Asialo-orosomucoid

FIG. 2 Atomic force microscopy of plasmid DNA at different stages of condensation with polycations. (a) Circular plasmid DNA (b) DNA condensed with poly-L-lysine (PLL, mol. wt. 4 kDa) at a DNA phosphate/lysine ratio of 2 1 (c) DNA condensed with PLL (mol. wt. 10 kDa) at a phos-phate/lysine ratio of 1 2 (d) toroid of DNA condensed with PLL-asialo-orosomucoid conjugate at a phosphate/lysine ratio of 1 6. [(a) and (d) from Ref. 80, copyright 1998 Oxford University Press (b) and (c) reprinted with permission from Ref. 66, copyright 1999 American Chemical Society.]... 

Fig. 3.—Inhibition of Binding of I-Labeled Asialo-orosomucoid ([ I]ASOR) to Rabbit-liver Membranes by Asialo-orosomucoid (ASOR), Orosomucoid (OR), BSA, and the Neoglycoproteins to Which 1-Thioglucopyranosides had been Attached by Amidin-ation (Glc-BSA). [The subscripts refer to the number of moles of attached 1-thiogluco-pyranosides per mol of BSA (taken from Ref. 97).]...

Fig. 4. Glycan arrays are used to characterize the antibody profiles of vaccinated animals (Glycan array I) and to scan for asialo-orosomucoid (ASOR)-specific immunological probes (Glycan array II). Antigen preparations spotted on each glycan array and their array location are summarized in Supplemental Tables SI and S2 of reference (18) (available at the Physiological Genomics Web site).

In Fig. 4, we show representative results. First, we detected an antibody reactivity specific for the carbohydrate moieties of an abundant human serum glycoprotein asialo-orosomucoid (ASOR) (Fig. 4B). Second, we found that lectin PHA-L (Phaseolus vulgaris L ) is specific for a defined complex carbohy-... 

The rapid, carbohydrate dependent clearance of specifically modified serum glycoproteins, observed in mammalian species (1), was not demonstrable in fish. Of the five proteins examined in Fig. 1, no significantly increased rate of disappearance from the circulation could be correlated with the nature of the terminal, non-reducing glycoside moiety. The marginally faster clearance observed in the case of asialo-orosomucoid, although reproducible, could not be ascribed to the participation of a specific hepatic receptor. This conclusion is supported by the data in Table 1 whereby it is evident that there was no selective accumulation of radioactivity in the liver the major portion of counts recoverable 25 minutes after inj ection were located in the kidneys. 

Orosomucoid Bovine Serum Albumin Asialo- orosomucoid Agalacto- orosomucoid Ahexosamino orosomucoid... 

The DNA is rapidly eliminated from the compartment into which it is administered as a result of both degradation through endo- and exonucleases and distribution to other compartments. The DNA is eliminated from the blood compartment by interaction with Kuppfer cells through a specific scavenger/ receptor interaction (173). An intravenously administered asialo-orosomucoid-polylysine-DNA complex is cleared from the blood with an apparent half-life of 2.5 min (174). The DNA taken up by the liver is eliminated with a half life of 1.0 to 1.3 hours, gene transcripts were evident for 1 to 12 hours, and the gene product persisted for 6 to 24 hours. 

Asialo-orosomucoid inhibits the disappearance of human glucocerebrosidase from the rat circulation following intravenous administration. ... 

Clearance of ribonuclease B from rat circulation depends on specific recognition of the a-D-mannosyl residues of the attached oligosaccharide and is inhibited by the presence of yeast mannan. The clearance from the circulation occurs primarily in liver, spleen, and bone marrow non-parenchymal cells. Quantitative aspects of the hepatic clearance mechanism for the elimination of asialo-fetuin and asialo-orosomucoid by the rat have been described. ... 

Asialo-orosomucoid has been used in a comparative study of the binding of... 

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