Slide coating

Slide Coating. A slide coatei, iRustiated in Figure 15, can coat an unlimited number of layers simultaneously. Each layer flows out onto the... 

Static sampling systems are defined as those that do not have an active air-moving component, such as the pump, to pull a sample to the collection medium. This type of sampling system has been used for over 100 years. Examples include the lead peroxide candle used to detect the presence of SO2 in the atmosphere and the dust-fall bucket and trays or slides coated with a viscous material used to detect particulate matter. This type of system suffers from inability to quantify the amount of pollutant present over a short period of time, i.e., less than 1 week. The potentially desirable characteristics of a static sampling system have led to further developments in this type of technology to provide quantitative information on pollutant concentrations over a fked period of time. Static sampling systems have been developed for use in the occupational environment and are also used to measure the exposure levels in the general community, e.g., radon gas in residences. 

The DNA microarrays are spotted on glass slides coated with amino-silane (Coming GAPS II). They should be ready for hybridization immediately when the labeled cDNA is ready. Thus, while the dyes are couplingto the cDNA (step 3 in the previous section), it is recommended to start the following process. 

In IMS, supportive materials, whose surfaces are coated with conductive materials, are used in principal. In the simplest way, the tissue slices can be placed on a metal MALDI plate directly.9 In this case, however, the target plate must be cleaned carefully after the measurement is over. Currently, the method commonly used is that samples are prepared on a disposable plastic sheet or a glass slide coated with series of conductive materials. In particular, a plastic sheet (ITO sheet) or glass slide (ITO glass slide available from Bruker Daltonics K.K., Billerica, MA, or Sigma, St. Louis, MO) coated with ITO (indium-tin oxide) is useful because it has superior optical transparency... 

Figure 21.3 Mouse liver sections (upper panel) on glass slides coated with gold (a) and ITO (b), and mass spectra obtained from them (lower panel). Reprinted with permission from Chaurand et al.12...

Arrays have been produced on filter supports, in microtiter plate wells and on glass slides coated and modified with one-, two- or 3-dimensional surface architectures as shown schematically in Figure 813 19. Glass offers a number of practical advantages, such as mechanical stability and low autofluorescence. Due to the non-porous character of glass chips, the volume of the hybridization solution can be kept to a minimum and probe-target interaction is not limited by diffusion into pores. However, three-... 

V. Afanassiev, V. Hanemann, and S. Wolfl, Preparation of DNA and protein micro arrays on glass slides coated with an agarose film. Nucl. Acids Res. 28, E66 (2000). 

Assuming that an efficient D-A type of molecule can be synthesized, it should be possible to deposit these molecules as a monolayer onto a glass slide coated with a metal such as aluminum or a wide bandgap semiconductor such as Sn(>2. With the acceptor end of the molecule near the conductor and with contact to the other side via an electrolyte solution it should be possible to stimulate electron transfer from D to A and then into the conductor, through an external circuit and finally back to D through the electrolyte. This would form the basis of a new type of solar cell in which the layer of D-A molecules would perform the same function as the p-n junction in a silicon solar cell (50). Only the future will tell whether or not this concept will be feasible but if nature can do it, why can t we ... 

Protocol updated from Krolak et al. (49) and Dashek and Mills (50). Controls (a) Germinate pollen without [14C]-pro and process as before, (b) Construct a radioautographic sandwich with collodion lacking pollen, (c) Expose a slide coated with Kodak NTB-2 Nuclear Track Emulsion to light and develop emulsion with D-19. (Sample autoradiographs are presented in Fig. 3.)... 

Align sections on center of slides coated with 3-aminopropytri ethoxy-silane (silane)—see Fig. 1. 

Thickness Measurement. The thickness of poly(I) at different coverages was obtained using a Tencor alpha-step 100 surface profile measuring system. Electrodes used were glass slides coated with Pt by electron beam evaporation. In order to produce a "step" across which the stylus of the surface profiler was drawn, Apiezon N grease was applied to part of the electrode surface and was removed with CH2CI2 after derivatization with poly(I). 

Cut frozen sections with a cryostat and mount them on glass slides coated with freshly prepared 0.02% poly-L-lysine. Free-floating sections can also be used. 

When using poly-L-lysine or Silane-coated slides in enzyme digestion protocols, the method of their preparation determines the success of section adherence. The slides must be clean, and must be heated prior to deparaffinization. Experimentation can determine the slide coating that provides clean backgrounds with the technique of choice. 

The hydrogel coatings achieved the lowest limits of detection (LTD) 1300 to 1600 amoles/spot, but exhibited significant assay variation (22% intra-slide to 37% inter-slide CV). LLD levels of surface-modified polysfyrene slides (Maxisorb black. Nunc) equaled 1500 amole/spof af 15 to 32% CV, while reflective (mirror-like) slides coated with 3-aminopropyltriethoxysi-lane (Amersham) showed the lowest variahon with CVs at 11 to 14%. 

Slides coated with fused zinc stearate were exposed with the cages to furnish records of the fog deposits, which might be correlated with the kills. 

Cut 5-pm sections from a paraffin block, and mount them on slides coated with APTS... 

Slide coating and curtain coating, schematically depicted in Fig. 3.69, are commonly used to apply multi-layered coatings. However, curtain coating has also been widely used to apply single layers of coatings to cardboard sheet. In both methods, the coating fluid is pre-metered. 

The slides coated with the OOTF were attached to a temperature controlled holder and inserted into a UHV chamber pumped to below 10"8 mbar.31,32 Various lasers were used to eject photoelectrons from the conducting substrate. The lasers wavelengths were chosen so that the photon energy will be above the substrate work function, below the... 

FLEX IHC Microscope Slides (Code K8020) or slides coated with other suitable adhesives... 

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