Reversed-phase liquid chromatography-mass spectrometry

Burkhard, L.P., Kuehl, D.W., Veith, G.D. (1985) Evaluation of reverse phase liquid chromatography/mass spectrometry for estimation of n-octanol/water partition coefficients for organic chemicals. Chemosphere 14(10), 1551-1560. 

Reversed phase liquid chromatography-mass spectrometry was applied to extracts of Jamaica Bay (New York) water to determine 1-300 xg/l amounts of nonyl phenol ethoxylates and their metabolites [213]. 

Burkhard, L. R, D. W. Kuehl, and G. D. Veith, Evaluation of Reverse Phase Liquid Chromatography / Mass-Spectrometry for Estimation of V-Octanol / Water Partition Coefficients. Chemosphere, 1985 14, 1551-1560. 

Burkhard LP, Kuehl DW. 1986. N-octanol/water partition coefficients by reverse phase liquid chromatography/mass spectrometry for eight tetrachlorinated planar molecules. Chemosphere 15 163-167. 

Lanina, S.A. Toledo, P. Sampels, S. Kamal-Eldin, A. Jastrebova, J.A. 2007. Comparison of reversed-phase liquid chromatography-mass spectrometry with electrospray and atmospheric pressure ehemieal ionization for analysis of dietary tocopherols. J. Chromatogr. A 1157 159-170. 

Karger, B. L., Kirby, D. P., Vouros, P., Foltz, R. L. and Hidy, B. On-line reversed phase liquid chromatography-mass spectrometry. Anal. Chem. 51 2324-2328, 1979. 

Overall, it is well understood that one method cannot cover the whole metabolome, so the current state-of-the-art demonstrates the need for the application of a number of analytical techniques such as reversed-phase liquid chromatography-mass spectrometry ((RP)LC-MS) untargeted as a general profiling tool, another RPLC-MS method to profile the lipids (on the C30 or C18 column), GC-MS to profile the content of volatiles, and one or two LC-MS or CE-MS methods (targeted and untargeted) to profile the polar metabolites (see Section 5.1). 

See also-. Extraction Solvent Extraction Principles. Gas Chromatography Mass Spectrometry High-Temperature Techniques. Liquid Chromatography Normal Phase Reversed Phase Liquid Chromatography-Mass Spectrometry. Nuclear Magnetic Resonance Spectroscopy - Applicable Elements Nitrogen-15. 

Phase Reversed Phase Liquid Chromatography-Mass Spectrometry. Polarography Organic Appiications. Spectrophotometry Pharmaceuticai Appiications. Thin-Layer Chromatography Oven/iew. X-Ray Fluorescence and Emission X-Ray Diffraction - Singie Crystai. 

Figure 5 Reversed phase HPLC (linear gradient of 10-90% isopropanol in acetonitrile at 25°C) of natural (top) and rearranged (bottom) butterfat triacylglycerols as obtained with light-scattering detector. Peak identification by carbon and double bond number. (Reproduced with permission from Marai L, Kuksis A, and Myher JJ (1994) Reversed-phase liquid chromatography-mass spectrometry of the uncommon triacylglycerol structures generated by randomization of butteroil. Journal of Chromatography A 672 87-99.)...

See also Atomic Absorption Spectrometry Principles and Instrumentation. Atomic Emission Spectrometry Inductively Coupled Plasma. Cosmetics and Toiletries. Derivatization of Analytes. Electrophoresis Is-otachophoresls. Environmental Analysis. Enzymes Overview. Extraction Supercritical Fluid Extraction Solid-Phase Extraction Solid-Phase Microextraction. Ion Exchange Ion Chromatography Applications. Liquid Chromatography Reversed Phase Liquid Chromatography-Mass Spectrometry. Nuclear Magnetic Resonance Spectroscopy - Applicable Elements Carbon-13 Phosphorus-31. Perfumes. 

See also Bioassays Overview. Capillary Electrophoresis Overview. Electrophoresis Oven/iew Principles Two-Dimensional Gels. Gas Chromatography Overview Mass Spectrometry. Immunoassays Overview. Immunoassays, Applications Food. Immunoassays, Techniques Radioimmunoassays Enzyme Immunoassays. Liquid Chromatography Reversed Phase Liquid Chromatography-Mass Spectrometry Food Applications. Mass Spectrometry Oven/iew Principles Matrix-Assisted Laser Desorption Ionization Time-of-Flight. Radiochemical Methods Radioreceptor Assays Food and Environmental Applications. Thin-Layer Chromatography Oven/iew. 

Kuksis, A., Marai, L. and Myher, J. J. (1991a) Reversed phase liquid chromatography-mass spectrometry of complex mixtures of natural triacylglycerols with chloride attachment negative chemical ionisation. J. Chromatogr., 588, 73-87. 

Mittermayr, R. Kahnan, A. Trisconi, M.-J. Heudi, O. Determination of vitamin B5 in a range of fortified food products hy reversed-phase liquid chromatography-mass spectrometry with electrospray ionization. J. Chromatogr. A, 2004,1032,1-6. 

Burkhard, L.P and D.W. Kuehl. -OctanolAVater Partition Coefficients by Reverse Phase Liquid Chromatography/Mass Spectrometry for Eight Tetrachlorinated Planar Molecules, Chemosphere, 15(2) 163-167 (1986). 

Deye JR, Shiveley AN, Oehrle SA, Walters KA. Separation of substituted fullerenes using non-aqueous reversed-phase liquid chromatography—mass spectrometry. J Chromatogr A 2008 1181(1-2) 159-61. 

Karger, B.L. Kirby, D.P. Vouros, R Foltz, R.L. Hidy, B. On-Line Reversed Phase Liquid Chromatography-Mass Spectrometry. Anal. Chem. 1979, 51,... 

Yan, B., Vallier-Douglass, J., Brady, L., Steen, S., Han, M. and Pace, D., et al. Analysis of post-translational modifications in recombinant monoclonal antibody IgCl by reversed-phase liquid chromatography/mass spectrometry. /. Chromatogr. 1164 153-161, 2007. 

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