Carbohydrate-binding specificities

Teneberg, S., Miller-Podraza, H., Lampert, H. C., Evans, D. J., Jr., Evans, D. G., Danielsson, D., and Karlsson, K. A. (1997). Carbohydrate binding specificity of the neutrophil-activating protein of Helicobacter pylori. ]. Biol. Chem. 272,19067-19071. 

K. J. Dean and C. C. Sweeley, Studies on human liver a-galactosidases. III. Partial characterization of carbohydrate-binding specificities,./. Biol. Chem., 254 (1979) 10006-10010. 

Kornfeld, K., Reitman, M.-L. and Kornfeld, R. (1981). The carbohydrate-binding specificity of pea and lentil lectins. Fucose is an important determinant. Journal of Biological Chemistry, 256,6633-6640. 

Fig. 8.—Carbohydrate-binding Specificity of Con A. (The hydroxyl groups of the a-D-mannopyranosyl group that are most critically involved in binding to con A are italicized. Hydrogen and oxygen atoms believed to participate in hydrogen bonding are overscored.)...

In summary, the lentil lectins exhibit a carbohydrate-binding specificity similar to those of the pea lectin and con A primary specificity is towards a-D-mannopyranosyl residues (2), and secondary is to a-D-... 

Many unanswered questions remain with respect to the lentil lectins. These include the chemical relationship between the two lectins, a comparison of their carbohydrate-binding specificity, a study of the binding of furanoid sugars (for example, a- and /3-arabinofuranosides) to the lectins, and a determination of the H or O atoms of each hydroxyl group which may be involved in the binding phenomenon. There is also the question as to the size of the binding site will the lentil-lectin site be more complementary to sequences of a-D-(l— 2)-mannopyranosyl residues than is con A (compare Ref. 204) ... 

A thorough study of pea-lectin, carbohydrate-binding specificity was conducted by Van Wauwe and coworkers211 (see Table V). By hapten-... 

Studies on the carbohydrate-binding specificity of the fava-bean lectin, as determined by hapten inhibition of hemagglutination,140,213,466-469 showed that the lectin is inhibited by Makela s group III sugars (see Table VI). Hemagglutination by the lectin was inhibited by... 

From the interest expressed in the fava-bean lectin, we may expect to receive more-complete information on both its structure and carbohydrate-binding specificity in the near future. 

Carbohydrate-binding Specificity of Bandeiraea simplicifolia 2-Acetamido-2-deoxy-D-glucose-binding Lectin0... 

Phenyl and p-nitrophenyl /3-D-galactopyranoside, as well as lactose, were poorly inhibitory.19,478 The high lectin-reactivity reported for 2 -O-L-fucosyllactose is interesting, but inconsistent with the observed carbohydrate-binding specificity of the lectin (the authors might well have tested the very active trisaccharide just noted).196... 

In summary, both the physical-chemical and carbohydrate binding-specificity studies on this interesting and important lectin are incomplete additional characterization is required before the lectin can be employed as a meaningful, structural probe. 

Carbohydrate-binding Specificity of Wheat-germ Agglutinin... 

Characterization of the carbohydrate-binding specificity of Ulex europeus II is still very rudimentary. Studies by inhibition of hemagglutination have been completed, using only a few of the appropriate sugars necessary to define specificity of the binding. 

The carbohydrate-binding specificity of purified Dolichos biflorus lectin has been studied in detail by Etzler and Kabat.108 In precipitin studies conducted on the Dolichos lectin, 87-99% was precipitated by ovarian cyst A substance, whereas only 60-70% was precipitated by hog A substance the latter result was attributed to the formation of soluble, lectin-oligosaccharide complexes. At substances were more reactive than A2 substances however, no reaction was observed with B or O substances, even following mild hydrolysis with acid or two stepwise, Smith degradations. N-Deacetylation of A substance with... 

Extensive information on the carbohydrate-binding specificity of the Helix pomatia lectin was obtained by examining the extent to which a large number of sugars inhibited the precipitation reaction between agglutinin and human blood-group A substance or Salmonella typhinurium SH 180 lipopolysaccharide83,178,581,582 (see Table XI). 

A comparative study of the carbohydrate-binding specificity of four 2-acetamido-2-deoxy-D-galactose-binding lectins (from Dolichos biflorus, Glycine max, Helix pomatia, and Phaseolus lunatus) has been conducted by using a series of model macromolecules for direct precipitation and a variety of mono- and oligo-saccharides as hapten inhibitors.592 The data indicated that the combining site of all four lectins... 

Limited data are available with respect to the carbohydrate-binding specificity of abrin. Olsnes and coworkers147 demonstrated that the agglutination of erythrocytes by abrin in the presence of anti-abrin antiserum was inhibited best by D-galactose. Lactose, melibiose, and D-fucose (in the order of decreasing potency) gave weaker inhibition, whereas D-glucose, D-mannose, D- and L-arabinose, D-xylose,... 

Perhaps the most interesting difference between the subunits is their carbohydrate-binding specificity.621,828 BS I(A4) agglutinated type A erythrocytes (specific titer, 170) but not type B cells, and precipitated A substance, indicating an affinity for a-D-linked 2-acetamido-2-deoxy-D-galactosyl residues. Cross-reactivity with a-D-galactosyl res-... 

Figs. 14 to 16 present the structures of a series of naturally occurring glycopeptides, and summarize the carbohydrate-binding specificity of a number of lectins in terms of the carbohydrate units with which they interact. 

---