Peritoneal cells

Moos, A., Oughton, J., and Kerkvliet, N.I., The effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on tumor necrosis factor (TNF) production by peritoneal cells, Toxicol. Lett., 90, 145,1997. 

Some workers have reported a failure of NT to elicit significant histamine release (< 5% at 10 /xM NT) from isolated peritoneal mast cells obtained from Wistar strain rats [82]. In these studies, when NT was added to mast cells from the pleural cavity [82] or when the C-terminal octapeptide (NT6 13) or the C-terminal hexapeptide (NT8 13) was added to peritoneal mast cells, a significant (>20% histamine release) secretory response occurred [82]. In our laboratory a significant difference in the responsiveness to NT of peritoneal and pleural mast cells from Sprague-Dawley rats has also been found, pleural cells eliciting a higher percentage of histamine release than peritoneal cells for an equimolar concentration of NT (19.2 2% release for peritoneal mast cells versus 45 + 6% release for pleural mast cells at 10 /xM NT). Moreover, we have also observed anecdotally differences between various populations of mast cells and between various populations of rats in terms of their responsiveness to the same batch of NT. 

Hydrogenated oil, administered to Listeria monocytogenes-infected mice, produced a significant increase in peritoneal cells of coconut oil-fed mice and a reduction of bacterial recovery from the spleen . Oil, administered to mice injected with a nonlethal dose of Escherichia coli at a dose of 20% by weight for 5 weeks, produced a decrease of peak plasma tumor necrosis factor (TNE)-a, interleukin (IL)-l P, and lL-6 concentrations. Peak plasma IL-10 concentrations were higher in the coconut-fed group than in those fed the other diets, coconut oil diminished production of proinflammatory cytokines in vivo . ... 

Isolated cells from infected mice are less proliferative upon in-vitro activation and increase inducible nitric oxide synthase expression in peritoneal cells, yet splenocytes have normal cytokine responses to ConA (Dai and Gottstein, 1999)... 

Blach-Olszewska, Z. and lanusz, M. 1997. Stimulatory effect of ovine colostrinine (a proline-rich polypeptide) on interferons and tumor necrosis factor production by murine resident peritoneal cells. Arch. Immunol. Ther. Exp. 45, 43-47. 

Kubicka-Muranyi M, Goebels R, Goebel C, Uetrecht J, Gleichmann E. T l5nnphocytes ignore procainamide, but respond to its reactive metabolites in peritoneal cells Demonstration by the adoptive transfer popliteal lymph node assay. Toxicol Appl Pharmacol 1993 122 88-94. 

For evalnation of local tolerability, hnman peritoneal cells (35), hnman osteoblasts (36), and hnman as well as animal endothelial cells (37) have been stndied in culture. The type of toxicity and rank efficacy among various compounds were congruent with the results from earlier studies on other cells (4,23). The clinical relevance of these data remains to be established. 

Bovine corneal cup model/leukocyte chemotactic factors Rat peritoneal cells/histamine release Rat peritoneal mast cells/serotonin release Rat vaginal explant/prostaglandin release Bovine eye cup/histamine and leakotriene C4 release Recovery/repair Chorioallantoic membrane Other... 

Although drug action on elicited macrophages may not necessarily reflect the situation in normal animals, the elicitation procedure was necessitated by the extremely low recovery of peritoneal cells in the absence of such treatment this low recovery would have necessitated the use of very large numbers of mice to accomplish these screening studies. 

Fig. 2. Repeated injections of protein A deplete B-la cells in the PeC. Transgenic Xenomouse mice expressing human Igs [17] were injected intraperitoneally with 0 (open bars), 125 (gray bars), 250 (stippled bars) or 500 p,g (black bars) of recombinant protein A. After 24 h, peritoneal cells were washed and then cells were triple stained with fluorescent antibodies to human IgM (G20-127), mouse CD1 lb (Ml/70), and mouse CD5-PE (53-7.3). FACS analysis allowed identification of the B-la (IgM+CDl lb+CD5+) and B-lb cell subsets (IgM+CDllb+CD5—). All monoclonal antibodies and their corresponding isotype controls were purchased from BD PharMingen. p < 0.05. 

Rodgers KE Ellefson DD (1990) Modulation of respiratory burst activity and mitogenic response of human peripheral blood mononuclear cells and murine splenocytes and peritoneal cells by malathion. Appl Toxicol, 14 309-317. 

Phenylethanoid glycosides from Forsythia fruits were assayed for their inhibitory effect on 5-lipoxygenase from rat peritoneal cells [54], Forsythiaside (27), suspensaside (28), acteoside (1) and p-hydroxyacteoside (29) showed a high inhibitory effect with IC50 of 2.50 x 10 M, 7.97 x 10 M, 5..27 x 10 M and 19.3 x 10 M, respectively. 

Suspensa aside Active as inhibitor of 5-HETE and LTB4 in rat peritoneal cells and human peripheral PMN-L ... 

Figure 4. Uptake of Ca by a mixed rat peritoneal cell suspension. The trace is of the change in absorbance of murexide, 80/iM in the extracellular medium, with ImM Ca. A23187 (6iiM) was added at the point indicated with an arrow (76).

Nacife, V. P., Soeiro, M. D. N. C., Araujo-Jorge, T. C., Neto, H. C. C., and Meirelles, M. D. N. L. (2000). Ultrastructural, immunocytochemical and flow cytometry study of mouse peritoneal cells stimulated with carrageenan. Cell Struct. Fund. 25,337-350. 

Figure 5.11 Culture media levels of immunoreactive (i)TxB2 and i6-keto-PGFi (ngml ) 5 h after in vitro incubation of adherent peritoneal cells (I x lO /ml) from tolerant (TOL) and non-tolerant (control) Long-Evans rats with and without S. mteritidis endotoxin (50/xgml ) (LPS). Values are presented as mean + SEM with 5 animals per group. All values are significantly p < O.OI) different from the control values. Reproduced from Wise et al. by permission of Alan R. Liss, Inc.

Table 6 contains the mean value of three separate experiments. A comparison of the intestinal and peritoneal cell counts reveals that the ampicillin treatment reduced the intestinal cell counts approximately a hundred-fold without affecting the Salmonella titer. Cycasin alone (positive control) resulted in the anticipated rise in MF for typhimurium ampicillin alone did not alter the spontaneous MF, and mice treated with both ampicillin and cycasin were approximately the same as the negative control. The reduction of intestinal microorganisms by ampicillin and the resultant inability to detect mutagenic activity after administration of cycasin parallel the finding in carcinogenic studies. In germ-free animals no tumors were found after administration of cycasin (Spatz et aL, 1967). 

Both spontaneous and zymosan (0.5 mg/ml final concentrationj-stimulated luminol-enhanced chemoluminescence of normal and Trypanosoma cruzi-infected mouse peritoneal cells were inhibited by 100 juM azide and by 0.8 fM superoxide dismu-tase, suggesting the involvement of hemoproteins and superoxide anion in the measured responses (Cardoni et al. 1990). 

There are two main advantages to using oxidized regenerated cellulose (ORC) for this application. Firsdy, since ORC is biocompatible, peritoneal cells beneath the adhesion barrier can repair damaged membranous surfaces while being separated from apposing tissues. Secondly, since ORC is bioabsorbable, the material serves its function as an adhesion barrier and is removed from the implantation site within 4 weeks so that no further foreign-body reactions can occur. 

Hamuro J, Rollinghoff M, Wagner H, Seitz M, Grimm W, Gemsa D (1979) Depressed prostaglandin release from peritoneal cells induced by a T cell adjuvant, lentinan. Z Immun-Forsch 155 248-254... 

Phagocytosis The peritoneal cells (10 cells/200 pi) were preincubated in the presence or absence of AGE for 3 h under 5% CO 2 at 37°C, and then the fluorescent-labeled latex beads solution (1 100 dilution, 0.75 pYG, Polyscience, USA) was added [51J. After one hour incubation, the cells were centrifuged, washed with PBS, and fixed in 2.5%> buffered formalin solution. Macrophages that phagocytoised more than 10 latex beads were counted using Improved Neubauer (Erma, Japan), and the phogocytic activity was determined as the number of activated macrophages per 100 cells [52J. 

SECM lithography of PS brushes, (a) Schematic representation of the local oxidative patterning of PS polymer brushes by a SECM tip electrogenerating Ag + ions in 3M aqueous HNOj. (b) Selective sorting of myofibroblastic and macrophage cells of heterogeneous population of peritoneal cell from their selective adhesion, respectively, onto oxidized PS pattern and on pristine PS. (From Ktari, N., et al., Langmuir, 26,17348-17356,2010.)... 

When given i.v. to mice, poly(ICLC) augmented natural killer cell activity in peritoneal cells, lungs and spleen. Figure 5 shows some typical results. There are many biological response modifiers that can augment NK cell activity, after one or two treatments, but then they lose their effectiveness. Mice do not develop this refractoriness after treat-... 

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