Nitrocellulose coated slides

Background Comparison of Dyes on Nitrocellulose Coated Slides... 

Hitherto, many methods for the preparation of carbohydrate microarrays have been described, such as nitrocellulose coated slides for noncovalent immobilization of microbial polysaccharides [23], and self-assembled monolayers modified by Diels-Alder mediated coupling of cydopentadiene-derivatized oligosaccharides [24], just to name two. Unfortunately, the first method requires large polysaccharides or lipid modified sugars for the noncovalent interaction. The latter method requires the preparation of oligosaccharides bearing the sensitive cyclopentadiene moiety. 

RebloC solution is manufactured in two strengths mild and strong. It is imperative that the mild solution be used with nitrocellulose-coated slides. The Reblot strong solution may cause the nitrocellulose to detach from the glass slide. 

Figure 3 Noncovalent and nonspecific immobilization of underivatized carbohydrates on a nitrocellulose-coated slide.

Membranes cast upon glass slides also fall into the 3D surface category. Most notable are the nitrocellulose-coated FAST slides offered by Schleicher Schuell (S S) BioScience. A cytokine micro-ELISA product xmder the trade name Provision has been introduced in single-slide and 96-well spacing (64 usable wells) formats. It is called FAST Quant (Harvey, 2003). Historically, nitrocellulose membrane has been used for the sequestering of both proteins and nucleic acids. The adaptation by S S of this microporous (0.2-p pores) material cast into a microarray format has been relatively straightforward. 

Fig. 3. Schematic of staining process of SARS-CoV immunochip. (1) Spotting A high-precision robot transfers the samples, SARS-CoV proteins, and glycans of various complexities, from 96-well plate to nitrocellulose-coated glass slides. (2) Staining Before staining, the slides are rinsed with IX phosphate-buffered saline (PBS), and blocked with 1% bovine serum albumin (BSA)-PBS containing 0.05% NaN3 and 0.05% Tween-20. They are subsequently incubated with horse anti-SARS sera. The primary antibodies captured by microarrays are detected using biotinated anti-horse immunoglobulin (Ig)G, and visualized by Cy3-streptavidin.

In one of the first reports of a carbohydrate microarray, under-ivatized polysaccharides and glycoproteins were immobilized directly on nitrocellulose-coated glass slides (10). In a further demonstration of the feasibility of noncovalent immobilization of underivatized glycans, Willats et al. reported microarrays of glycoproteins, proteoglycans, and polysaccharides on oxidized black polystyrene slides (11). Modified neutral and aiuoiuc dex-tran polysaccharides have also been synthesized and printed on semicarbazide-coated glass slides (12). 

To this end, Wang et al. utilized physical absorption for introducing polysaccharides on solid surfaces [72]. By using nitrocellulose-coated glass slides, they demonstrated that even after extensive washing, a dextran preparation of 20 kDa... 

Nitrocellulose-coated glass slides (FAST slides, Whatman Schleicher Schuell Biosciences, Keene, New Hampshire, UK). 

The investigators showed that the nitrocellulose-coated glass chip is a ready-to-use substrate for carbohydrate microarrays by proving the fact that (i) carbohydrate-containing macromolecules of various structural configuration can be immobilized on a nitrocellulose-coated glass slide without chemical conjugation ... 

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