SAED

Sulfosuccinimidyl-2-(7-azido-4-methylcoumarin-3-acetamide)ethyl-l,3 -dithiopropionate (SAED) is a photoreactive heterobifunctional crosslinking agent that also contains a fluorescent group (Thermo Fisher). The sulfo-NHS ester end of the reagent reacts with primary amines in proteins and other molecules to form stable amide linkages. The photoreactive end is an AMCA 

Sulfosuccinimidyl-2-(7-azido-4-methyl coumarin-3-acetamide)ethyl-1,3 -dithiopropionate MW 621.6 23.6 A 

Additional applications of SAED include study of the ryanodine receptor (Yano et al., 2005 Mochizuki et al., 2007) and investigating the protein organization of the postsynaptic density (Liu et al., 2006). 

The extinction coefficient at 298 nm for the same concentration of SAED in the identical solvent is 13,625 M icm i. 

SAED contains a sulfo-NHS ester with a negatively charged sulfonate group on its ring. The presence of this negative charge does lend some expected water solubility to 

Since the cross-linker is cleavable, SAED provides a means of fluorescent transfer of the coumarin tag to a second molecule, which interacts with the initially modified protein (Fig. 181). For example, soybean trypsin inhibitor (STI) was labeled with SAED and then allowed to interact with trypsin. After photoreactive cross-linking of the two interacting molecules, the complex was reduced with DTT, breaking the conjugate and transferring the fluorescent tag to trypsin near the STI binding site (Thevenin et al., 1991). 

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Outlet Superheater (SH) header of Unit 3 (600 MW. supercritical multi-fuel l of an ENEL power station it consists of 2 twin and independent bodies (22 m length, 488.5 mjn internal diameter, 76.2 mm thickness material SA 430 TP 321H stairdess steel). This header has suffered from relevant cracking problems in assembly welds after 108.000 hours of service and... 

Figure 5 provides an example of AE monitoring data from 22.01.1997 to 03,03.1997, in terms of time history of the main plant parameters (fig.Sa), as well as of the AE RMS values (fig. 5b). Normally, very little or no events were recorded, with the exception of the above period, in which an AE activity, very much concentrated in time and space, could be observed a sharp step in cumulative AE events takes place in a short, well defined time interval. A smaller sharp step in EA events had been observed a few days earlier, in the same position. 

Sarle, W.S., (1994), Neural Networks and Statistical Models , Proceedings of the Nineteenth Annual SAS Users Group International Conference, April, 1994. 

This criterion resumes all the a priori knowledge that we are able to convey concerning the physical aspect of the flawed region. Unfortunately, neither the weak membrane model (U2 (f)) nor the Beta law Ui (f)) energies are convex functions. Consequently, we need to implement a global optimization technique to reach the solution. Simulated annealing (SA) cannot be used here because it leads to a prohibitive cost for calculations [9]. We have adopted a continuation method like the GNC [2]. 

The effect of a long axial rectangular emerging defect of width e and of depth h is to increase the section Sa between the solenoid and the tube value of AS = eh. The induction in the space between the solenoid and the external surface of the tube and inside of tube remains unchanged. 

The inspection class SA is based on the requirements of real-time serial inspection that are covered by standard minifocus X-ray tubes. 

The properties of the imaging system that are needed for inspection, correspond to SC3 for SA and SC2 for SB. The resulting table 2 specifies the requirements placed on the entire system for the inspection of light alloys. 

For the inspection of other metallic materials, the requirements of inspection classes SA and SB are shown in table 3a and 3b. For weld inspection the inspection classes SA and SB are based on the requirements of EN 462 and EN 1435. 

Table 3a System Performance for metallic materials-inspection class SA...

The work described in this paper was undertaken as part of a project funded by the European Community under the Industrial and Materials technologies Programme (Brite/EuRam III). The contributions of other partners in the project, AIB-Vinfotte asbl, Tecnatom SA and SLV Mifrichen GmbH) is gratefully acknowledged. 

Fig.5 shows the relation of the echo height F/B and the retio of contact surface area Sa /So. The Sa is the contact surface area, and the So is the contact surface area of the V defectless. The F/B decreased with an increase in the Sa /So, as the ultrasonic wave from the incidence S45C side On the other hand, the F/B as the ultrasonic wave from the incidence Ti side has decreased with an increase in the Sa /So... 

Fig.5 Relation of reflective echo height F/B and retio of contact surface area Sa /So...

Fig.7 shows the relation of the F/B, the temperature T and the time t as the retio of contact surface area Sa /So=50%. O mark is the B echo on the bottom of the upper specimen A mark is the F echo on the bonding interface. The B echo has changed in the changing temperature T. Therefore, the really reflective echo height F/B on... 

The Sa is the bonding interface area. And the calculative value is almost corresponding to the experimential value. This calculative value is described in consideration. 

On the other hand, the intial part of the pulse shapes on the Sa /So=20 ... 

P.Mas, P Delmotte, P. Sas, Proc. of IEEE Instrumentation and Measurement Technology Conference Brussels, June 4-6, 1996. 

BE-1639 Prediction of pressure vessel integrity in creep hydrogen service Mr. P. Bslladon Creusot Loire Industrie SA... 

It is helpful to consider qualitatively the numerical magnitude of the surface tensional stabilization of a particle at a liquid-liquid interface. For simplicity, we will assume 6 = 90°, or that 7sa = 7SB- Also, with respect to the interfacial areas, J sA = SB, since the particle will lie so as to be bisected by the plane of the liquid-liquid interface, and. AB = rcr - The free energy to displace the particle from its stable position will then be just trr 7AB- For a particle of l-mm radius, this would amount to about 1 erg, for Tab = 40 ergs/cm. This corresponds roughly to a restoring force of 10 dyn, since this work must be expended in moving the particle out of the interface, and this amounts to a displacement equal to the radius of the particle. 

Converting to current dQldt where dt is an infinitesimal time interval and to resistance SA one can rewrite this equation in the fomi... 

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