Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Paraffin tumoral tissue

Key words Pharmacogenomic, Paraffin tumoral tissue, Diagnostic technique, Personalized therapy... [Pg.45]

Specht K, Richter T, Muller U, et al. Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embeddded tumor tissue. Am. J. Pathol. 2001 158 419-429. [Pg.69]

Hunter SB, Varma V, Shehata B, et al. Apolipoprotein D expression in primary brain tumors analysis by quantitative RT-PCR in formalin-fixed, paraffin-embedded tissue. J. Histochem. Cytochem. 2005 53 963-969. [Pg.70]

In this technique (http //en.wikipedia.org/wiki/Tissue microarray), a hollow needle is used to remove tissue cores of various diameter (from as small as 0.6 4 mm) from regions of interest in paraffin-embedded tissues (clinical biopsies or tumor samples) (Figs. 13.10, 13.11). [Pg.123]

Thompson ER, Herbert SC, Forrest SM et al. Whole genome SNP arrays using DNA derived from formalin-fixed, paraffin-embedded ovarian tumor tissue. Hum Mutat 2005 26 384-389. [Pg.87]

Recently, Caduff et al. (1999) have evaluated abnormalities in p53 and Ki-ra.v in malignant and borderline ovarian tumors of various histological types in paraffin-embedded tissues. The patterns of these genetic alterations in borderline and malignant neoplasms were compared and correlated with cell type and stage. This preliminary molecular analysis suggests that serous borderline tumors have the same molecular features usually associated with malignancy but are unlikely to represent a precursor of invasive serous carcinoma. On the other hand, mucinous borderline tumors may represent a precursor or variant of mucinous carcinoma of the ovary. [Pg.17]

Intermittent microwave heating has been used for enhancing FISH signals in the paraffin-embedded tissue sections of gastrointestinal neoplasia (see page 458) (Kitayama et al., 2000). The processing of these tumors is known to be particularly difficult because of the presence of necrosis and contamination with inflammatory and normal stroma cells and poor attachment of paraffin sections to the slide. [Pg.222]

Gastric tumor tissue is fixed with 4% neutral formaldehyde for 1 day and embedded in paraffin (Kitayama et al., 2000). Paraffin sections (6 xm thick) are deparaffinized with xylene and rehydrated with ethanol. Centromeric a-satellite DNA probes and locus-specific identifier probes (c-myc and p53) are available from Vyis Inc. (Downers Grove, IL). The probes are labeled with orange (Cy 3) or green (PITC) using digoxigenin-ll-dUTP and nick translation. The sections are placed in 0.01 M citrate buffer (pH 6.0) and heated in a microwave oven for 10 min. This is followed by treatment with 0.2% pepsin in 0.01N HC1 for 10 min at 37°C, and then exposure to 0.1% NP40/2 X SSC for 10 min at the same temperature. [Pg.222]

Ki-67 antigen immunohistochemical staining is a simple and reliable procedure for studying tumor proliferative activity in frozen or formalin-fixed, paraffin-embedded tissues, including archival specimens. This antigen can be retrieved on sections of formalin-fixed and paraffin-embedded tissues by autoclave treatment (Fig. 10.1) or microwave heating (Fig. 10.2). Both methods are reliable and are presented later. [Pg.235]

Polymerase chain reaction (PCR), on the other hand, has several advantages it can be used to analyze small numbers of tumor cells, DNA from formalin-fixed, paraffin-embedded tumor tissue can be used, and it can be automated and standardized. Quantitative PCR techniques are currently being assessed for their clinical application to HF.R-2 DNA testing (Vona et al., 1999). However, presently the PCR technology is not optimally suited for routine, clinical application (see next section for details of quantitative analysis of HER-2Ineu expression). [Pg.290]

Breast tumor tissues are fixed with formalin for 24 hr and embedded in paraffin, and sections are deparaffinized according to standard procedures. They are heated in 0.1 mM sodium citrate buffer (pH 6.0) in a water bath for 40 min at 95°C. After cooling for 20 min, the sections are treated with 0.3% hydrogen peroxide containing 15 mM sodium azide for... [Pg.302]

Indeed, recent reports demonstrated the potential use of enzymatic digestion combined with LC-ESI-MS/MS directly on formalin-fixed paraffin-embedded tissues to identify tumor biomarkers (for a review see [68]). The ultimate hope would be to mobilize such powerful MS tools to routine clinical application for in vitro diagnosis assays. [Pg.617]

Qian X, Jin L, Shearer BM, et al. Molecular diagnosis of Ewing s sarcoma/primitive neuroectodermal tumor in formalin-fixed paraffin-embedded tissues by RT-PCR and fluorescence in situ hybridization. Diagn Mol Pathol. 2005 14 23-28. [Pg.57]

CD30, the most consistent marker of H/RSCs, is readily detected in formalin-fixed, paraffin-embedded tissues. " However, tumor cells in some nonlymphoid malignancies, including embryonic carcinoma, melanoma. [Pg.148]

Epstein AL, Marder RJ, Winter JN, et al. Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone htrman B lymphocytes and derived tumors./ Immunol. 1984 133 1028-1036. [Pg.152]

Levi E, Butmarc J, Kourea HP, et al. Detection of interleukin-2 receptors on tumor cells in formalin-fixed, paraffin-embedded tissues. Appl Immunohistochem. 1997 5 234. [Pg.154]

Litzky LA, Brooks JJ. Cytokeratin immunoreactivity in malignant fibrous histiocytoma and spindle cell tumors comparison between frozen and paraffin-embedded tissues. Mod Pathol. 1992 5 30-34. [Pg.247]

Kernek KM, Brunelli M, Ulbright TM, et al. Fluorescence in situ hybridization analysis of chromosome 12p in paraffin-embedded tissue is useful for establishing germ cell origin of metastatic tumors. Mod Pathol. 2004 17 1309-1313. [Pg.255]


See other pages where Paraffin tumoral tissue is mentioned: [Pg.122]    [Pg.275]    [Pg.419]    [Pg.420]    [Pg.92]    [Pg.93]    [Pg.236]    [Pg.321]    [Pg.251]    [Pg.10]    [Pg.227]    [Pg.237]    [Pg.250]    [Pg.291]    [Pg.293]    [Pg.171]    [Pg.147]    [Pg.67]    [Pg.131]    [Pg.243]    [Pg.410]    [Pg.130]    [Pg.138]    [Pg.9]    [Pg.44]    [Pg.134]    [Pg.151]    [Pg.181]    [Pg.220]    [Pg.259]    [Pg.316]   
See also in sourсe #XX -- [ Pg.52 ]




SEARCH



© 2024 chempedia.info