Chromatography reversed phase liquid

In liquid chromatography, reversed-phase materials such as Cig and Cg are the most commonly used sorbents (429, 430, 434, 438, 446, 447, 453, 454). Examples of baseline separations with reversed-phase columns of several groups of anabolics including stilbenes, resorcyclic acid lactones, and other, frequently used anabolics have been reported (463-466). In addition to reversed-phase separations normal-phase separations of anabolics using either Hypersil (467) and Brownlee (456) silica or diol-modified silica have been reported. Although not all analytes were completely separated, the latter column could be efficiently used to differentiate between estrogenic and androgenic compounds within a mixture of 15 anabolics and their metabolites (468). [Pg.1064]

J. Korpi, D. P. Wittmer, B. J. Sandman, and W. C. Haney, Simultaneous analysis of hydrocortisone and hydrocortisone phosphate by high-pressure liquid chromatography Reversed-phase, ion-pair approach, J. Pharm. Sci., 65 1087 (1976). [Pg.431]

S.6.4.2 Reversed-phase ion-pair liquid chromatography. Reversed-phase ion-pair chromatography is an alternative approach for controlling the retention of ionic compounds. This approach is particularly useful for the separation of amines on silica-based columns and it has had a profound effect on the analysis of this class of compounds. In particular, the combination of reversed-phase ion-pair liquid chromatography and electrochemical detection revolutionized the analysis of neurotransmitters in the brain (Tomlinson et al., 1978 and refs, therein). [Pg.62]

High-pressure liquid chromatography, reversed phase (HPLC, RP) ... [Pg.133]

See also Gas Chromatography Overview. Ion Exchange Ion Chromatography Applications. Isotope Ratio Measurements. Liquid Chromatography Reversed Phase. Mass Spectrometry Mass Separation Stabie isotope Ratio. [Pg.2737]

See also Atomic Absorption Spectrometry Principles and Instrumentation. Atomic Emission Spectrometry Inductively Coupled Plasma. Cosmetics and Toiletries. Derivatization of Analytes. Electrophoresis Is-otachophoresls. Environmental Analysis. Enzymes Overview. Extraction Supercritical Fluid Extraction Solid-Phase Extraction Solid-Phase Microextraction. Ion Exchange Ion Chromatography Applications. Liquid Chromatography Reversed Phase Liquid Chromatography-Mass Spectrometry. Nuclear Magnetic Resonance Spectroscopy - Applicable Elements Carbon-13 Phosphorus-31. Perfumes. [Pg.4721]

See also Bioassays Overview. Capillary Electrophoresis Overview. Electrophoresis Oven/iew Principles Two-Dimensional Gels. Gas Chromatography Overview Mass Spectrometry. Immunoassays Overview. Immunoassays, Applications Food. Immunoassays, Techniques Radioimmunoassays Enzyme Immunoassays. Liquid Chromatography Reversed Phase Liquid Chromatography-Mass Spectrometry Food Applications. Mass Spectrometry Oven/iew Principles Matrix-Assisted Laser Desorption Ionization Time-of-Flight. Radiochemical Methods Radioreceptor Assays Food and Environmental Applications. Thin-Layer Chromatography Oven/iew. [Pg.4878]

The mechanism of SPE is directly related to liquid chromatography toxins have to be retained by the adsorbent and not eluted by the water in the sample. Extraction sorbents are therefore similar to those used in liquid chromatography, reversed-phase ( -alkyl silica, apolar copolymers, graphitized carbon) for aqueous samples, or ion exchangers for charged compounds. Extraction parameters can be directly extrapolated from the retention times of the analytes as measured by liquid chromatography with water as the mobile phase. [Pg.5105]

Proteins High Performance Liquid Chromatography Reverse Phase Liquid Chromatography 44 ... [Pg.579]

High Pressure Liquid Chromatography.—Reverse phase h.p.l.c. using octadecyl bonded groups has been developed for the rapid and efficient separation of nucleosides, nucleotides, and protected oligonucleotides on both analytical and preparative scales. ... [Pg.207]

C18 layers have been found to have clo,se correlation with HP liquid chromatography reversed-phase columns (Gonnet and Marichy, 1979), and TLC is used widely to scout appropriate mobile phases for HPLC (see Chapter 6). Very polar substances (basic and acidic pharmaceuticals) can be separated on RP layers by ion pair chromatography. [Pg.40]