Dolichos biflorus

Plendl J. and Schmahl W. (1988). Dolichos biflorus agglutinin a marker of the developing olfactory system in a NMRI-mouse strain. Anat Embryol 177, 439-444. 

Plant Hemagglutinin) with Blood Group A Specificity from Dolichos biflorus, Biochemistry (1970) 9, 869. 

Cuatrecasas (45). The Dolichos biflorus lectin was a gift from Dr. Marilynn Etzler (46). Ulex europeus and Bandeirea simplicifolia lectins were purified in our laboratory and iodinated with Nal25i (carrier-free) by Sepharose 4B-bound lactoperoxidase (47)and diazotized iodoaniline coupling (48) procedures, respectively. 

The specific activities of I-labeled Dolichos biflorus lectin and cholera toxin were 1.12 x 108 cpm and 3.1 x 10 cpm per mg of protein, respectively. 

Immunochemical Studies on the Combining Sites of Forssman Hapten Reactive Hemagglutinins from Dolichos biflorus, Helix pomatia, and Wistaria floribunda, D. A. Baker, S. Sugii, E. A. Kabat, R. M. Ratcliffe, P. Hermentin, and R. U. Lemieux, Biochemistry, 22 (1983) 2741-2750. 

Circular dichroism (CD) has been utilized to investigate the effect of saccharides on the conformation of lectins in solution. CD has demonstrated saccharide-induced conformational changes in the lectins from Canavalia ensiformis (7), Dolichos biflorus (8), Ricinus communis (9), and Triticum vulgaris (10). The present study uses circular dichroism to assess the secondary structure of SBA and to measure conformational transitions induced by the saccharides which bind to this lectin. These and previous studies will contribute to a clearer understanding of the unique properties of these sugar-binding proteins. 

An alternative purification of Dolichos biflorus lectin was published by Font and coworkers.518 Fractional precipitation with ammonium... 

The carbohydrate-binding specificity of purified Dolichos biflorus lectin has been studied in detail by Etzler and Kabat.108 In precipitin studies conducted on the Dolichos lectin, 87-99% was precipitated by ovarian cyst A substance, whereas only 60-70% was precipitated by hog A substance the latter result was attributed to the formation of soluble, lectin-oligosaccharide complexes. At substances were more reactive than A2 substances however, no reaction was observed with B or O substances, even following mild hydrolysis with acid or two stepwise, Smith degradations. N-Deacetylation of A substance with... 

A comparative study of the carbohydrate-binding specificity of four 2-acetamido-2-deoxy-D-galactose-binding lectins (from Dolichos biflorus, Glycine max, Helix pomatia, and Phaseolus lunatus) has been conducted by using a series of model macromolecules for direct precipitation and a variety of mono- and oligo-saccharides as hapten inhibitors.592 The data indicated that the combining site of all four lectins... 

Kim and coworkers examined glycopeptides from normal, and cancerous, colonic mucosa.869 Pronase digestion of disrupted cell-membranes gave a soluble glycopeptide fraction. The fractions obtained from normal tissues inhibited Dolichos biflorus, but not Ricinus communis, hemagglutination, whereas the reverse was true of fractions obtained from malignant, colonic mucosa. No further analysis was carried out.869... 

Characterization of the protein glycosylation of Dolichos biflorus lectin was reported by Wuhrer et al. [88]. The protein was digested by means of unspecific... 

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