio, linear gas velocity 28.5 cm/sec, T j 120 -> 220°C at 5°/min, Perkin Elmer Sigma 3B w/FID interfaced to Spectra Physics SP4000 Data System. 

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