Phaseolus lectins

CARVALHO M R, SGARBiERi V 0 (1998) Relative importance of phytohemagglutinin (lectin) and trypsin-chymotrypsin inhibitor on bean (Phaseolus vulgaris L) protein absorption and utilization by the rat. JNutr Sci Vitaminol (Tokyo). 44 685-96. 

SPARVOLIF, GALLO A, MARiNELLi D, SANTUCCi A, BOLLiNi R (1998) Novel lectin-related proteins are major components in lima bean Phaseolus lunatus L.) seeds. Biochim Biophys Acta. 1382 311-23. 

For the modification of the gold surface different chemical and biological substances (dodecanethiol, lectins, dextran sulfate DS, with the mass of 5 kDa and polyelectrolytes) were used. The mercaptane layer was formed in ethanol atlO" M concentration of thiol. Polyaniline hydrochloride (PAH), Aldrich , USA, was used to cover the transducer surface by a water insoluble polymer. Lectins from Phaseolus vulgaris (PLA), Solanum tuberosum (STA), Helix pomatia (HPA) and Tuberosum vulgaris (WGA) were immobilized on the surface pre-treated with dodecanethiol or PAH. These lectins were used for the immobilization of glycoproteins. Blocking of the free binding sites was carried out with 1% bovine serum albumin (BSA). 

Grant, G., Greer, F., Mackenzie, N. H., and Pusztai, A. Nutritional response of mature rats to kidney bean (Phaseolus valgaris) lectins. J. Sci. Food Agr. 36 409—414, 1985. 

In Fig. 4, we show representative results. First, we detected an antibody reactivity specific for the carbohydrate moieties of an abundant human serum glycoprotein asialo-orosomucoid (ASOR) (Fig. 4B). Second, we found that lectin PHA-L (Phaseolus vulgaris L ) is specific for a defined complex carbohy-... 

Gerfen CR, Sawchenko PE (1984) An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals immunohistochemical localization of an axonally transported plant lectin, Phaseolus vulgaris leucoagglutinin (PHA-L). Brain Res 290 219-238. 

Phaseolus coccineus lectin Phaseolus coccineus GalNAc... 

Phaseolus limensis lectin Phaseolus limensis GalNAc... 

Phaseolus lunatus lectins Phaseolus lunatus (lima bean) GalNAc... 

An alternative approach involves insolubilized glycoproteins. Thus, an affinity system for the isolation of the lectin of red kidney-bean (Phaseolus vulgaris)149,154 involved thyroglobulin-Sepharose, and, for... 

Finally, a group of lectins having a complex, sugar specificity that has yet to be established includes agglutinins from the red kidney-bean (Phaseolus vulgaris),218 the spindle tree(Evonymus europaea L.),217 the scarlet runner-bean (Phaseolus coccineus L.),218 Vicia graminea,219,220 and the meadow mushroom (Agaricus bisporus).221... 

A comparative study of the carbohydrate-binding specificity of four 2-acetamido-2-deoxy-D-galactose-binding lectins (from Dolichos biflorus, Glycine max, Helix pomatia, and Phaseolus lunatus) has been conducted by using a series of model macromolecules for direct precipitation and a variety of mono- and oligo-saccharides as hapten inhibitors.592 The data indicated that the combining site of all four lectins... 

VII. Other Lectins 1. Phaseolus vulgaris (Red Kidney-bean)... 

The lectin from Phaseolus vulgaris L. (var. red), isolated by conventional protein-purification procedures, was shown to be localized in the cytoplasm of the cotyledon and embryo of the seed.243e A glycoprotein (6.6% carbohydrate), the lectin had molecular weight 128,000, and contained high proportions of aspartic acid, serine, and tryptophan, but no cysteine.243e... 

Inhibition of Phaseolus vulgaris Lectin-Erythrocyte Agglutination by Simple and Complex Carbohydrates216,732... 

Fig. 15.—Human IgA, Glycopeptide871 Showing the Carbohydrate-binding Loci for Various Lectins, [a. Limulus polyphemus b, Triticum vulgaris c, Ricinus communis d, Sophora japonica e, Abrus precatorius f, Cytisus sessilifolius g, Phaseolus vulgaris-, h, Canavalia ensiformis i, Lens culinaris j, Pisum sativus k, Vicia faba-, 1, Bandeiraea simplicifolia II m, Solanum tuberosum and n, Ulex europeus II.]...

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