Normal mouse spleen cells

Jaton, J.-C. Vassalli, P. (1980). A major structural difference between membrane-bound and secreted IgM of normal mouse spleen cells is located in the C-terminal region of their heavy chains. FEBS Lett. 116, 277-280. 

Effect of polycation on migration from capillary tubes of LI210 tumor cells and normal mouse spleen cells... 

OsoBA (1969 a, b) used the Marbrook system to culture varying numbers of normal mouse spleen cells (0.25-4 XIO ) mixed with irradiated spleen cells (to maintain a constant cell density) in the presence of SRC and/or ChRC. He observed that a certain fraction of cultures responded while other remained negative. 

Dutton RWR, Mishell RIR (1967) Cell populations and cell proliferation in the in vitro response of normal mouse spleen to heterologous erythrocytes. Analysis by the hot pulse technique. J Exp Med 126 443-454... 

A select group of studies has helped to shed light on the possible physiological mechanisms by which thymic factors control the expression of functional aspects of immunity. Probably the most consistent effect of thymic factors is their ability to induce suppressor T cell activity in various animal models. For example, TF5 has been shown to induce suppressor cell activity in spleen cells of nude mice for both antibody and cytotoxic T cell responses (Marshall et al., 1981 Ahmed et ah, 1978, 1979). Similar effects have been observed with several purified thymic peptides including Ta (Ahmed, 1978, 1979) and thymulin (summarized in Bach, 1983). Other thymic peptides, such as Taj, appear to be more associated with the generation of functional helper T cells. Similar helper inductive effects have also been observed with thymulin in both normal thymocytes and nude mouse spleen cells, which may result from its ability to enhance the production of T cell growth factor (interleukin-2, IL-2) (Palacios et ah, 1982 Palacios 1983). 

Michell, R.I. Dutton, R.W. (1967). Immunization of dissociated mouse spleen cell cultures from normal mice. Journal of Experimental Medicine, 126, 423-442. 

In the hybridoma method (F-ig. 6-14). a mouse or other small animal is sensiti/cd with an antigen. When a high enough titer of antibody against the selected antigen has been attained, the animal is sacrificed and its spleen cells are collected. The spleen cells contain a large number of B lymphocytes, and it is certain that some will he able to produce antigen-specific antibodies. Because the spleen cells are normal B lymph(x ytcs. they have a very short lifetime in cell cultures. Therefore, a method must be u.sed to extend their lifetime. 

Monoclonal Antibodies from Murine Hybridomas. Murine hybridomas are hybrid cells formed by the fusion of a mouse cancer cell with lymphocytes committed to the secretion of antibodies. The lymphocytes are obtained from the spleens of mice immunized with antigens of interest, e.g. extracellular glycoproteins from a fungal plant pathogen, and under normal circumstances would produce antibodies, but would be unable to divide. Fusion with cancer cells yields hybrids that continue to divide, as well as secrete antibodies. Thus, from a population of lymphocytes from the spleen of an immunized mouse it is possible to generate numerous clones of hybridomas and each clone will yield a single antibody, i.e. monoclonal antibodies. 

Some very interesting differences can exist between an enzyme in cancerous tissue and the analogous enzyme in normal tissues. For instance, the specific activity of purine phosphoribosyl transferase in mouse tumour cells (Ehrlich ascites) had between 15 and 60 times the activity of that in liver, brain, spleen, heart, or kidneys of the same animal (Murray, 1966). Again, the adenylate kinase of a rat hepatoma was 22 times more strongly inhibited than the analogous enzyme from healthy rat muscle using an ATP analogue P P -his-[8- (ethylthio) adenosine-5 -]pentaphosphate (Kappler et at., 1982). 

Martin et al. 2001). Mouse vanin-3 expression is widely found in normal mouse tissue such as spleen, peripheral blood leukocyte, liver, kidney, thymus and heart. Mouse vanin-3 expression is induced by oxidative stress, which is involved in antioxidant response elements as well as mouse vanin-1 gene (Berruyer et al. 2004). The cell lysate showed a pantetheine-hydrolysing activity (Martin et al. 2001) as well as mouse vanin-1 when the enzymatic activity was measured using the lysates of the cells transfected with mouse vanin-3 cDNA. On the other hand, VNN3, a putative human gene homologue of mouse Vanin-3, seems to encode an incomplete protein (Nitto et al. 2008). 

Fig. 9 Fluorescence imaging of lymphocytes in mice. Labeled and/or engineered cells were administrated intravenously (n = 3, 100 pL/mouse, lO cells) and whole body was scanned liom the back side by eXplore Optix, GE Healthcare, Bioscience (excitation at 646 nm, anission 663 nm), 1 h, 2 h, 4 h, 6 h, 48 h, and 1 week after injection. Data woe normalized. SP spleen, LN lymph node of epidermal intestinal tract TM DLD-1 human colon carcinoma, (a) Cy5-labeled cells to the nude mice, (b) Cy5-labeled cells to the DLD-1 implanted nude mice at dorsal division, (c) Both Cy5-labeled and Al-glycan-engineered lymphocytes into the tumor model...

Figure 5.9 Cell-transfer experiment transfer of spleen cells from normal High or Low responders into outbred immuno-suppressed recipients. 3 x 10 SE are injected intravenously together with spleen cells. A X rays 950 rad 24 hours before transfer B Cyclophosphamide treatment 6 mg per mouse intraperitoneally 6 hours before transfer.

The column technique has been applied to lymphocytes from non-im-munized mice as well (Wigzell and Makela, 1970). A mixture of normal lymph node, spleen and bone-marrow cells was passed through an OA-coated column. Column passed cells as well as control cells were transferred to lethally irradiated mice (10 cells per mouse) and followed by immunization with OA and BSA. The antibody content in the sera was detected by the Farr assay. There was no response to OA, while the response to BSA was normal. Control cell suspensions which were not subjected to the column passage resulted in a good response to both OA and BSA (Table 5). 

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