Molecular diagnosis

Over the last decade, new perspectives have been opened up with the use of natural purified and/or recombinant allergens for the molecular diagnosis of allergy which will provide us with precise information for the diagnosis of cUnical reactions experienced by patients and which are particularly applicable to the diagnosis of anaphylaxis. 

Rizzuto R, Pozzan T (2006) Microdomains of intracellular Ca + molecular determinants and functional consequences. Physiol Rev 86 369-408 Rojo EE, Eernandez JA, Maccioni AA, Jimenez JM, Maccioni RB (2008) Neuroinflammation imphcations for the pathogenesis and molecular diagnosis of Alzheimer s disease. Arch Med Res 39 1-16... 

Yates CR, Krynetski EY, Loennechen T et al. Molecular diagnosis of thiopurine... 

Based on the population genotype-phenotype studies performed to date, assays for the molecular diagnosis of TPMT deficiency have focussed on alleles TPMT 2, TPMT 3A and TPMT 3C, as these represent 80-95% of all mutant alleles of this gene in Caucasians [46, 50]. However, the frequency and pattern of mutant alleles of this gene is different among various ethnic populations. For example, Southwest Asians (Indian, Pakistani) have a lower frequency of mutant TPMT alleles and all mutant alleles identified to date are TPMT 3A (Table 24.1) [52]. This is in contrast to Kenyans and Ghanaians where the frequency of mutant alleles is similar to Caucasians, and all mutant alleles are TPMT 3C (Table 24.1) [53, 54]. Among African Americans, TPMT 3C is the most prevalent allele, but TPMT 2... 

Molecular diagnosis of thiopurine-S-methyltransferase deficiency genetic basis for azathioprine and mercaptopurine intolerance. Ann Intern Med 1997 126 608-614. 

Seifert K A and Levesque C A (2004), Phylogeny and molecular diagnosis of mycotoxigenic fungi , Europ. J. Plant Pathol., 110, 449-471. 

Becker KF, Schott C, Hipp S, et al. Quantitative protein analysis from formalin-fixed tissues implications for translational clinical research and nanoscale molecular diagnosis. /. Pathol. 2007 211 370-378. 

Butler, J.E. (2000b) Solid supports in enzyme-linked immunosorbent assay and other solid-phase immunoassays. In Methods in Molecular Medicine Molecular Diagnosis of Infectious Diseases. (J. Decker, and Reischl, eds.), Vol. 94, pp. 333-372. Humana Press, Inc., Totowa, NJ. 

Yates, C.R., Krynetski, E.Y., Loennechen, T., et al. (1997) Molecular diagnosis of thiopurine S-methyltransferase deficiency genetic basis for azathioprine and mercaptopurine intolerance. Ann. Intern. Med. 126, 608-614. 

Herzog, C. E. and Bates, S. E. (1994) Molecular diagnosis of multidrug resistance. Cancer Treat Res. 73,129-147. 

Niessen, L., Schmidt, H., Miihlencoert, E., Farber, P., Karolewiez, A., and Geisen, R. (2005). Advances in the molecular diagnosis of ochratoxin A-producing fungi. Food Addit. Con-tarn. 22, 324-334. 

It is a truism that accurate pipettes, proper air circulation, and pure chemicals and active enzymes assure success in any analytic procedure. Molecular diagnosis is no exception to this. In addition, because molecular diagnosis often requires exponential target amplification before detection of a sequence of interest, sequestration of amplified materials (post-PCR) from incoming patient samples and preparation... 

Improved PCK/NcoI method for the molecular diagnosis of medium chain acyl-CoA dehydrogenase deficiency using dried blood samples two-stage amplification using two different sets of primers improves accuracy and sensitivity. 

Alves S, Praia MJ, Ferreira F et al. Thiopurine methyltransferase pharmacogenetics alternative molecular diagnosis and preliminary data from Northern Portugal. Pharmacogenetics 1999 9 257-261. 

Wang J, Ban MR, Hegele RA (2005) Multiplex ligation-dependent probe amplification of LDLR enhances molecular diagnosis of familial hypercholesterolemia. J Lipid Res 46 366-372... 

Molecular diagnosis nSSU Hymenolepis nana formed cystic mass in AIDS patient Olson et al. (2003)... 

As it has been shown in previous sections, the use of thick- and thin-film electrodes as supports for genosensor devices offers enormous opportunities for their application in molecular diagnosis. The technologies used in the fabrication of both thick- and thin-film electrodes allow the mass production of reproducible, inexpensive and mechanically robust strip solid electrodes. Other important advantages of these electrodes are the possibility of miniaturisation as well as their ease of manipulation in a disposable manner and therefore the use of small volumes. This is an important issue that makes this methodology for detection of DNA more attractive. 

Drobniewski FA, Watterson SA, Wilson SM, Harris GS. A clinical, microbiological and economic analysis of a national service for the rapid molecular diagnosis of tuberculosis and rifampicin resistance in Mycobacterium tuberculosis. J Med Microbiol 2000 49 271-278. 

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