Mouse spleen cells

H. Umezawa hoped to obtain immunostimulants from microbes, because, in cancer patients, the immune response is lowered. In 1972, H. Umezawa, T. Takeuchi, and M. Ishizuka (now Vice-Director of the Institute for Chemotherapy, a branch of IMC) found that the administration in mice of a small dose of diketocoriolin B, an oxidation derivative of the antitumor antibiotic coriolin B (1971), increases the number of mouse-spleen cells... 

Astaxanthin has been shown to enhance in vitro antibody production by mouse spleen cells stimulated with sheep red blood cells and in human blood cells in vitro. Furthermore, it has not exhibited any mutagenicity in an in vitro study at doses up to 14.4 mg/day for 2 weeks. 

Oeltmann, T.N., and Forbes, J.T. (1981) Inhibition of mouse spleen cell function by diphtheria toxin fragment A coupled to anti-mouse Thy-1.2 and by ricin A chain coupled to anti-mouse IgM. Arch. Biochem. Biophys. 209, 362. 

Yoshida, T., Shimamura, T., and Shigeta, S., Immunological Effects of Arsenic Compounds on Mouse Spleen Cells In Vitro, Tokai J. Exp. Clin. Med, 11, 353, 1986. 

Kaminski NE, Abood ME, Kessler FK, Martin BR, Schatz AR. (1992). Identification of a functionally relevant cannabinoid receptor on mouse spleen cells that is involved in cannabinoid-mediated immune modulation. Mol Pharmacol. 42(5) 736-42. 

Cells in the appropriate medium for this example, isolated mouse spleen cells washed and resuspended in PBS at approx 1 x 10 cells/mL. 

Bom-van Noorloos A. Schipper C., Steenbergen T., Graaf F and Burger E., (1986). Bacteriodes gingivalis activates mouse spleen cells to produce a factor that stimulates resorptive activity of osteoclasts in vitro. Journal of Periodontal Research. Vol 21, 440-444. 

SIM, Sister chromatid exchange, mouse spleen cells in vitro + NT 10000 Zhang et rz/. (1991)... 

Mouse spleen cells Mouse myeloma cells... 

Fig. 4. A mouse spleen cell and tumor cell fuse to form a hybridoma. As the hybridoma divides, it gives rise to a clone of identical cells, giving the name monoclonal" to the antibodies those cells produce (reprinted with permission from Olsen. 1986. p. 27. Copyright. National Academy Piessl...

Jaton, J.-C. Vassalli, P. (1980). A major structural difference between membrane-bound and secreted IgM of normal mouse spleen cells is located in the C-terminal region of their heavy chains. FEBS Lett. 116, 277-280. 

Fig. 8.26. The use of propidium iodide to exclude dead cells from analysis of a mouse spleen cell population for the expression of the Thy-1 surface antigen. The cells were stained with fluorescein for Thy-1 and then with propidium iodide to mark the dead cells. It can be seen that the dead cells (upper right quadrant) contribute to the fluorescein-positive population. Stained cells courtesy of Maxwell Holscher.

Data on bioactivity on immunocompetent cells provide evidence that D-Ala-deltorphin-I potently (10-9 to 10-11 M) and persistently (up to 4 days) enhances Con A-induced mouse spleen cell proliferation [85]. The peptide increases uptake of thymidine and production of interferon--/ in phytohe-magglutinin-activated human lymphocytes [86] and is 100 times more potent than SNC80 in inhibiting the production of p24 antigen, an index of HIV-1 expression, in Jurkat cells stably transfected with a cDNA encoding for the delta-opiate receptor [87]. 

Otani, H. and Monnai, M. 1995. Induction of an interleukin-1 receptor antagonist-like component produced from mouse spleen cells by bovine kappa-caseinoglycopeptide. Biosci. Biotechnol. Biochem. 59, 1166-1168. 

Monoclonal antibodies generated by fusing mouse spleen cells to myeloma cells can be considered as a pure single reagent that is available in unlimited quantity. Drawbacks are high costs, clonal drift and often low affinity. 

Cells in which chemiluminescence has been reported to originate include phagocytic cells, mouse spleen cells (Peterhans et al., 1980), rat thymocytes (Wrogemann et al., 1978), human NK cells (Roder et al., 1982), erythrocytes (canine) (Peerless and Stiehm, 1986), human epidermal cells (Fischer and Adams, 1985), Lettre ascites tumour cells (Mehta et al., 1985), colonic epithelial cells (Crawen et al., 1986) and Walker carcinosarcoma cells (Leroyer et al., 1987). 

Carbodiimides are also employed to couple ovalbumin to mouse spleen cells/ and meli-bionic acid to serum albumin. Aldehyde dehydrogenase, on treatment with DCC, loses its... 

Morphine given by slow-release pellet has been shown to block the capacity of mouse spleen cells to mount an ex vivo antibody response to the artificial antigen, SRBCs, and a naltrexone releasing pellet blocks the effect of the opioid (Bussiere et al., 1992). Serum antibody responses to... 

A -THC, like morphine, has been shown to suppress in vitro antibody formation by mouse spleen cells (Kaminski et al., 1992 Kaminski et al., 1994). Interestingly, A -THC is reported to increase IgGl responses to Legionella pneumophila in drug-treated animals, presumably by polarizing the immune response towards a Th2 phenotype (see below under T cell responses) (Newton et al., 1994). 

More recently Kajimura et al. reports a defensive effect of AR in young mice (5-12 weeks), including its protective effect against Japanese encephalitis virus infection, the enhancement of antibody production by mouse spleen cells, and a beneficial effect on the function of peritoneal exudate cells [160,161]. In this studies two polysaccharides (F-8 and F-9), isolated from the crude polysaccharides AR fraction, enhance the IgM antibody production in aged mice by oral administration. 

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