Silanes, amino

The synthetic scheme typically involves chain-extending addition of protected mononucleotides to a nucleoside bound covalentiy at the 3 -hydroxyl to an inert siUca-based soHd support, such as controlled pore glass (Fig. 11). The initial base-protected 5 -O-dimethoxytrityl (DMT) deoxynucleoside is linked to the soHd support via the reaction of a siUca-bound amino-silane and the -nitrophenylester of the 3 -succinylated nucleoside, yielding a 3 -terminal nucleoside attached to the soHd support (1) (Fig. 11). Chain elongation requites the removal of the 5 -DMT protecting group. 

Wise SA, Chesisr SN, Hertz HS, Hilpert LR, and May WE (1977) A chemically bonded amino-silane stationary phase for the high-performance liquid chromatographic separation of polynuclear aromatic compounds. Anal Chem 49 2306-2310. 

The DNA microarrays are spotted on glass slides coated with amino-silane (Coming GAPS II). They should be ready for hybridization immediately when the labeled cDNA is ready. Thus, while the dyes are couplingto the cDNA (step 3 in the previous section), it is recommended to start the following process. 

Reaction of Cl3SiC6H2-2,4,6-Ph3 with ammonia affords the tris(amino)-silane compound (H2N)3SiC6H2-2,4,6-Ph3 in 71% yield [Eq. 9]." The X-ray crystal structure was the first for a species in which three NH2 groups were bound to one silicon atom. There is pyramidal geometry at the nitrogen atoms. The structural characterization of a series of such Si-NH2 compounds led to the conclusion that N — Si p-d 77-overlap was not significant in the Si-N bonding.99... 

These dendrimers were then used to achieve a stable coating of amino-silanized glass surfaces with NH2-modified DNA-probes (see Fig. 10). DNA chips which were prepared according to this procedure exhibited several advantages regarding the signal-to-noise ratio and their reliability over commercial chips prepared by conventional linking. 

Fig. 9 Covalent attachment of thiol-modified DNA oligomers to amino-silane derivatized supports...

When amino silane primed wafers were baked at 400°C followed by applying polyamic acid, silane had lost its effectiveness as an adhesion promoter. Results are shown in Table VII. On the other hand when polyamic acid solution was spun onto a wafer that was first primed with silane then coated with polyamic acid solution and baked at 400°C, excellent adhesion was retained. 

So far there has been little use in the volume thermoplastics such as PVC and polyolefins. The main exception would seem to be the use of amino-silane treated glass in conjunction with maleated polypropylene in polypropylene compounds. 

Multifunctional photoinitiators, (II), were prepared by Sommerlade et al. (2) for radiation-curable dental compositions using UV light. A Norrish Type visible-light-sensitive multifunctional ketopinic amide derivative attached to a modified amino-silanated resin, (III), was prepared by Condon et al. (3) and used as a macroinitiator in dental applications. 

To summarize, the model used in this paper captures many important features of protein structure and dynamics and is indeed seen to reproduce many of the general trends observed in SM-FRET experiments. At the same time, we have also observed several intriguing discrepancies between the model predictions and the experimental results. One possibility is that these discrepancies originate from shortcomings of the model. For example, the SM-FRET measurements reported in Refs. [30, 33] were performed on a coiled-coil that was immobilized on a positively charged amino-silanized glass surface and involved charged dye molecules. This implies that the protein-surface and donor-acceptor interactions may be dominated by electrostatic forces. Our... 

Fig. 8 (a) Nanopatteming by EBL A silcion substrate is functionalized with an amino-silane and coated with BSA. A focused electron beam is employed to write nanopattems into the BSA film. Proteins from solution can selectively adsorb into the nanopattems and guide the formation of cell-substrate contacts, (b) Fibroblast on fibronectin 10 x 10 nanodot matrix created by electron beam lithography. Cells spread, and fluorescent staining of intracellular proteins shows that focal contacts are located on the nanodots actin green), fibronectin red), and vinculin blue). Areas a, b, and c are shown magnified. (Figure in part reproduced with permission from [103])... 

Octadecylsilane or octadecyldiisobutyl(dimethyI-amino)silane surface modified capillary 10 mM Borate with 29% acetonitrile, pH 8.4 Channel depth 5.2 pm length not specified, chip arrangement 130... 

The trilithium derivatives of tris(amino)silanes, synthesized so far, all have a dimeric structure [1-5]. By treating the tris(amino)silane with three equivalents of BuLi in THF, however, we obtained colorless cystals of the monomeric trilithium compound (Fig. 1,1). 

In the reaction of the dilithiated bis(amino)silanes with PF3 or SiF4, the inorganic four-membered ring systems are isolated [11] (Scheme 1). 

Fig. 10. Reaction model of amino silanes with diepoxides...

The adhesion of PI to Cr as measured by peel strength is excellent and does not degrade with temperature cycling, thermal shock, or high humidity adhesion to TiW and Mo is about 1/3 to 1/2 that of Cr and an amino-silane adhesion promoter is required to obtain acceptable adhesion of PI to AI2O0 and to prevent degradation of adhesion after temperature shock and humidity ... 

SYNS SILANE 48-12 TETRAKIS SILANETETRAM-INE, OCTAMETHYL- TETRAKIS(DIMETHYL-AMINO)SILANE... 

Fig. 3.1 (a) Representative AFM image of NS aggregate on amino-silanized glass slides functionalized with TEA. (b) Profile of cross section of the same NS aggregate showing distances between NS estimated by using the highest point of NS curvature as the center of the particle... 

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