Ehrlich’s ascites

Outline of Purification of UMP Synthase from Ehrlich s Ascites Carcinoma... 

Outline of purification scheme for UMP synthase from Ehrlich s ascites tumor cells of mice. 

Furthermore, mouse model against Ehrlich s ascites tumour (EAT) [36]. This study showed that the treatment with ama-titano-cene 48 increases the lifespan of EAT-bearing mice from 25% to around 50% in a dose-dependent manner, and myelopoiesis (the formation of bone marrow or of blood cells derived from bone marrow) was not suppressed. Additionally, these experiments showed that ansa-titanocene 48 restores the natural killer cell function, which is reduced due to a dysfunction in EAT, and stimulates the natural killer cell-mediated cytotoxicity. 

Fig. 11. Changes in the body weight of Ehrlich s ascites tumor-bearing mice after treatment with antitumor drugs...

Table 10. Antitumor activity of 5-FU and vinyl-type polymeric drugs against Ehrlich s ascites tumor bearing mice"...

Duclauxin (9) was identified (14) as one of the agents responsible for the antitumor activity of culture filtrates of P. stipitatum. It was shown that the phenalenone decreased the proliferation of cells of Ehrlich s ascites carcinoma in vitro, and that the compound exerts its cytotoxic effect on cancer cells by inhibiting the synthesis of nucleic acids without affecting protein synthesis (40). 

FuskovA, a., B. Proksa, and J. Fuska In vitro Effect of Duclauxin and Derivatives of Coumarin on Nucleic Acid and Protein Synthesis in Ehrlich s Ascites Carcinoma (EAC). Pharmazie. 32, 291 (1977). 

Okada, Y., and Tadokoro, J. (1962) Analysis of giant polynuclear cell formarion caused by HVJ virus from Ehrlich s ascites tumor cells. Exp. Cell Res. 26, 98-128. 

PRPP synthetase has been purified from red cells and Ehrlich s ascites cells. The enzymes from both sources require inorganic phosphate and magnesium... 

The in vivo distribution of a new, polymer-nitroxide probe, spin-labelled dextran (termed TEMPO-DX) was investigated by Saito et al, who also compared its distribution and pharmacokinetics to that of 3-carbamoyl-proxyl. As expected, it was found that the half-life of the larger molecule was considerably longer than that of 3-carbamoyl-proxyl, and that the TEMPO-DX concentrated in the tumour of a mouse with an Ehrlich s ascites carcinoma. 

Osterreich, S., Schunck, H., Benndorf, R., Bielka, H. (1991). Cisplatin induces the small heat shock protein hsp25 and thermotolerance in Ehrlich ascites tumor cells. Biochem. Biophys. Res. Comm. 180,243-248. 

Agranofl, B. W., Hajra, A. K. The acyl dihydroxyacetone phosphate pathway for glycerolipid biosynthesis in mouse liver and Ehrlich ascites tumor cells. Proc. Natl. Acad. Sci. U.S. 68, 411-415 (1971). 

Antispasmodic activity. Gum extract, administered to isolated guinea pig ileum at a dose of 3 mg/mL, produced a decrease of spontaneous contraction to 54 7% of control. Exposure of precontracted ileum by acetylcholine, histamine, and KCl to Ferula gum extract produced a concentration-dependent relaxation. Preincubation with in-domethacin, propanolol, atropine, and chlorpheniramine before exposure to the gum, did not produce any relaxation " . Antitumor activity. Water extract of the dried oleoresin, administered by gastric intubation to mice at a dose of 50 mg/ani-mal daily for 5 days, was active on CA-Ehrlich ascites, 53% increase in life span Water extract administered intra-peritoneally was inactive on Dalton s lymphoma, 4.8% ILS, and CA-Ehrlich ascites, 5.5%... 

Antitoxic effect. Sesame oil, adiministered to male Wistar rats, ameliorated hepatic and renal damage in a dose-dependent manner and increased survival in lipopolysaccha-ride-treated rats. It decreased lipid peroxide concentration in serum but not in liver and kidney. Serum nitrite production was unaffected by sesame oil ingestion, and the activity of xanthine oxidase was reduced in the lipopolysaccharide-challenged rats k Anti-tumor activity. Water extract of the dried seed, administered intragastrically to mice at a dose of 50 mg/animal daily for 5 days, was active on CA-Ehrlich-ascites, 18% increase in life-span. Intraperitoneal administration was active on Dalton s lyphoma and CA-Ehrlich-ascites, 19 and 39% increase in life-span, respectively ". Seed oil, administered to rats intraperito-neally with 1,2,5,6-dibenzanthracene or re-tene, was active on sarcoma ". 

In die peripheral blood system, crocetin derivatives prevent an elevation in bilirubin levels [3] and also reduce elevated levels of serum cholesterol and triglyceride [4]. Anti-tumor activity of saffron is observed in mice transplanted with several types of tumor cell lines including sarcoma 180, Ehrlich ascites carcinoma, and Dalton s lymphoma ascites... 

Mukherjee, K. Ghosh, S. Ray, M. Ray, S. Purification and characterization of 3-phosphoglycerate kinase from Ehrlich ascites carcinoma cells. Indian J. Biochem. Biophys., 39, 332-341 (2002)... 

Kuznicki J, Filipek A, Hunziker PE, Huber S, Heizmann CW. 1989b. Calcium-binding protein from mouse Ehrlich ascites-tumour cells is homologous to human calcyclin. Biochem J 263(3) 951-956. 

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